| rous sarcoma virus-transformed avian cells express four different cell surface antigens that are distinguishable by a cell-mediated cytotoxicity-blocking test. | japanese quails bearing avian sarcoma virus-induced tumors develop immune spleen cells that are cytotoxic in vitro against virally and chemically transformed cells, as well as against embryonic cells. the cell-mediated cytotoxicity can be blocked by soluble antigens extracted from in vitro cultured cells. the existence of partial as well as total blocking effects in tests with extracts from various transformed and untransformed virus-producing cells makes it possible to distinguish up to four di ... | 1978 | 207774 |
| use of dna-dna annealing to detect new virus-specific dna sequences in chicken embryo fibroblasts after infection by avian sarcoma virus. | labeled, virus-specific dna synthesized in vitro by the virion-associated polymerase of avian sarcoma virus (asv) was used to measure virus-specific sequences in cell dna in three ways: (i) by determining the effect of cell dna upon the reassociation rate of double-stranded polymerase products; (ii) by measuring the kinetics of annealing of single-stranded polymerase product (cdna) to cell dna; or (iii) by measuring the amount of cdna which anneals to a large excess of cell dna. with these three ... | 1974 | 4138674 |
| effects of dexamethasone on the morphogenesis of two mutants of rous sarcoma virus. | japanese quail cells transformed by the replication-defective, bryan high-titer strain of rous sarcoma virus, bh rsv(-)q, clone 3, revealed intracytoplasmic a-type particles after dexamethasone treatment. in the absence of dexamethasone, or when superinfected with a helper virus in the presence or absence of dexamethasone, no such particles were observed. chick embryo cells (cec) infected with a temperature-sensitive sarcoma virus mutant, la 334, coordinately defective for transformation and vir ... | 1978 | 204599 |
| avian retrovirus-induced surface antigens and their cross-reactivity with chemically-transformed cells and primary embryonic cells of japanese quails. | by testing spleen cells from avian leukosis (alv) and avian sarcoma virus (asv)-injected japanese quails in a microcytotoxicity assay against various target cells, we have demonstrated the existence of several target antigens. with non-transformed alv-infected japanese quail cells used as target cells, an avian retrovirus subgroup-specific destruction was obtained when spleen cells from animals infected with either avaian sarcoma or leukosis virus of the same subgroup were employed. this reactio ... | 1977 | 72738 |
| molecular cloning and characterization of gag-, pol-, and env-related gene sequences in the ev- chicken. | using less stringent hybridization conditions and cloned viral dna probes representing the avian sarcoma virus gag, pol, env, and long terminal repeat (ltr) gene sequences, we detected related sequences in two avian species purportedly lacking all endogenous avian leukosis viruses, the ev- chicken and the japanese quail. the blot hybridization patterns obtained with the various probes suggest the presence of between 40 and 100 copies of retrovirus-related sequences in the genomes of these two sp ... | 1986 | 3016330 |
| presence of retrovirus reverse transcriptase-related gene sequences in avian cells lacking endogenous avian leukosis viruses. | using a molecularly cloned viral dna probe representing the entire avian sarcoma virus (asv) reverse transcriptase (pol) gene, we have detected related sequences in dna preparations from two avain species, ev- chickens and japanese quail, previously demonstrated to lack all endogenous avain leukosis viruses. nucleotide sequence homology was detected only when hybridization conditions, which allowed the formation of stable duplexes with as much as 30% base mismatch, were used. no sequence homolog ... | 1985 | 2410912 |
| jun inhibits myogenic differentiation. | myoblasts from skeletal muscle of chicken or japanese quail embryos were infected with avian sarcoma virus 17 (asv-17), a retrovirus carrying the jun oncogene. at high multiplicities of infection asv-17-induced morphologic transformation inhibited fusion of myoblasts into myotubes and stimulated extended replication. the expression of the muscle-specific proteins desmin, myosin and creatine phosphokinase was inhibited in asv-17-infected cultures. immunofluorescent staining detected strong expres ... | 1991 | 1656361 |