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detection and molecular characterization of ascarid nematode infection (toxascaris leonina and toxocara cati) in captive asiatic lions (panthera leo persica).the objective of this study was to investigate the ascarid infection in asiatic lions using scat samples, based on microscopic analysis, pcr amplification of the its-2 region of ribosomal dna and sequence analysis of the amplicons. microscopic analysis indicated the presence of eggs of toxascaris leonina in eleven of the sixteen scat samples analysed and in one of these eleven scats eggs of toxocara cati were also detected. in five of the scats eggs were not detectable. the presence of t. leonin ...201222807016
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