| detection of rickettsia japonica in haemaphysalis longicornis ticks by restriction fragment length polymorphism of pcr product. | pcr was applied to the detection of rickettsia japonica, the causative agent of oriental spotted fever (osf), in ticks collected at two sites of the muroto area on shikoku island, a major area in japan where osf is endemic. primer pair rr190.70p and rr190.602n of the r. rickettsii 190-kda antigen gene sequence of regnery and others (r.l. regnery, c.l. spruill, and b.d. plikaytis, j. bacteriol. 173:1576-1589, 1991) primed the dna extracted from haemaphysalis longicornis ticks but not those extrac ... | 1995 | 7790445 |
| [spotted fever group rickettsiosis and vectors in kanagawa prefecture]. | primer pairs for pcr were designed from the gene encoding the 17,000-molecular-weight genus-common antigen of rickettsia japonica, rickettsia rickettsii, rickettsia conorii, rickettsia typhi and rickettsia prowazekii. primers r1, r2 were designed for amplifying the genomic dna from spotted fever group (sfg) rickettsiae and epidemic typhus rickettsiae. primers rj5, rj10 were designed for amplifying the genomic dna from only r. japonica. using the primers r1, r2, about a 540-bp fragment was observ ... | 1996 | 8741704 |
| genetic identification of rickettsiae isolated from ticks in japan. | following the description in japan of japanese spotted fever, caused by rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by pcr and monoclonal antibody testing. we identified these six strains by amplification and sequencing of the genes encoding 16s rrna and citrate synthase. we confirmed that the i ... | 2002 | 12037083 |
| ectoparasites of the pallas squirrel, callosciurus erythraeus, introduced to japan. | the squirrel callosciurus erythraeus (pallas) (rodentia: sciuridae) was intentionally introduced to japan in 1935 and has become established throughout much of the country. although they live mainly in forests, pallas squirrels come into gardens and are frequently fed by people or kept as pets, so their ectoparasites could be of potential medical as well as veterinary importance. during 2001-2003 we conducted the first ectoparasite survey of pallas squirrels in japan. from 105 c. erythraeus capt ... | 2004 | 15009447 |