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sarcocystis speeri n. sp. (protozoa: sarcocystidae) from the opossum (didelphis virginiana).the north american opossum (didelphis virginiana) is host to at least 3 species of sarcocystis: sarcocystisfalcatula, sarcocystis neurona, and a recently recognized sarcocystis sp. a new name, sarcocystis speeri, is proposed for the third unnamed sarcocystis. immunodeficient mice are an experimental intermediate host for s. speeri. sarcocystis speeri sporocysts are 12-15 x 8-10 microm in size, and its schizonts are found in many organs of mice. sarcocysts of s. speeri are found in skeletal muscl ...199910577729
isolation of sarcocystis speeri dubey and lindsay, 1999 parasite from the south american opossum (didelphis albiventris) from argentina.sarcocystis sporocysts from the intestines of 2 opossums (didelphis albiventris) from argentina were fed to gamma-interferon knockout (ko) and nude mice. protozoal schizonts were seen in brain, liver, spleen, and adrenal glands of mice examined 33-64 days after feeding sporocysts. sarcocysts were seen in skeletal muscles of ko mice 34-71 days after feeding sporocysts. schizonts and sarcocysts were structurally similar to sarcocystis speeri dubey and lindsay, 1999 seen in mice fed sporocysts from ...200010701583
experimental transmission of sarcocystis speeri dubey and lindsay, 1999 from the south american opossum (didelphis albiventris) to the north american opossum (didelphis virginiana).sarcocystis speeri dubey and lindsay, 1999 from the south american opossum didelphis albiventris was successfully transmitted to the north american opossum didelphis virginiana. sporocysts from a naturally infected d. albiventris from argentina were fed to 2 gamma-interferon knockout (ko) mice. the mice were killed 64 and 71 days after sporocyst feeding (daf). muscles containing sarcocysts from the ko mouse killed 71 daf were fed to a captive d. virginiana; this opossum shed sporocysts 11 days a ...200010864267
in vitro cultivation of schizonts of sarcocystis speeri dubey and lindsay, 1999.schizonts of sarcocystis speeri dubey and lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and gamma-interferon knockout mice fed sporocysts from opossums, didelphis albiventris. at least 1 asexual cycle was completed in 3 days. in vitro-grown merozoites were structurally and antigenically distinct from those of sarcocystis neurona and sarcocystis falcatula. culture-derived merozoites of s. speeri were not infective ...200010958438
the south american opossum, didelphis marsupialis, from brazil as another definitive host for sarcocystis speeri dubey and lindsay, 1999.the north american opossum, didelphis virginiana, is a definitive host for at least 3 species of sarcocvstis: s. falcatula stiles 1983, s. neurona dubey, davis, speer, bowman, de lahunta, granstrom, topper, hamir, cummings, suter 1991, and s. speeri dubey and lindsay 1999. in order to identify species of sarcocystis in the south american opossum, d. inarsupialis, sarcocystis sporocysts from the intestines of a naturally infected opossum (d. marsupialis) from brazil were fed to 4 gamma-interferon ...200011155929
sporocyst size of isolates of sarcocystis shed by the virginia opossum (didelphis virginiana).the virginia opossum (didelphis virginiana) is a definitive host for multiple sarcocystis species including sarcocystis neurona, one of the causative agents of equine protozoal myeloencephalitis (epm), a severe, neuromuscular disease of horses. size and morphologic characteristics of isolates of sarcocystis shed by the opossum were examined to determine if differences were useful in discriminating between the isolates and/or species. collections of sporocysts from 17 opossums were molecularly ch ...200111223210
molecular characterization and development of sarcocystis speeri sarcocysts in gamma interferon gene knockout mice.the north american opossum (didelphis virginiana) is the definitive host for at least three named species of sarcocystis: sarcocystis falcatula, sarcocystis neurona and sarcocystis speeri. the south american opossums (didelphis albiventris, didelphis marsupialis and didelphis aurita) are definitive hosts for s. falcatula and s. lindsayi. the sporocysts of these sarcocystis species are similar morphologically. they are also not easily distinguished genetically because of the difficulties of dna e ...201526303093
extensively variable surface antigens of sarcocystis spp. infecting brazilian marsupials in the genus didelphis occur in myriad allelic combinations, suggesting sexual recombination has aided their diversification.sarcocystis neurona and sarcocystis falcatula are very similar species of apicomplexan protozoa that use marsupials of the genus didelphis as definitive hosts. these mammals can serve as definitive hosts not only for these two parasites, but for other sarcocystis such as sarcocystis speeri and sarcocystis lindsayi. sarcocystis shed by opossums (with the exception of s. neurona) can cause disease in a great variety of birds, being commonly associated with acute pulmonary sarcocystosis in zoos. s. ...201323433642
prevalence of sarcocystis species sporocysts in wild-caught opossums (didelphis virginiana).sarcocystis sporocysts were found in intestinal scrapings from 24 (54.5%) of 44 opossums (didelphis virginiana). the number of sporocysts varied from a few (< 10,000) to 245 million. sporocysts from 23 of 24 opossums were fed to captive budgerigars (melopsittacus undulatas), and the inocula from 21 opossums were infective, indicating the presence of sarcocystis falcatula. sporocysts from 24 opossums were fed to gamma-interferon-knockout (ko) or nude mice; inocula from 14 opossums were infective ...200010958444
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