| differentiation of paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using pcr and restriction fragment analysis of genes encoding 16s rrna. | a rapid procedure for the identification of paenibacillus larvae subsp. larvae, the causal agent of american foulbrood (afb) disease of honeybees (apis mellifera l.), based on pcr and restriction fragment analysis of the 16s rrna genes (rdna) is described. eighty-six bacterial strains belonging to 39 species of the genera paenibacillus, bacillus, brevibacillus, and virgibacillus were characterized. amplified rdna was digested with seven restriction endonucleases. the combined data from restricti ... | 2002 | 12089057 |
| a pcr-based method that permits specific detection of paenibacillus larvae subsp. larvae, the cause of american foulbrood of honey bees, at the subspecies level. | a reliable procedure for the identification of paenibacillus larvae subsp. larvae, the causal agent of american foulbrood disease of honey bees (apis mellifera l.) based on the polymerase chain reaction (pcr) and subspecies - specific primers is described. | 2004 | 15189284 |
| diagnosis of american foulbrood in honey bees: a synthesis and proposed analytical protocols. | worldwide, american foulbrood (afb) is the most devastating bacterial disease of the honey bee (apis mellifera). because the distinction between afb and powdery scale disease is no longer considered valid, the pathogenic agent has recently been reclassified as one species paenibacillus larvae, eliminating the subspecies designations paenibacillus larvae subsp. larvae and paenibacillus larvae subsp. pulvifaciens. the creamy or dark brown, glue-like larval remains of infected larvae continue to pr ... | 2006 | 17083701 |