| mycobacterium marinum persists in cultured mammalian cells in a temperature-restricted fashion. | we have explored the relatively rapidly growing animal and human pathogen mycobacterium marinum as an experimental model for mycobacterial pathogenesis. m. marinum, which has a lower temperature for optimal growth than does mycobacterium tuberculosis, has a much shorter generation time and can be safely studied in ordinary laboratory facilities and examined in multiple animal infection models. we have established an in vitro assay for its interaction with eukaryotic cells and shown that it persi ... | 1994 | 8039892 |
| mycobacterium marinum causes both long-term subclinical infection and acute disease in the leopard frog (rana pipiens). | mycobacterium marinum grows at an optimal temperature of 33 degrees c, far lower than that for m. tuberculosis. consequently, m. marinum infection of mammals is restricted largely to the cooler surfaces of the body, such as the extremities, but it causes a systemic infection in a large number of poikilothermic animals. here, we describe a laboratory animal model for m. marinum disease in the leopard frog (rana pipiens), a natural host species. m. marinum causes a chronic granulomatous, nonlethal ... | 1997 | 9009340 |
| zebrafish and frog models of mycobacterium marinum infection. | mycobacterium marinum infection of poikilothermic animals, such as fish and frogs, results in chronic granulomatous diseases that bear many similarities to mycobacterioses in mammals, including tuberculosis. this unit describes three animal models of m. marinum infection that can be used to study basic aspects of mycobacterium-host interactions and granuloma development, as well as trafficking of immune cells in host tissues. protocols are included that describe intraperitoneal infection of adul ... | 2006 | 18770575 |
| dynamic nature of host-pathogen interactions in mycobacterium marinum granulomas. | mycobacterium marinum causes long-term subclinical granulomatous infection in immunocompetent leopard frogs (rana pipiens). these granulomas, organized collections of activated macrophages, share many morphological features with persistent human tuberculous infection. we examined organs of frogs with chronic m. marinum infection using transmission electron microscopy in conjunction with immunohistochemistry and acid phosphatase cytochemistry to better define the bacterium-host interplay during p ... | 2001 | 11705964 |