| mycobacterium abscessus complex identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry. | we determined that the vitek ms plus matrix-assisted laser desorption ionization-time of flight mass spectrometry using research-use-only (ruo) v.4.12 and in vitro-diagnostic (ivd) v.3.0 databases accurately identified 41 mycobacterium abscessus subsp. abscessus and 13 m. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from middlebrook 7h11 and burkholderia cepacia selective agars. peak analysis revealed three peaks potenti ... | 2015 | 25948607 |
| functional characterization of the mycobacterium abscessus genome coupled with condition specific transcriptomics reveals conserved molecular strategies for host adaptation and persistence. | mycobacterium abscessus subsp. abscessus (mab) is a highly drug resistant mycobacterium and the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. mab is also one of the most deadly of the emerging cystic fibrosis (cf) pathogens requiring prolonged treatment with multiple antibiotics. in addition to its "mycobacterial" virulence genes, the genome of mab harbours a large accessory genome, presumably acquired via lateral gene transfer including homologs shared ... | 2016 | 27495169 |
| proposal that the two species mycobacterium massiliense and mycobacterium bolletii be reclassified as mycobacterium abscessus subsp. bolletii comb. nov., designation of mycobacterium abscessus subsp. abscessus subsp. nov., and emendation of mycobacterium abscessus. | the names 'mycobacterium abscessus subsp. abscessus' and 'm. abscessus subsp. massiliense', proposed by leao et al. 2009, cannot be validly published. the purpose of this note is to provide a description in accordance with the rules of the bacteriological code (1990 revision). moreover, the proposal of the name 'm. abscessus subsp. massiliense' is contrary to the rule 38 and the correct name of this taxon, at the rank of subspecies is m. abscessus subsp. bolletii. | 2010 | 21037035 |
| emended description of mycobacterium abscessus, mycobacterium abscessus subsp. abscessus and mycobacteriumabscessus subsp. bolletii and designation of mycobacteriumabscessus subsp. massiliense comb. nov. | the taxonomic position of members of the mycobacterium abscessus complex has been the subject of intensive investigation and, in some aspects confusion, in recent years as a result of varying approaches to genetic data interpretation. currently, the former species mycobacterium massiliense and mycobacterium bolletii are grouped together as mycobacterium abscessus subsp. bolletii. they differ greatly, however, as the former m. bolletii has a functional erm(41) gene that confers inducible resistan ... | 2016 | 27499141 |
| genomic analysis of mycobacterium abscessus strain m139, which has an ambiguous subspecies taxonomic position. | mycobacterium abscessus is a ubiquitous, rapidly growing species of nontuberculous mycobacteria that colonizes organic surfaces and is frequently associated with opportunistic infections in humans. we report here the draft genome sequence of mycobacterium abscessus strain m139, which shows genomic features reported to be characteristic of both mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. massiliense. | 2012 | 23045507 |
| bacteraemia caused by mycobacterium abscessus subsp. abscessus and m. abscessus subsp. bolletii: clinical features and susceptibilities of the isolates. | mycobacterium abscessus complex (m. abscessus subsp. abscessus and m. abscessus subsp. bolletii) is an emerging pathogen causing various human infections. however, few studies have focused on m. abscessus complex bacteraemia with detailed species differentiation. the clinical characteristics of patients with bacteraemia due to m. abscessus complex treated at national taiwan university hospital from 2005-2012 were evaluated. species identification was performed by molecular methods, and minimum i ... | 2014 | 24718088 |
| species identification of mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. bolletii using rpob and hsp65, and susceptibility testing to eight antibiotics. | to separate mycobacterium abscessus subsp. bolletii from mycobacterium abscessus subsp. abscessus using species identification, and to investigate the in vitro activity of amikacin, cefoxitin, imipenem, levofloxacin, moxifloxacin, clarithromycin, azithromycin, and linezolid against mycobacterium abscessus. | 2014 | 24932856 |
| utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics. | the erm(41) gene confers inducible macrolide resistance in mycobacterium abscessus subsp. abscessus, calling into question the usefulness of macrolides for treating m. abscessus subsp. abscessus infections. with an extended incubation (14 days), isolates with mics of ≥8 μg/ml are considered macrolide resistant by current clsi guidelines. our goals were to determine the incidence of macrolide susceptibility in u.s. isolates, the validity of currently accepted mic breakpoints, and the erm(41) sequ ... | 2015 | 25653399 |
| in vitro antimicrobial susceptibility of nontuberculous mycobacteria in iran. | many species of nontuberculous mycobacteria (ntm) have long been identified as important causes of human disease, the incidence of which is rising. several reports have suggested increasing trend of both in vitro and in vivo resistance to available treatment regimes. the aim of this study was to evaluate antibiotic susceptibility of clinically relevant ntm isolates using standard microbroth dilution test. antimicrobial susceptibility testing was performed following national committee for clinica ... | 2016 | 26468990 |
