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short- and long-term consequences of developmental saline stress: impacts on anuran respiration and behaviour.secondary salinization has been identified as a major stressor to amphibians. exposure to elevated salinity necessitates physiological adjustments and biochemical changes that may be energetically demanding. as such, exposure to non-lethal levels of salinity during development could potentially alter anuran metabolic rates and individual performance in both pre- and post-metamorphic life stages. we investigated the effects of non-lethal levels of salinity on metamorphic traits (time to reach met ...201626998337
strict consistency between genetic and topographic landscapes of the brown tree frog (buergeria robusta) in taiwan.taiwan presents an excellent opportunity to build a phylogeographic paradigm for fine-scaled differentiation occurring within short distances on an single island. due to the limitation of habitat availability on the island, demographic histories of species in taiwan were strongly influenced by glacial-interglacial cycles. nevertheless, there are relatively few studies demonstrating such phylogeographic patterns for islands, especially in subtropical asia. in this study, we aim to construct the g ...201222019937
temperature-dependent acute toxicity of methomyl pesticide on larvae of 3 asian amphibian species.relative to other animal taxa, ecotoxicological studies on amphibians are scarce, even though amphibians are experiencing global declines and pollution has been identified as an important threat. agricultural lands provide important habitats for many amphibians, but often these lands are contaminated with pesticides. the authors determined the acute toxicity, in terms of 96-h median lethal concentrations, of the carbamate pesticide methomyl on larvae of 3 asian amphibian species, the asian commo ...201525959379
the hormonal regulation of color changes in the sexually dichromatic frog buergeria robusta.during the breeding season, dynamic changes in body coloration are regularly observed in the male brown tree frog buergeria robusta. this study investigated the hypothesis that this sexual dichromatism in male b. robusta is mediated through hormonal regulation. frogs were exogenously injected with testosterone (t) or estradiol (e2). this manipulation revealed that the body coloration (hue, brightness, and saturation) of the male frog increased significantly (i.e., the brilliant yellow color deve ...201424769704
anesthesia of tadpoles of the southern brown tree frog (litoria ewingii) with isoeugenol (aqui-s).because anesthesia of amphibian larvae is currently problematic, the aim of this study was to test if a fish anesthetic containing approximately 500 g/l isoeugenol (aqui-s) could anesthetize tadpoles. three concentrations of aqui-s were tested (of commercial solution 10, 20, and 50 microl/l) with 10 tadpoles of the southern brown tree frog (litoria ewingii) per concentration. tadpoles were anesthetized for 15 min in water at 18-20 degrees c. anesthetic criteria included four time points and two ...201425314815
skin ice nucleators and glycerol in the freezing-tolerant frog litoria ewingii.the brown tree frog (litoria ewingii) is the only known southern hemisphere vertebrate that can survive full-body freezing. freezing challenges living organisms in many ways, with ice formation in the body producing a suite of physical and metabolic stresses which can damage cells and tissues. the present study looked at two mechanisms that address some of these stresses: cryoprotectants and ice nucleating agents (inas). skin secretions from l. ewingii were sampled along with microhabitat substr ...201121373937
cold tolerance and overwintering of an introduced new zealand frog, the brown tree frog (litoria ewingii).the overwintering strategy of litoria ewingii in otago, new zealand, was studied under laboratory and field conditions. microhabitat temperature measurements showed that the frogs were often exposed to subzero temperatures. in the laboratory, litoria ewingii tolerated freezing for up to 6 hrs at -1 degrees c, and after the completion of the freezing event (about 1 hr) at -2 degrees c. frogs frozen with insulation survived freezing for 12 hrs at -1 degrees c. frogs supercooled to -1.2 +/- 0.1 deg ...200818075704
functional analysis for gut microbes of the brown tree frog (polypedates megacephalus) in artificial hibernation.annual hibernation is an adaptation that helps many animals conserve energy during food shortage in winter. this natural cycle is also accompanied by a remodeling of the intestinal immune system, which is an aspect of host biology that is both influenced by, and can itself influence, the microbiota. in amphibians, the bacteria in the intestinal tract show a drop in bacterial counts. the proportion of pathogenic bacteria is greater in hibernating frogs than that found in nonhibernating frogs. thi ...201628155661
native amphibian larvae exhibit higher upper thermal limits but lower performance than their introduced predator gambusia affinis.information on the thermal limits and physiology of ectothermic amphibians is crucial to our understanding of their ecology in the natural environment, particularly with predicted global changes in climate. we documented the thermal limits of larvae of three amphibian species native to hong kong, and their introduced, invasive predator, the mosquitofish (gambusia affinis). we then used larvae of the brown tree frog polypedates megacephalus as a model amphibian to further investigate growth, oxyg ...201930975413
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