| a review of studies on animal reservoirs of the sars coronavirus. | in this review, we summarize the researches on animal reservoirs of the sars coronavirus (sars-cov). masked palm civets were suspected as the origin of the sars outbreak in 2003 and was confirmed as the direct origin of sars cases with mild symptom in 2004. sequence analysis of the sars-cov-like virus in masked palm civets indicated that they were highly homologous to human sars-cov with nt identity over 99.6%, indicating the virus has not been circulating in the population of masked palm civets ... | 2008 | 17451830 |
| synthetic recombinant bat sars-like coronavirus is infectious in cultured cells and in mice. | defining prospective pathways by which zoonoses evolve and emerge as human pathogens is critical for anticipating and controlling both natural and deliberate pandemics. however, predicting tenable pathways of animal-to-human movement has been hindered by challenges in identifying reservoir species, cultivating zoonotic organisms in culture, and isolating full-length genomes for cloning and genetic studies. the ability to design and recover pathogens reconstituted from synthesized cdnas has the p ... | 2008 | 19036930 |
| immunogenicity difference between the sars coronavirus and the bat sars-like coronavirus spike (s) proteins. | sars-like coronavirus (sl-cov) in bats have a similar genomic organization to the human sars-cov. their cognate gene products are highly conserved with the exception of the n-terminal region of the s proteins, which have only 63-64% sequence identity. the n-terminal region of coronavirus s protein is responsible for virus-receptor interaction. in this study, the immunogenicity of the sl-cov s protein (s(sl)) was studied and compared with that of sars-cov (s(sars)). dna immunization in mice with ... | 2009 | 19595990 |
| immunogenicity of the spike glycoprotein of bat sars-like coronavirus. | a group of sars-like coronaviruses (sl-cov) have been identified in horseshoe bats. despite sl-covs and sars-cov share identical genome structure and high-level sequence similarity, sl-cov does not bind to the same cellular receptor as for sars-cov and the n-terminus of the s proteins only share 64% amino acid identity, suggesting there are fundamental differences between these two groups of coronaviruses. to gain insight into the basis of this difference, we established a recombinant adenovirus ... | 2010 | 20960282 |
| a real-time pcr assay for bat sars-like coronavirus detection and its application to italian greater horseshoe bat faecal sample surveys. | bats are source of coronaviruses closely related to the severe acute respiratory syndrome (sars) virus. numerous studies have been carried out to identify new bat viruses related to sars-coronavirus (bat-sars-like covs) using a reverse-transcribed-polymerase chain reaction assay. however, a qualitative pcr could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. in this work an sybr green-real time pcr assay was developed for diagnosing ... | 2011 | 22654650 |
| isolation and characterization of a bat sars-like coronavirus that uses the ace2 receptor. | the 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (sars-cov) was one of the most significant public health events in recent history. an ongoing outbreak of middle east respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that their distribution is wider than previously recognized. although bats have been suggested to be the natural reservoirs of both viruses, attempts to isolate the progenitor virus of sars-cov from bats have bee ... | 2013 | 24172901 |
| identification of immunogenic determinants of the spike protein of sars-like coronavirus. | bat sars-like coronavirus (sl-cov) has a genome organization almost identical to that of sars-cov, but the n-terminus of the spike (s) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against covs, of the two viruses has only 63-64% sequence identity. although there have been reports studying the overall immunogenicity of ssl, knowledge on the precise location of immunodominant determinants for ssl is still lacking. in this study, using a series of tr ... | 2013 | 23575730 |