| rapid identification and differentiation of fasciola hepatica and fasciola gigantica by a loop-mediated isothermal amplification (lamp) assay. | the present study developed and validated a species-specific loop-mediated isothermal amplification (lamp) assay for the rapid detection and discrimination of fasciola hepatica and fasciola gigantica. the lamp assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 °c or 62 °c by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. the opti ... | 2010 | 20933335 |
| dermatitis caused by cercariae of orientobilharzia turkestanicum in the caspian sea area of iran. | cercarial dermatitis is very common among rice farmers in the caspian sea area of iran. the characteristic eggs of orientobilharzia turkestanicum were found in the feces of a plowing bull. fifty-six percent of uninfected lymnaea gedrosiana maintained at 24-25 degrees c produced cercariae, identical to those from natural infections, 21 days after exposure to the miracidia obtained from the feces of the bull. cercariae of o. turkestanicum from naturally infected l. gedrosiana produced dermatitis o ... | 1979 | 484774 |
| orientobilharzia species: neglected parasitic zoonotic agents. | parasites of the genus orientobilharzia belong to platyhelminthes, trematoda, digenea, schistosomatidae, and the type species is orientobilharzia turkestanicum. o. turkestanicum was first described by skrjabin from cattle in russian turkestan in 1913. adult worms of orientobilharzia species live in the portal veins or intestinal veins of cattle, sheep and other mammals, and often cause orientobilharziasis in china, india, mongolia, pakistan, iraq, iran in asia, and russia and turkey in europe. m ... | 2009 | 19081387 |
| the phylogeography of asian schistosoma (trematoda: schistosomatidae). | partial (dna) sequences are presented for 2 nuclear (18s and 28s rrna genes) and 2 mitochondrial (12s rrna and nd1 genes) loci for 5 species belonging to the schistosoma japonicum, s. sinensium and s. indicum groups of asian schistosoma. fresh field isolates were collected and cultured for the following taxa: s. incognitum (s. indicum group, central thailand), s. mekongi (s. japonicum group, southern laos), s. ovuncatum (s. sinensium group, northern thailand), s. spindale (s. indicum group, nort ... | 2002 | 12211613 |
| recognition of cattle skin by cercariae of orientobilharzia turkestanica. | the cercariae of orientobilharzia turkestanica attached to isolated cattle skin and penetrated it. the attachment was stimulated by warmth and by hydrophilic and lipophilic chemical cues of skin surface extracts. the enduring contact with the skin was also stimulated by the chemical cues, but not by warmth. penetration of the cercariae into agar substrates was triggered by skin surface lipids, and free fatty acids were identified as the exclusively active compounds of the lipids. that the cercar ... | 2001 | 11570554 |
| indian schistosomes: a need for further investigations. | india is uniquely positioned with regard to schistosomes and schistosomiasis-discovering seven new mammalian species with the existence of three more schistosome species: orientobilharzia turkestanicum, o. harinasutai, and schistosoma haematobium(?). an endemic focus of urinary schistosomiasis was reported from gimvi village of ratnagiri, maharashtra with infrequent occurrence of schistosome eggs in human stools. cercarial dermatitis has been reported to be more abundant in rural population usin ... | 2011 | 22132307 |
| histochemistry of the cercaria of orientobilharzia turkestanica (skrjabin, 1913). | four types of gland cells--escape, head, praeacetabular and postacetabular--were demonstrated by histochemical methods in the cercaria of orientobilharzia turkestanica. the secretion of the head gland contains proteins with cystine and phospholipids. it exhibits the acid phosphatase activity. the secretion of the escape glands contains proteins with cystine and cysteine and the secretion of the postacetabular gland cells proteins with cystine, cysteine and tyrosine, and neutral and acid mucosubs ... | 1986 | 3804083 |
| [its and 28s rdna-lsu sequence analysis of orientobilharzia turkestanicum from bovine and caprine hosts]. | to explore sequence differentiation of its and 28s rdna-lsu of orientobilharzia turkestanicum from bovine and caprine hosts. | 2008 | 19160963 |
| orientobilharzia turkestanicum is a member of schistosoma genus based on phylogenetic analysis using ribosomal dna sequences. | in the present study, samples representing orientobilharzia turkestanicum from cattle, sheep, cashmere goat and goat in heilongjiang province, china, were characterized and grouped genetically by sequences of internal transcribed spacer (its, including its-1 and its2) and 28s ribosomal dna (28s rdna). the its and 28s rdna were amplified by polymerase chain reaction (pcr) and then sequenced and compared with that of other members of the schistosomatidae available in genbank, and phylogenetic rela ... | 2009 | 19014940 |
