| environmental transmission of the gut symbiont burkholderia to phloem-feeding blissus insularis. | the plant-phloem-feeding blissus insularis possesses specialized midgut crypts, which harbor a dense population of the exocellular bacterial symbiont burkholderia. most individual b. insularis harbor a single burkholderia ribotype in their midgut crypts; however, a diverse burkholderia community exists within a host population. to understand the mechanism underlying the consistent occurrence of various burkholderia in b. insularis and their specific association, we investigated potential gut sym ... | 2016 | 27548682 |
| culturing and characterization of gut symbiont burkholderia spp. from the southern chinch bug, blissus insularis (hemiptera: blissidae). | the phloem-feeding southern chinch bug, blissus insularis, harbors a high density of the exocellular bacterial symbiont burkholderia in the lumen of specialized midgut crypts. here we developed an organ culture method that initially involved incubating the b. insularis crypts in osmotically balanced insect cell culture medium. this approach enabled the crypt-inhabiting burkholderia spp. to make a transition to an in vitro environment and to be subsequently cultured in standard bacteriological me ... | 2016 | 27016568 |
| a synchronous rearing method for blissus insularis (hemiptera: blissidae). | the southern chinch bug, blissus insularis barber (hemiptera: blissidae), is the most destructive insect pest of st. augustinegrass, stenotaphrum secundatum (walt.) kuntze. management of b. insularis has depended on frequent insecticide applications, which has resulted in populations becoming resistant to several insecticide classes. to facilitate developing a resistance management program for this pest, it is necessary to develop methods to rear insects of known age, generation, and pesticide e ... | 2010 | 20568618 |
| impacts of antibiotic and bacteriophage treatments on the gut-symbiont-associated blissus insularis (hemiptera: blissidae). | the southern chinch bug, blissus insularis, possesses specialized midgut crypts that harbor dense populations of the exocellular symbiont burkholderia. oral administration of antibiotics suppressed the gut symbionts in b. insularis and negatively impacted insect host fitness, as reflected by retarded development, smaller body size, and higher susceptibility to an insecticide, bifenthrin. considering that the antibiotics probably had non-lethal but toxic effects on host fitness, attempts were con ... | 2016 | 27827869 |
| differential probing behavior of blissus insularis (hemiptera: blissidae) on resistant and susceptible st. augustinegrasses. | southern chinch bug, blissus insularis barber, is a severe pest of st. augustinegrass throughout the southern united states. host plant resistance is an environmentally friendly method to manage chinch bug infestations and is increasingly important, as the southern chinch bug develops resistance to insecticides. in this study, in an effort to understand resistance mechanisms in two varieties of st. augustinegrass ('fx-10' and 'nuf-76'), we used the electrical penetration graph method to quantify ... | 2015 | 26470190 |
| st. augustine grass germplasm resistant to blissus insularis (hemiptera: blissidae). | st. augustine grass (stenotaphrum secundatum (walter) kuntze) is an economically important turfgrass in the southeastern united states. however, this turf species is prone to southern chinch bug, blissus insularis barber (heteroptera: blissidae) outbreaks. this insect is the most destructive pest of st. augustine grass wherever this turf grass is grown. host plant resistance has historically been an effective management tool for southern chinch bug. since 1973, the 'floratam' st. augustine grass ... | 2014 | 25195463 |
| detection and characterization of bacterial symbionts in the heteropteran, blissus insularis. | dense populations of extracellular bacteria were detected in midgut crypts of the southern chinch bug, blissus insularis barber (hemiptera: blissidae). examination by epifluorescent and transmission electron microscopy revealed that the bacteria covered the luminal surface of the crypts and filled the entire lumen. attempts to culture the extracellular endosymbionts in various media failed. sequencing and phylogenetic analyses of 16s rrna gene clones obtained from insects of five florida populat ... | 2012 | 22713107 |
| genetic variability in populations of the southern chinch bug, blissus insularis, assessed using aflp analysis. | southern chinch bug, blissus insularis barber (heteroptera: blissidae), is the most destructive insect pest of st. augustine grass, stenotaphrum secundatum waltz (kuntze), in the southern united states. the present study is focused on assessing genetic variability in five populations of b. insularis collected from texas and florida where st. augustine grass is widely grown. the amplified fragment length polymorphism technique was used to dna fingerprint individuals from each population (a total ... | 2011 | 22957773 |
| oxidative responses of st. augustinegrasses to feeding of southern chinch bug, blissus insularis barber. | southern chinch bug, blissus insularis barber (hemiptera: blissidae), is a serious insect pest of st. augustinegrass, stenotaphrum secundatum (walt.) kuntze, a turfgrass commonly grown in the southeastern united states. resistance to southern chinch bug has been identified in the polyploid st. augustinegrass varieties 'floratam' and 'fx-10', and the diploid 'captiva'. however, southern chinch bug in florida and elsewhere has overcome floratam's resistance. this research investigated the potentia ... | 2009 | 19588198 |
| role of leaf sheath lignification and anatomy in resistance against southern chinch bug (hemiptera: blissidae) in st. augustinegrass. | southern chinch bug, blissus insularis barber (hemiptera: blissidae), is the most serious insect pest of st. augustinegrass stenotaphrum secundatum (walter) kuntze, a common lawngrass grown in southeastern u.s. states. host plant resistance to southern chinch bug has been identified in the polyploid st. augustinegrass 'fx-10' and the diploid 'captiva'. the objective of this research was to identify possible physical mechanism(s) explaining chinch bug resistance in these cultivars. we studied the ... | 2009 | 19253665 |
| categories of resistance in st. augustinegrass lines to southern chinch bug (hemiptera: blissidae). | the southern chinch bug, blissus insularis barber (hemiptera: blissidae), is the most important insect pest of st. augustinegrass, stenotaphrum secundatum (walt.) kuntze, in florida and other gulf coast states. resistance to southern chinch bug was identified previously in st. augustinegrass lines 'fx-10' and nuf-76. choice and no-choice tests and ovipositional and developmental studies were conducted to determine the categories of resistance in fx-10 and nuf-76 to southern chinch bug. when adul ... | 2006 | 16937703 |
| evaluation of cool- and warm-season grasses for resistance to multiple chinch bug (hemiptera: blissidae) species. | chinch bugs are common pests of many agronomic and horticulturally important crops and turfgrasses. the extensive overlap of plant hosts and geographic distribution of blissus leucopterus leucopterus (say), blissus leucopterus hirtus montandon, blissus insularis barber, and blissus occiduus barber underscores the importance of identifying resistant germplasm. cool- and warm-season turfgrasses and sorghum, sorghum bicolor (l.) moench, were evaluated for resistance to chinch bugs in the blissus co ... | 2006 | 16573341 |