| complete genome sequence of duck tembusu virus, isolated from muscovy ducks in southern china. | we report here the complete genomic sequence of the duck tembusu virus (dtmuv) wj-1 strain, isolated from muscovy ducks. this is the first complete genome sequence of dtmuv reported in southern china. compared with the other strains (ta, gh-2, yy5, and zj-407) that were previously found in eastern china, wj-1 bears a few differences in the nucleotide and amino acid sequences. we found that there are 47 mutations of amino acids encoded by the whole open reading frame (orf) among these five strain ... | 0 | 23118450 |
| gexp analyzer-based multiplex reverse-transcription pcr assay for the simultaneous detection and differentiation of eleven duck viruses. | duck viral pathogens primarily include the avian influenza virus (aiv) subtypes h5, h7, and h9; duck hepatitis virus (dhv); duck tembusu virus (dtmuv); egg drop syndrome virus (edsv); duck enteritis virus (dev); newcastle disease virus (ndv); duck circovirus (ducv); muscovy duck reovirus (mdrv); and muscovy duck parvovirus (mdpv). these pathogens cause great economic losses to china's duck breeding industry. | 2015 | 26518004 |
| a polymerase chain reaction assay for detection of virulent and attenuated strains of duck plague virus. | sequence analysis of duck plague virus (dpv) revealed that there was a 528bp (b fragment) deletion within the ul2 gene of dpv attenuated vaccine strain in comparison with field virulent strains. the finding of gene deletion provides a potential differentiation test between dpv virulent strain and attenuated strain based on their ul2 gene sizes. thus we developed a polymerase chain reaction (pcr) assay targeting to the dpv ul2 gene for simultaneous detection of dpv virulent strain and attenuated ... | 2017 | 28860100 |
| a multiplex pcr for detection of six viruses in ducks. | in this study, six pairs of specific primers that can amplify dna fragments of different sizes were designed and synthesized according to viral protein gene sequences published in genbank. then, a multiplex pcr method was established for rapid detection of duck hepatitis virus 1, duck plague virus, duck tembusu virus, muscovy duck parvovirus, muscovy duck reovirus, and duck h9n2 avian influenza virus, and achieve simple and rapid detection of viral diseases in ducks. single pcr was used to confi ... | 2017 | 28728835 |
| pathogenicity and genetic characterization of a duck tembusu virus associated with egg-dropping in muscovy ducks. | duck tembusu virus (dtmuv) has spread to the major duck-farming region in china, causing acute egg-production drop in chinese duck population. in this study, we characterized a dtmuv strain (named gd2014) isolated from an egg-production drop duck farm in guangdong province, south china. the virus was pathogenic to muscovy duck embryos and caused severe egg production drop for laying muscovy ducks. the genome sequence of gd2014 shared 97-99% homologies with other waterfowl-origin tembusu viruses, ... | 2016 | 27354303 |
| isolation and characterization of a novel tembusu virus circulating in muscovy ducks in south china. | duck tembusu virus (dtmuv) is an infectious pathogen that can cause epidemics in egg-laying ducks. here, we isolated and characterized a dtmuv, designated gdlh01, thought to be responsible for the noticeable egg drop in muscovy duck flocks in south china since 2011. the genome sequence of gdlh01 shared 97-99% homology with other avian-origin tembusu viruses, and 99.5% homology with the mosquito-borne strain sdms recently reported in china. phylogenetic analysis based on the nucleotide sequence o ... | 2016 | 27291976 |