| orientobilharzia turkestanicum is grouped within african schistosomes based on phylogenetic analyses using sequences of mitochondrial genes. | in the present study, samples representing orientobilharzia turkestanicum from cattle, sheep, and cashmere goat in daqing district, heilongjiang province, china, were characterized and grouped genetically by sequences of mitochondrial cytochrome c oxidase subunit 1 gene (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 gene (nad1). genomic dnas were extracted from parasites isolated from individual host by sodium dodecyl sulfate-protease k treatment. the cox1 and nad1 genes we ... | 2008 | 18176856 |
| [africa or asia, which is the evolutionary origin of human schistosomes?]. | the origin and the evolution of schistosomatidae species, due to their medical importance (responsible of the second most important human parasitosis after malaria), arouse a great interest. a combination of phylogenetic studies using several molecular markers has provided support for the traditional grouping and evolutionary inferences derived from morphological and biological data. the genus schistosoma, which comprises all species parasitizing man, is generally split into four evolutionary li ... | 2001 | 11725698 |
| de novo transcriptomic analysis of the female and male adults of the blood fluke schistosoma turkestanicum. | schistosoma turkestanicum is a parasite of considerable veterinary importance as an agent of animal schistosomiasis in many countries, including china. the s. turkestanicum cercariae can also infect humans, causing cercarial dermatitis in many countries and regions of the world. in spite of its significance as a pathogen of animals and humans, there is little transcriptomic and genomic data in the public databases. | 2016 | 26968659 |
| a genetically distinct schistosoma from radix luteola from nepal related to schistosoma turkestanicum: a phylogenetic study of schistosome and snail host. | during a survey of freshwater snails in the terai region of southern nepal, 16 of 2588 specimens of radix luteola from 4 different habitats were found to be shedding schistosome cercariae. none of the 1411 specimens of radix acuminata we collected were positive for schistosomes. analysis of 28s, cox1, 16s and 12s sequences indicated that all the r. luteola-derived schistosomes were genetically very similar to one another and, although unambiguously grouping most closely to the widespread asian s ... | 2016 | 27542535 |
| signatures of mito-nuclear discordance in schistosoma turkestanicum indicate a complex evolutionary history of emergence in europe. | high levels of molecular diversity were identified in mitochondrial cytochrome c oxidase (cox1) gene sequences of schistosoma turkestanicum from hungary. these cox1 sequences were all specific to hungary which contrasted with the low levels of diversity seen in the nuclear internal transcribed spacer region (its) sequences, the majority of which were shared between china and iran isolates. measures of within and between host molecular variation within s. turkestanicum showed there to be substant ... | 2017 | 28747240 |
| [a rare zoonosis in hungary: cercarial dermatitis caused by schistosoma turkestanicum blood-fluke]. | several trematodes that parasitize vertebrate animals utilize swimming aquatic larvae to infect the host percutaneously. the most important ones among these parasites are the blood-flukes of birds and mammals comprising species that are also zoonotic. within this latter group are species that cause the bilharziasis or schistosomiasis of inhabitants of the tropical countries, and other trematode species that are able to penetrate human skin, but do not develop to an adult form of the worm in the ... | 2016 | 27690621 |
| characterization of micrornas from orientobilharzia turkestanicum, a neglected blood fluke of human and animal health significance. | the neglected blood flukes orientobilharzia spp. belonging to the platyhelminthes, infect animals in a number of countries of the world, and cause cercarial dermatitis in humans, as well as significant diseases and even death in economically-important animals. micrornas (mirnas) are now considered to be a key mechanism of gene regulation. herein, we investigated the global mirna expression profile of adult o. turkestanicum using next-generation sequencing technology and real-time quantitative pc ... | 2012 | 23071694 |
