| ranavirus phylogeny and differentiation based on major capsid protein, dna polymerase and neurofilament triplet h1-like protein genes. | in this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of dna polymerase and neurofilament triplet h1-like (nf-h1) protein gene. using these methods, we were able to differentiate the 6 known ranaviruses--bohle iridovirus (biv), european catfish virus (ecv), epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), frog virus 3 (fv3) and singapore grouper iridovirus (sgiv)--with 3 less ch ... | 2009 | 19694168 |
| quantitation of ranaviruses in cell culture and tissue samples. | a quantitative real-time pcr (qpcr) based on a standard curve was developed for detection and quantitation of ranaviruses. the target gene for the qpcr was viral dna polymerase (dnapol). all ten ranavirus isolates studied (epizootic haematopoietic necrosis virus, ehnv; european catfish virus, ecv; european sheatfish virus, esv; frog virus 3, fv3; bohle iridovirus, biv; doctor fish virus, dfv; guppy virus 6, gv6; pike-perch iridovirus, ppiv; rana esculenta virus italy 282/i02, rev282/i02 and shor ... | 2010 | 21087639 |
| susceptibility of pike esox lucius to a panel of ranavirus isolates. | in order to study the pathogenicity of ranaviruses to a wild european freshwater fish species, pike esox lucius fry were challenged with the following ranavirus isolates: epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), european catfish virus (ecv), pike-perch iridovirus (ppiv), new zealand eel virus (nzeelv) and frog virus 3 (fv3). the fry were infected using bath challenge at 12 and 22 degrees c. significant mortalities were observed at 12 degrees c for ehnv, esv ... | 2009 | 19402450 |
| genomic sequencing of ranaviruses isolated from edible frogs (pelophylax esculentus). | ranaviruses were isolated from wild edible frogs (pelophylax esculentus) during epizootics in denmark and italy. phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the andrias davidianus ranavirus (adrv), common midwife toad ranavirus (cmtv), testudo hermanni ranavirus (thrv), and pike-perch iridovirus (ppiv). | 2017 | 28935748 |
| genome sequence of a ranavirus isolated from pike-perch sander lucioperca. | the pike-perch iridovirus (ppiv) was isolated in finland from apparently healthy pike-perch fingerlings during routine disease surveillance. our phylogenomic analysis revealed that ppiv is the first fish member of a clade of ranaviruses previously described from european and chinese amphibians. | 2016 | 27856591 |
| suppression of rnai by dsrna-degrading rnaseiii enzymes of viruses in animals and plants. | certain rna and dna viruses that infect plants, insects, fish or poikilothermic animals encode class 1 rnaseiii endoribonuclease-like proteins. dsrna-specific endoribonuclease activity of the rnaseiii of rock bream iridovirus infecting fish and sweet potato chlorotic stunt crinivirus (spcsv) infecting plants has been shown. suppression of the host antiviral rna interference (rnai) pathway has been documented with the rnaseiii of spcsv and heliothis virescens ascovirus infecting insects. suppress ... | 2015 | 25747942 |
| susceptibility of the european common frog rana temporaria to a panel of ranavirus isolates from fish and amphibian hosts. | ranaviruses are an emerging group of viruses and have been implicated in an increase of epidemics in susceptible species. they have a wide host range, infecting fish, amphibians and reptiles, with some isolates able to infect multiple species from different animal classes. whilst some information exists on the pathogenicity of ranaviruses to novel hosts, there is none on the pathogenicity of fish ranaviruses to amphibians; this information is needed to develop measures to prevent the further spr ... | 2013 | 23574703 |