Publications

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the incrimination of aedes (stegomyia) aegypti as the vector of dirofilaria repens in nigeria.six local species of culicides were identified as the common mosquitoes in zaria, out of 15 species captured using various adult and larval collection methods. these common culicides are culex pipiens fatigans, anopheles gambiae grp., mansonia africana, culex pipiens pipiens, aedes (stegomyia) aegypti and aedes vittatus. they were each fed directly on a local dog naturally infected with dirofilaria repens to evaluate their refractoriness/susceptibility to dirofilarial infection. in a number of d ...200010996744
aedes albopictus and culex pipiens implicated as natural vectors of dirofilaria repens in central italy.to identify the natural vectors of dirofilaria repens railliet et henry, entomological samplings were carried out in four sites within the lazio region, foci of canine subcutaneous dirofilariasis. collections were made in 2002-2003 by means of dog-baited and miniature centers for disease control and prevention traps as well as on humans. microscopy identified 1576 attracted mosquito females as belonging to six species, but molecular diagnostics detected filarial dna only in culex pipiens l. and ...200718047207
a duplex real-time polymerase chain reaction assay for the detection of and differentiation between dirofilaria immitis and dirofilaria repens in dogs and mosquitoes.this study describes a duplex real-time polymerase chain reaction (pcr) assay for the detection and differentiation between dirofilaria immitis and dirofilaria repens in dog blood and mosquitoes. regions of a cytochrome oxidase 1 (cox1) mitochondrial dna fragment and the second internal transcribed spacer (its-2) of nuclear ribosomal dna were amplified from microfilariae and adult worm samples, using a sensitive ssofast™ evagreen(®) based real-time pcr method coupled with melting-curve analysis. ...201122119343
mosquitoes (diptera: culicidae) and their relevance as disease vectors in the city of vienna, austria.mosquitoes (diptera: culicidae) are important vectors for a wide range of pathogenic organisms. as large parts of the human population in developed countries live in cities, the occurrence of vector-borne diseases in urban areas is of particular interest for epidemiologists and public health authorities. in this study, we investigated the mosquito occurrence in the city of vienna, austria, in order to estimate the risk of transmission of mosquito-borne diseases. mosquitoes were captured using di ...201425468380
dirofilaria repens microfilariae in aedes vexans mosquitoes in slovakia.in this study, we screened field-caught mosquitoes for presence of dirofilaria spp. by using a polymerase chain reaction (pcr) assay. potential occurrence of dirofilaria repens and dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (aedes vexans, aedes cinereus, aedes rossicus, culex pipiens, culiseta annulata, ochlerotatus sticticus, ochlerotatus cantans and ochlerotatus caspius) collected from five locations in two districts (kosice and trebisov) of eastern slo ...201323846240
molecular survey of dirofilaria immitis and dirofilaria repens by direct pcr for wild caught mosquitoes in the republic of korea.adult mosquito collections using new jersey light traps and black-hole light traps were conducted to determine the potential vectors and the relative mosquito infection rates of dirofilaria immitis and dirofilaria repens in gyeonggi and gangwon provinces, republic of korea, 2005. dirofilaria spp. were confirmed by polymerase chain reaction (pcr) using species-specific primers for d. immitis and d. repens. minimum field infection rates (mfir) [mfir = (number infected pools/total number of mosquit ...200717644255
natural vectors of dirofilariasis in rural and urban areas of the tuscan region, central italy.entomological investigations by means of dog- and human-baited traps were carried out in summers 2000-2002 in urban and rural areas of the tuscan region in central italy. the aim of the study was to define the mosquito species involved in the transmission of dirofilaria nematodes and to assess the risk that their presence might represent for animal and human health. nocturnal fieldwork on host-seeking activity and feeding preferences was followed by microscopic identification of the mosquito spe ...200616739418
[bloodsucking mosquitoes (diptera: culicidae) in, the tula region are potential vectors of dirofilarias].bloodsucking mosquitoes were collected in tula and its region in may to august 2013-2014. the fauna included 17 species from 5 genera in the subfamily culicinae and anopheles maculipennis complex in the subsystem anophelinae. ochlerotatus cantans was a dominant species in the collections. the dominant species also included aedes einereus, ae. vexans, ae. geniculatus, och. diantaeus, och. intrudens, och. cataphylla, and culex pipiens. the possible value of different mosquito species dirofilaria r ...201626827580