| antibiotic susceptibility of diverse mycobacterium abscessus complex strains in new south wales, australia. | members of the mycobacterium abscessus complex are emerging pathogens of increasing importance, causing both respiratory and soft tissue infections, but precise speciation is problematic. this study was performed to examine the subspecies and antibiotic susceptibility of m. abscessus complex isolates collected during 2013 at the statewide new south wales mycobacterium reference laboratory (nsw mrl), australia. mycobacterium abscessus subsp. abscessus accounted for more than half of all m. absces ... | 2015 | 26517625 |
| inhaled antibiotics for refractory nontuberculous mycobacteria and non-cystic fibrosis bronchiectasis and the significance of mycobacterium abscessus subsp. abscessus isolation during m. avium complex lung disease therapy. | | 2015 | 26131990 |
| source investigation of two outbreaks of skin and soft tissue infection by mycobacterium abscessus subsp. abscessus in venezuela. | outbreaks of soft tissue or skin infection due to non-tuberculous mycobacteria are reported frequently in scientific journals but in general the infection source in these outbreaks remains unknown. in venezuela, in two distinct outbreaks, one after breast augmentation surgery and another after hydrolipoclasy therapy, 16 patients contracted a soft tissue infection due to mycobacterium abscessus subsp. abscessus. searching for the possible environmental infection sources in these outbreaks, initia ... | 2016 | 26441024 |
| [soft-tissue infection with mycobacterium abscessus subsp. abscessus after liposuction and panniculectomy in the caribbean]. | we report the case of a 36-year-old woman who presented with a surgical site infection with atypical mycobacteria several months after liposuction in the caribbean. postoperative wound infections with mycobacterium abscessus present as subcutaneous nodular abscesses with partly putrid secretion. due to the necessity of specific diagnostic measures, diagnosis is often delayed. treatment is difficult because mycobacterium abscessus is resistant to conventional tuberculostatics and many other antib ... | 2015 | 26016828 |
| erratum for brown-elliott et al., utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics. | | 2016 | 27016591 |
| in vitro comparison of ertapenem, meropenem, and imipenem against isolates of rapidly growing mycobacteria and nocardia by use of broth microdilution and etest. | we compared the activities of the carbapenems ertapenem, meropenem, and imipenem against 180 isolates of rapidly growing mycobacteria (rgm) and 170 isolates of nocardia using the clinical and laboratory standards institute (clsi) guidelines. a subset of isolates was tested using the etest. the rate of susceptibility to ertapenem and meropenem was limited and less than that to imipenem for the rgm. analysis of major and minor discrepancies revealed that >90% of the isolates of nocardia had higher ... | 2016 | 27053677 |
| successful antibiotic treatment of pulmonary disease caused by mycobacterium abscessus subsp. abscessus with c-to-t mutation at position 19 in erm(41) gene: case report. | mycobacterium abscessus complex (mabc) is the most drug resistant of the mycobacterial pathogens. m. abscessus subsp. abscessus encodes a functional erythromycin ribosomal methylase gene, erm(41), causing inducible macrolide resistance. however, some clinical isolates of m. abscessus subsp. abscessus harboring nonfunctional erm(41) were susceptible to macrolide, even after extended incubation of 14 days. loss of function of the erm(41) genes was associated with a t-to-c substitution at position ... | 2016 | 27188784 |
| allele-specific duplex polymerase chain reaction to differentiate mycobacterium abscessus subspecies and to detect highly clarithromycin-resistant isolates. | on the basis of the structural differences of erm, we used a duplex polymerase chain reaction (pcr) to differentiate mycobacterium abscessus subsp. abscessus and subsp. massiliense isolates and to detect the point mutations of 23s rrna gene that confer a high level of resistance to clarithromycin. subsp. massiliense strains occupying almost half of the clinical isolates can be simply identified, and their clarithromycin susceptibility can be rapidly determined. | 2017 | 27514964 |
| phylogenomics of brazilian epidemic isolates of mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains. | rapidly growing, non-tuberculous mycobacteria (ntm) in the mycobacterium abscessus (mab) species are emerging pathogens that cause various diseases including skin and respiratory infections. the species has undergone recent taxonomic nomenclature refinement, and is currently recognized as two subspecies, m. abscessus subsp. abscessus (mab-a) and m. abscessus subsp. bolletii (mab-b). the recently reported outbreaks of mab-b in surgical patients in brazil from 2004 to 2009 and in cystic fibrosis p ... | 2013 | 24055961 |
| further isolation of mycobacterium abscessus subsp. abscessus and subsp. bolletii in different regions of japan and susceptibility of these isolates to antimicrobial agents. | the aim of this study was to genetically analyse mycobacterium abscessus subsp. abscessus (hereafter m. abscessus) and m. abscessus subsp. bolletii (hereafter m. bolletii) isolates from six different regions of japan and to determine the antimicrobial susceptibility of these isolates. subspeciation of 143 clinical isolates of m. abscessus group was done by comparative sequence analysis of the rpob and hsp65 genes and the internal transcribed spacer (its) region. genetic analysis led to the ident ... | 2013 | 23850022 |
