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pcr detection of babesia ovata from questing ticks in japan.babesia ovata is a tick-transmitted hemoprotozoan parasite of cattle. in the present study, we analyzed tick dna samples (n=1459) prepared from questing ticks collected from various cattle pastures in hokkaido (shibecha, taiki, otofuke, memuro, and shin-hidaka districts) and okinawa (yonaguni island) prefectures of japan for b. ovata. when all the tick dna samples were screened by a previously described b. ovata-specific apical membrane antigen-1 (ama-1) gene-based polymerase chain reaction (pcr ...201424572609
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