monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ...19882838016
polypeptide and rna composition of the reticuloendotheliosis viruses.the rna and polypeptide composition of chick syncytial virus (csv) and duck infectious anemia virus (diav) was investigated and compared to that of reticuloendotheliosis virus (rev) strain t, the prototype of the newly recognized rev group of viruses. csv and diav contain genomic rna species which cosediment with those of rev in sucrose gradients. five or six polypeptides, two of which are glycoproteins, were consistently found in csv and diav preparations. the major nonglycosylated polypeptides ...1975172472
group-specific antigen shared by the members of the reticuloendotheliosis virus complex.the polypeptides of reticuloendotheliosis virus (rev) were separated by gel filtration in the presence of guanidine hydrochloride. the eight peaks obtained by gel filtration were then analyzed by polyacrylamide gel electrophoresis and four appeared to contain single polypeptides. the material identified as p29 was used to prepare antiserum. this protein constitutes the major internal non-glycosylated polypeptide in the virion. double immunodiffusion indicated that the antiserum was specific for ...197656462
specific antigenic relationships between the rna-dependent dna polymerases of avian reticuloendotheliosis viruses and mammalian type c retroviruses.immunoglobulin g directed against the dna polymerase of rauscher murine leukemia virus (r-mulv) could bind to 125i-labeled dna polymerase of spleen necrosis virus (snv), a member of the reticuloendotheliosis virus (rev) species. competition radioimmunoassays showed the specificity of this cross-reaction. the antigenic determinants common to snv and r-mulv dna polymerases were shared completely by the dna polymerases of gross mulv, moloney mulv, rd 114 virus, rev-t, and duck infectious anemia vir ...19806154804
comparative ultrastructural study of four reticuloendothelias viruses.the morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain t are sperical with a diameter of approximately 110 nm. they are covered with surface projections about 6 nm long and 10 nm in diameter. the center-to-center distance of surface projections is about 14 nm. the budding virions con ...1975170410
duck infectious anemia virus associated with plasmodium lophurae. 19724622046
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