| real time polymerase chain reaction to diagnose anaplasma marginale in cattle and deer (ozotoceros bezoarticus leucogaster) of the brazilian pantanal. | epizootiological study of anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. to achieve diagnosis of this rickettsia in cattle and campeiro deer of brazilian pantanal, a comparison was made between a real time polymerase chain reaction (rt-pcr) with intercalating sybr green fluorochrome and primers based on msp5 gene of a. marginale; a conventional pcr (c-pcr); and parasitological examination using ... | 2010 | 20943025 |