| spatio-temporal distribution of tsetse and other biting flies in the mouhoun river basin, burkina faso. | in the mouhoun river basin, burkina faso, the main vectors of african animal trypanosomoses are glossina palpalis gambiensis vanderplank and glossina tachinoides westwood (diptera: glossinidae), both of which are riverine tsetse species. the aim of our study was to understand the impact of landscape anthropogenic changes on the seasonal dynamics of vectors and associated trypanosomosis risk. three sites were selected on the basis of the level of disturbance of tsetse habitats and predominant tse ... | 2010 | 21198714 |
| identification of midgut trypanolysin and trypanoagglutinin in glossina palpalis sspp. (diptera: glossinidae). | a midgut trypanolysin and an agglutinin from glossina palpalis subspecies were isolated and partially characterized using anion-exchange chromatography and polyacrylamide gel electrophoresis. fplc fractions of midgut extracts of glossina palpalis palpalis caused agglutination and lysis of two trypanosome species (trypanosoma congolense and trypanosoma brucei brucei), although glossina palpalis gambiensis caused only agglutination. the trypanolysin and agglutinin were active only in the posterior ... | 1990 | 2092294 |
| does isometamidium chloride treatment protect tsetse flies from trypanosome infections during sit campaigns? | african animal trypanosomosis is a major pathological constraint to cattle breeding across 10 million km2 of sub-saharan west african countries infested by tsetse flies, their cyclic vectors. the release of sterile males (sterile insect technique [sit]) is a potentially important control technique aimed at eliminating the vectors. prior to release, tsetse are generally treated with isometamidium chloride, a trypanocide, to prevent them from transmitting parasites. the present study investigated ... | 2008 | 18498613 |
| sodalis glossinidius (enterobacteriaceae) and vectorial competence of glossina palpalis gambiensis and glossina morsitans morsitans for trypanosoma congolense savannah type. | sodalis glossinidius is an endosymbiont of glossina palpalis gambiensis and glossina morsitans morsitans, the vectors of trypanosoma congolense. the presence of the symbiont was investigated by pcr in trypanosoma congolense savannah type-infected and noninfected midguts of both fly species, and into the probosces of flies displaying either mature or immature infection, to investigate possible correlation with the vectorial competence of tsetse flies. sodalis glossinidius was detected in all midg ... | 2005 | 15942697 |
| inhibition of the dna amplification of trypanosomes present in tsetse flies midguts: implications for the identification of trypanosome species in wild tsetse flies. | the present study was carried out in order to investigate if there was really a failure of pcr in identifying parasitologically positive tsetse flies in the field. tsetse flies (glossina palpalis gambiensis and glossina morsitans morsitans) were therefore experimentally infected with two different species of trypanosoma (trypanosoma brucei gambiense or trypanosoma congolense). a total of 152 tsetse flies were dissected, and organs of each fly (midgut, proboscis or salivary glands) were examined. ... | 2004 | 15071836 |
| monitoring the susceptibility of glossina palpalis gambiensis and g. morsitans morsitans to experimental infection with savannah-type trypanosoma congolense, using the polymerase chain reaction. | teneral glossina palpalis gambiensis and g. morsitans morsitans (diptera: glossinidae) were fed on mice infected with savannah-type trypanosoma (nannomonas) congolense. the infection was monitored by checking the post-feeding diuresis fluid (midgut infection) and saliva (mature infection) of individual flies for parasites, at different times post-infection, using microscopical examination and a pcr-based assay. the results indicated that both tsetse species supported established midgut infection ... | 2004 | 15000728 |
| experimental infection of n'dama cattle with trypanosomes using glossina palpalis gambiensis caught in the wild. | the transmissibility of trypanosome infection to n'dama cattle by tsetse flies caught in the field was examined. wild-caught glossina palpalis gambiensis were transferred singly into small numbered cages and allowed to feed on 14 uninfected n'dama cattle. following a completed feed the tsetse were dissected and infection in the proboscis, the salivary glands and the gut was recorded. each animal was bitten by a number of tsetse ranging from five up to 64 flies. following dissection of the tsetse ... | 1990 | 2321260 |