| the complete mitochondrial genome of orientobilharzia turkestanicum supports its affinity with african schistosoma spp. | orientobilharzia turkestanicum is a blood fluke of many mammals and causes orientobilharziasis that is also a neglected parasitic zoonosis because the cercaria of o. turkestanicum can infect humans and cause cercarial dermatitis. the present study determined the complete sequence of mt genome of o. turkestanicum and revised its phylogenetic position based on mt gene content and arrangement. the complete mtdna sequence of o. turkestanicum was 14,755 bp in length, which is slightly larger than the ... | 2011 | 21930247 |
| survey of helminths in adult sheep in heilongjiang province, people's republic of china. | the prevalence of helminths in adult sheep was investigated in heilongjiang province, people's republic of china between january 1999 and september 2003. a total of 326 adult sheep representing local breeds (xingjiang fine wool sheep, dongbei fine wool sheep) as well as introduced breeds (merino and charollais) from representative geographical locations in heilongjiang province were slaughtered and examined for the presence of helminths. the worms were examined, counted and identified to species ... | 2006 | 16713098 |
| [sequence analysis of rdna-lsu gene of orientobilharzia turkestanicum from mainland of china]. | to classify the taxonomic status of o. cheni in relation to o. turkestanicum var. tuberculata from the mainland of china by comparing their nucleotide sequences of nuclear ribosomal partial large subunit gene (lsu). | 2002 | 12567566 |
| studies on snail hosts of orientobilharzia turkestanicum (skrjabin, 1913) dutt and srivastava, 1955 (schistosomatidae: trematoda) in india. | | 1973 | 4720423 |
| [modification of the liver phospholipid status of cattle by orientobilharzia turkestanica trematodes]. | the paper gives the data on a trematode influence onto the phospholipid (pl) contents in the liver of cows. it is shown, that the decrease of pl basic fraction concentrations, i.e. phosphatidilcholin (pc) by 20% and phosphotidilethanolamin (pe) by 26%, during the period of infection is accompanied by the increase of respective lysoforms, namely, lysopc by 240%, and lysope by 160%, and as well as minor forms, pl-diphosphotidilgleserol by 70%, sphingomielin by 47% and phosphatidilserin by 41%. it ... | 1996 | 8984448 |
| genetic identification of orientobilharzia turkestanicum from sheep isolates in iran. | adult worms of orientobilharzia turkestanicum live in the portal veins, or intestinal veins of cattle, sheep, goat and many other mammals causing orientobilharziasis. orientobilharziasis causes significant economic losses to livestock industry of iran. however, there is limited information about genotypes of o. turkestanicum in iran. | 2017 | 25904947 |
| mitochondrial gene order change in schistosoma (platyhelminthes: digenea: schistosomatidae). | in the flatworm genus schistosoma, species of which include parasites of biomedical and veterinary importance, mitochondrial gene order is radically different in some species. a pcr-based survey of 19 schistosomatid spp. established which of 14 schistosoma spp. have the ancestral (plesiomorphic) or derived gene order condition. a phylogeny for schistosoma was estimated and used to infer the origin of the gene order change which is present in all members of a clade containing schistosoma incognit ... | 2012 | 23362512 |
| a foreign invader or a reclusive native? dna bar coding reveals a distinct european lineage of the zoonotic parasite schistosoma turkestanicum (syn. orientobilharzia turkestanicum ()). | natural foci of schistosoma turkestanicum (syn. orientobilharzia turkestanicum) has been identified in the gemenc forest regions of hungary utilising red deer as the definitive host. in order to identify the origins of this parasite in europe standard dna bar coding techniques were employed to sequence fragments of the cytochrome oxidase 1 (cox1) and the nuclear ribosomal internal transcribed region (its) from 10 individual adult male worms. phylogenetic reconstruction using maximum likelihood p ... | 2013 | 23220360 |
| [fatty acid composition of the liver phospholipids in the cow bos taurus infected with the trematode orientobilharzia turkestanica]. | | 2000 | 10752154 |
| a natural focus of the blood fluke orientobilharzia turkestanica (skrjabin, 1913) (trematoda: schistosomatidae) in red deer (cervus elaphus) in hungary. | the large american liver fluke, fascioloides magna - introduced to europe before 1875 - was the first non-indigenous trematode to be detected in hungarian cervids in 1995. as the most precious deer population became infected in the gemenc game reserve a study has been launched to assess the extent and the effect of f. magna infections and to examine the options for the treatment of infected deer populations. livers of red deer shot during regular hunting were submitted for the survey and were in ... | 2010 | 20347227 |