| oral immunization of monkeys with polyvalent dysentery vaccine. | macaca mulatta monkeys were immunized orally with polyvalent boivin extract of shigella flexneri 2a, 3a, 4a, 4b, 6 and shigella sonnei. the total immumizing dose for each component was equivalent to 1.2 x 10(12) cells. after challenge with 7.5 x 10(10) cells of a virulent s. flexneri 2a strain, out of 20 immunized animals 2 developed dysentery and 4 showed mild dyspepsia; all 6 control animals became ill with dysentery. vaccination failed to influence the incidence, duration or the intermittent ... | 1977 | 412396 |
| protective ribosomal preparation from shigella sonnei as a parenteral candidate vaccine. | a parenteral shigella ribosomal vaccine (srv) was investigated in animals for safety, antibody-inducing capacity, and protective activity. ribosomal preparations from a shigella sonnei phase i avirulent strain were obtained and shown to possess chemical, sedimentation, and other properties typical of bacterial ribosomes. no endotoxin contamination was revealed by a ketodeoxyoctonate assay, although the presence of some kind of o antigen was evidenced by serological findings and the high activity ... | 1991 | 1716612 |
| construction, characterization, and animal testing of wrsd1, a shigella dysenteriae 1 vaccine. | wrsd1 is a shigella dysenteriae 1 vaccine containing deletions of the virg(icsa) gene required for intercellular spreading and a 20-kb chromosomal region encompassing the shiga toxin genes (stxab). wrsd1 was constructed from s. dysenteriae 1 strain 1617 that was originally isolated during the 1968 to 1969 epidemic of shiga dysentery in guatemala. the virg(icsa) deletion was constructed from a streptomycin-resistant (str(r)) mutant of 1617 by a filter mating procedures using a virg(icsa) deletion ... | 2002 | 12010984 |
| construction of a trivalent candidate vaccine against shigella species with dna recombination. | in this work asd gene of shigella flexneri 2a strain t32 was replaced by vibrio cholerae toxin b subunit (ctxb) gene with dna recombination in vivo and in vitro. the resulting derivative of t32, designed as fwl01, could stably express ctxb, but its growth in lb medium depended on the presence of diaminopimelic acid (dap). then form i plasmid of shigella sonnei strain s7 was labeled with strain t32 asd gene and mobilized into fwl01. thus a trivalent candidate oral vaccine strain, designed as fsw0 ... | 2002 | 18763059 |
| safety and colonization of two novel virg(icsa)-based live shigella sonnei vaccine strains in rhesus macaques (macaca mulatta). | shigella are gram-negative bacterium that cause bacillary dysentery (shigellosis). symptoms include diarrhea and discharge of bloody mucoid stools, accompanied by severe abdominal pain, nausea, vomiting, malaise, and fever. persons traveling to regions with poor sanitation and crowded conditions become particularly susceptible to shigellosis. currently a vaccine for shigella has not been licensed in the united states, and the organism quickly becomes resistant to medications. during the past 10 ... | 2008 | 19793462 |
| shigella sonnei vaccine candidates wrss2 and wrss3 are as immunogenic as wrss1, a clinically tested vaccine candidate, in a primate model of infection. | shigella causes diarrhea and dysentery through contaminated food and water. shigella sonnei live vaccine candidates wrss2 and wrss3 are attenuated principally by the loss of virg(icsa) that prevents bacterial spread within the colonic epithelium. in this respect they are similar to the clinically tested vaccine candidate wrss1. however, wrss2 and wrss3 are further attenuated by loss of sena, senb and wrss3 also lacks msbb2. as previously shown in cell culture assays and in small animal models, t ... | 2011 | 21596086 |
| oral administration of live shigella vaccine candidates in rhesus monkeys show no evidence of competition for colonization and immunogenicity between different serotypes. | live oral monovalent shigella flexneri 2a vaccine candidates as well as bivalent formulations with shigella sonnei were evaluated in a rhesus monkey model for colonization and immunogenicity. freshly harvested suspensions of s. flexneri 2a vaccine candidates wrsf2g12 and wrsf2g15 as well as s. sonnei vaccine candidate wrss3 were nasogastrically administered to groups of rhesus monkeys, macaca mulatta, either in a monovalent form or when combined with each other. the animals were monitored daily ... | 2014 | 24522159 |