| in vitro activity of bedaquiline against nontuberculous mycobacteria in china. | the main goal of our study was to evaluate in vitro drug susceptibility of bedaquiline against six prevalent pathogenic nontuberculous mycobacteria (ntm) diseases in china. in addition, we investigated the potential molecular mechanism contributing to the bedaquiline resistance in these different ntm species. for slowly growing mycobacteria (sgm), bedaquiline exhibited the highest activity against mycobacterium avium, the mic50 and mic90 were 0.03 and 16 mg/l, respectively. of rapidly growing my ... | 2017 | 28242674 |
| evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of mycobacterium abscessus subspecies according to whole-genome sequencing. | this study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the vitek ms plus system for identifying mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. a total of 175 clinical m. abscessus strains were identified by whole-genome sequencing analysis: 139 mycobacterium abscessus subsp. abscessus and 36 mycobacterium abscessus subsp. massiliense the research-use-only (ruo) saramis k ... | 2016 | 27682129 |
| a clofazimine-containing regimen for the treatment of mycobacterium abscessus lung disease. | patients with lung disease caused by mycobacterium abscessus subspecies abscessus (hereafter m. abscessus) typically have poor treatment outcomes. although clofazimine (cfz) has been increasingly used in the treatment of m. abscessus lung disease in clinical practice, there are no published data on its effectiveness for this disease. this study sought to evaluate the clinical efficacy of a cfz-containing regimen for the treatment of m. abscessus lung disease. we performed a retrospective review ... | 2017 | 28348153 |
| mycobacterium abscessus subsp. abscessus is capable of degrading pseudomonas aeruginosa quinolone signals. | pseudomonas aeruginosa employs 2-heptyl-3-hydroxy-4(1h)-quinolone (the pseudomonas quinolone signal, pqs) and 2-heptyl-4(1h)-quinolone (hhq) as quorum sensing signal molecules, which contribute to a sophisticated regulatory network controlling the production of virulence factors and antimicrobials. we demonstrate that mycobacterium abscessus(t) and clinical m. abscessus isolates are capable of degrading these alkylquinolone signals. genome sequences of 50 clinical m. abscessus isolates indicated ... | 2017 | 28303132 |
| mixed infection of mycobacterium abscessus subsp. abscessus and mycobacterium tuberculosis in the lung. | a mixed infection of mycobacterium abscessus subsp. abscessus (mab) and mycobacterium tuberculosis (mtb) in the lung is an unusual clinical manifestation and has not yet been reported. a 61-year-old woman had been treated for mab lung disease and concomitant pneumonia, and was diagnosed with pulmonary tuberculosis (ptb). despite both anti-ptb and anti-mab therapy, her entire left lung was destroyed and collapsed. she underwent left pneumonectomy and received medical therapy. we were able to succ ... | 2017 | 28180105 |
| discrimination of mycobacterium abscessus subsp. massiliense from mycobacterium abscessus subsp. abscessus in clinical isolates by multiplex pcr. | the rapidly growing mycobacterium m. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases and of infections following cosmetic surgery and postsurgical procedures. m. abscessus sensu lato can be divided into at least three subspecies: m. abscessus subsp. abscessus, m. abscessus subsp. massiliense, and m. abscessus subsp. bolletii. clinical isolates of rapidly growing mycobacteria were previously identified as m. abscessus by dna-dna hybridization. more than 30% of ... | 2014 | 24197885 |
| [the analysis of species identification and susceptibility testing of mycobacterium abscessus]. | to evaluate the method of differentiating mycobacterium abscessus subsp. abscessus (subsp. m. abscessus) from mycobacterium abscessus subsp. massiliense (subsp. m. massiliense), and to investigate the activity of different antibiotics in vitro. | 2014 | 25262693 |
| mycobacterium abscessus subsp abscessus lung disease: 'trouble ahead, trouble behind…'. | mycobacterium abscessus subsp abscessus is the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria (ntm) and is also the most feared due to its well-deserved reputation for being refractory to antibiotic therapy. m. abscessus subsp abscessus has multiple innate antibiotic resistance mechanisms, but the most important one described so far is an inducible erythromycin methylase (erm) gene. m. abscessus subsp abscessus isolates may appear macrolide susceptible on ... | 2014 | 25580261 |
| clinical characteristics and treatment outcomes of patients with acquired macrolide-resistant mycobacterium abscessus lung disease. | macrolide antibiotics are mainstays in the treatment of lung disease due to the mycobacterium abscessus complex. although previous studies have reported development of acquired macrolide resistance in this species, limited data are available on the outcomes of lung disease due to macrolide-resistant m. abscessus subspecies abscessus (hereafter m. abscessus). this study evaluated the clinical features, treatment outcomes, and molecular characteristics of macrolide-resistant isolates of m. abscess ... | 2017 | 28739795 |
| association between sequevar and antibiotic treatment outcome in patients with mycobacterium abscessus complex infections in japan. | macrolide susceptibility differs between subspecies in the mycobacterium abscessus complex, likely due to differences in erm(41) sequevars. patients with m. abscessus complex infection generally show poor clinical outcomes in response to antibiotic treatment. here, the association between genotype and treatment outcome was investigated. | 2018 | 29227218 |