west nile virus circulation in mosquitoes in greece (2010-2013).background of the study. following a large west nile virus (wnv) epidemic in northern greece in 2010, an active mosquito surveillance system was implemented, for a 3-year period (2011, 2012, and 2013). description of the study site and methodology. using mainly co2 mosquito traps, mosquito collections were performed. samples were pooled by date of collection, location, and species and examined for the presence of wnv. results. positive pools were detected in different areas of the country. in 20 ...201627294111
west nile virus in mosquitoes in greece.epidemics of west nile virus (wnv) occurred for two consecutive years in greece (in 2010 and 2011). a total of 16,116 adult culex pipiens mosquitoes collected in two peripheries, central macedonia and thessaly, were tested for wnv infection. wnv lineage 2 was detected in 6/296 mosquito pools, three in each year. the h249p substitution in the ns3 protein, previously associated with increased pathogenicity and thermotolerance, was detected in all six wnv-positive mosquito pools. when 21 individual ...201323371497
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