canine dirofilariosis endemic in central europe-10 years of epidemiological study in slovakia.the study presents the comprehensive results of a detailed epidemiological study on canine dirofilariosis in slovakia, central europe. more than 4000 dogs were investigated, and several epidemiological factors were considered. the mean prevalence in individual regions ranged from 2.0 % in northern slovakia to more than 25.0 % in the south-western part of the country, with the nematode dirofilaria repens confirmed as the dominant causative agent. canine dirofilariosis occurred more often in anima ...201627021185
potential mosquito (diptera:culicidae) vector of dirofilaria repens and dirofilaria immitis in urban areas of eastern slovakia.this paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of dirofilaria spp. which provided the information on aedes vexans as a potential vector of dirofilaria repens in slovakia. current entomological and molecular research indicates that ae. vexans can participate also in the transmission of dirofilaria immitis within the region. using the standard pcr method, we examined 10,500 mosquitoes (ae. vexans, ae. rossicus, anopheles maculipennis s.l., ochler ...201526391170
detection of dirofilaria repens and dirofilaria immitis dna in mosquitoes from belarus.during the last two decades, belarus faces an increase of human cases of dirofilaria (nematoda, spirurida, onchocercidae) infections. however, comprehensive analyses explaining this development and the identification of mosquito vector species are missing. here, we present results using temperature data from belarus and show that the annual number of human dirofilaria cases is significantly correlated with the yearly average temperatures (spearman's rho = 0.49, p < 0.05) and the average sum of p ...201627169723
prevalence of dirofilaria immitis (nematoda: filarioidea) in mosquitoes from northeast arkansas, the united states.a mosquito survey was conducted to identify which species of mosquitoes carry dirofilaria immitis (leidy) (nematoda: filarioidea), dog heartworm, in northeast arkansas. using polymerase chain reaction, mosquitoes were analyzed for d. immitis, dirofilaria repens railliet & henry, and acanthocheilonema dracunculoides cobbold. mosquitoes were collected from april to october 2009 using black light ultraviolet traps baited with dry ice. sixteen mosquito species were identified. d. immitis was identif ...201323926787
molecular detection of dirofilaria immitis, dirofilaria repens and setaria tundra in mosquitoes from germany.as a result of globalization and climate change, dirofilaria immitis and dirofilaria repens, the causative agents of dirofilariosis in europe, continue to spread from endemic areas in the mediterranean to northern and northeastern regions of europe where autochthonous cases of dirofilarial infections have increasingly been observed in dogs and humans. whilst d. repens was recently reported from mosquitoes in putatively non-endemic areas, d. immitis has never been demonstrated in mosquitoes from ...201424433279
molecular xenomonitoring of dirofilaria immitis and dirofilaria repens in mosquitoes from north-eastern italy by real-time pcr coupled with melting curve analysis.dirofilaria immitis and dirofilaria repens are transmitted by bloodsucking culicid mosquitoes belonging to culex, aedes, ochlerotatus, anopheles and mansonia genera. the detection of filarioids in mosquitoes for assessing distribution of vectors and/or of pathogens in a given area (also known as "xenomonitoring"), when based on individual dissection of wild-caught female mosquitoes is time consuming and hardly applicable in large epidemiological surveys. our study aimed to evaluate the recently ...201222520170
dirofilaria repens railliet et henry, 1911--a new parasite acclimatized in poland.dirofilariosis caused by the dirofilaria repens nematodes is widely dispersed in southern europe, asia and africa among dogs, cats, other carnivores and occasionally, humans. the first case of d. repens infection, found in poland in 2007, concerned humans. in 2009, dirofilariosis was first registered in dogs in 3 focuses in central poland, warsaw, pruszków and zyrardów, whose range grew considerably with the subsequent identification of d. repens microfilariae in 119 dogs in warsaw and 18 distri ...201424930244
first molecular identification of dirofilaria spp. (onchocercidae) in mosquitoes from serbia.dirofilariosis is a common and widespread veterinary health issue in several european countries with notable zoonotic potential. the causative agents are dirofilaria immitis and dirofilaria repens nematoda species which are transmitted by different mosquito vectors. similar to other mosquito-borne infections, the knowledge about mosquito species involved in disease transmission is crucial for the complex understanding of local transmission cycles. since there is no available data on mosquito spe ...201627193348
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