| ultrastructure of babesia major vermicules from the tick haemaphysalis punctata as demonstrated by negative staining. | the ultrastructure of babesia major vermicules was studied in samples derived from the haemolymph of haemaphysalis punctata adults and negatively stained with phosphotungstic acid. most of the organelles observed were typical of those found in apicomplexan parasites. these were the apical complex with the polar ring and the ribs, micronemes and subpellicular microtubules. the number of ribs was 27 or 28. the outer membrane of the pellicle was composed of a large number of fibrils running along t ... | 1978 | 77093 |
| the ultrastructure of penetrating stages of babesia major infecting the ovary of haemaphysalis punctata. | the ultrastructure of penetrating stages of babesia major merozoites responsible for causing trans-ovarian infection of the tick haemaphysalis punctata was studied. these merozoites were different from forms of other babesia species previously described in the tick in that they were highly pleomorphic. most of the organelles found in these forms were similar to those found in other stages: however, the apical complex was found to be very active. micronemes were found to be predominant in the cyt ... | 1977 | 563562 |
| babesia major in britain: infectivity for cattle of cryopreserved parasites derived from haemaphysalis punctata nymphs. | babesia major-infected haemaphysalis punctata nymphs, prefed for four days on rabbits, were ground in a modified eagle's minimum essential medium containing n-2 hydroxyethylpiperazine-n1-ethane-sulphonic acid (hepes) buffer using sterile sand or ground glass as triturating agents. the resultant supernatant fluids were shown to be infective for splenectomised calves. when attempts were made to cryopreserve the fluids using either dimethyl sulphoxide or glycerol to a final concentration of 10 per ... | 1977 | 860093 |
| a differential diagnostic criterion for babesia major and babesia bigemina vermicules from tick haemolymph. | babesia major mature and immature vermicules in the haemolymph of haemaphysalis punctata were measured and found to be significantly larger than vermicules of babesia bigemina. mature b. major vermicules had a mean length of 15.53 micrometer and mature b. bigemina vermicules had a mean length of 11.79 micrometer. this difference provides a new criterion for the differentiation of the two species. | 1977 | 906629 |
| experimental transmission of babesia major by haemaphysalis punctata. | using a newly isolated strain of babesia major and a clean strain of laboratory reared haemaphysalis punctata it was shown that adult female ticks could be alimentarily infected by feeding on infected calves but that larvae and nymphae could not. all stages were able to transmit the parasite. two syringe passages of b major resulted in a complete loss of infectivity to ticks. | 1977 | 928992 |
| ultrastructure of babesia major in the tick haemaphysalis punctata. | haemaphysalis punctata ticks were infected with babesia major by allowing them to feed on an infected splenectomised calf. heavily infected ticks were dissected and the guts and the ovaries were processed to study the ultrastructure of b major in these organs. the morphology of the parasites in the gut and the ovary was identical. it was shown that the parasite contained a reduced form of an apical complex consisting of characteristic organelles such as the polar ring, rhoptries, micronemes and ... | 1976 | 951514 |
| [occurrence of the tick haemaphysalis punctata (can. et fanz., 1877) and of babesia major in cattle in north germany]. | | 1976 | 1008781 |
| experiments on the transmission of babesia major and babesia bigemina by haemaphysalis punctata. | experiments on the transmission by haemaphysalis punctata of three large babesia strains were carried out. three babesia-free batches of laboratory reared h. punctata ticks were infected with two strains of babesia major, b. major (xingjiang strain), isolated with adult ticks of h punctata and b. major (henan strain), isolated with h. longicornis) and a strain of babesia bigemina by feeding them on the calves infected by inoculation of blood stabilates. h. punctata was shown to be capable of tra ... | 1996 | 9011018 |