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Person-to-Person Transmission of Severe Fever with Thrombocytopenia Syndrome Virus.Abstract Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a newly discovered bunyavirus, SFTS virus (SFTSV), and causes high fatality (12% on average and as high as 30%). The objective of this study was to determine whether SFTSV could be transmitted from person to person. We analyzed sera of 13 patients from two clusters of unknown infectious diseases that occurred between September and November of 2006 in Anhui Province of China for SFTSV antibod ...201121955213
evolutionary and molecular analysis of the emergent severe fever with thrombocytopenia syndrome virus.in 2009, a novel bunyavirus, called severe fever with thrombocytopenia syndrome virus (sftsv) was identified in the vicinity of huaiyangshan, china. clinical symptoms of this zoonotic virus included severe fever, thrombocytopenia, and leukocytopenia, with a mortality rate of ~10%. by the end of 2011 the disease associated with this pathogen had been reported from eleven chinese provinces and human-to-human transmission suspected. however, current understanding of the evolution and molecular epid ...201223438426
detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization.a virus known as severe fever with thrombocytopenia syndrome virus (sftsv) was recently identified as the etiological agent of severe fever with thrombocytopenia syndrome (sfts) in china. reliable laboratory detection and identification of this virus are likely to become clinically and epidemiologically desirable. we developed a nearly instrument-free, simple molecular method which incorporates reverse transcription-cross-priming amplification (rt-cpa) coupled with a vertical flow (vf) visualiza ...201222993179
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes.viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ...201324314645
cytokine and chemokine levels in patients with severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv), which can cause hemorrhagic fever-like illness, is a newly discovered bunyavirus in china. the pathogenesis of sftsv infection is poorly understood. however, it has been suggested that immune mechanisms, including cytokines and chemokines, play an important role in disease pathogenesis. in the present study, we investigated host cytokine and chemokine profiles in serum samples of patients with sftsv infection from northeast china and exp ...201222911786
structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion.an emergent viral pathogen termed severe fever with thrombocytopenia syndrome virus (sftsv) is responsible for thousands of clinical cases and associated fatalities in china, japan, and south korea. akin to other phleboviruses, sftsv relies on a viral glycoprotein, gc, to catalyze the merger of endosomal host and viral membranes during cell entry. here, we describe the postfusion structure of sftsv gc, revealing that the molecular transformations the phleboviral gc undergoes upon host cell entry ...201627325770
the role of phlebovirus glycoproteins in viral entry, assembly and release.bunyaviruses are enveloped viruses with a tripartite rna genome that can pose a serious threat to animal and human health. members of the phlebovirus genus of the family bunyaviridae are transmitted by mosquitos and ticks to humans and include highly pathogenic agents like rift valley fever virus (rvfv) and severe fever with thrombocytopenia syndrome virus (sftsv) as well as viruses that do not cause disease in humans, like uukuniemi virus (uukv). phleboviruses and other bunyaviruses use their e ...201627455305
efficacy of t-705 (favipiravir) in the treatment of infections with lethal severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) is the causative agent of sfts, an emerging hemorrhagic fever. this disease has a high case fatality rate and is endemic to china, south korea, and japan. because there are currently no effective therapeutics for sfts, potent and safe antivirals are needed for the treatment of sfts. the inhibitory effect of t-705 (favipiravir) on the replication of sftsv in vero cells was evaluated. mice lacking the type i interferon receptor (ifnar(-/-)) ...201627303697
development of a colloidal gold kit for the diagnosis of severe fever with thrombocytopenia syndrome virus infection.it is critical to develop a cost-effective detection kit for rapid diagnosis and on-site detection of severe fever with thrombocytopenia syndrome virus (sftsv) infection. here, an immunochromatographic assay (ica) to detect sftsv infection is described. the ica uses gold nanoparticles coated with recombinant sftsv for the simultaneous detection of igg and igm antibodies to sftsv. the ica was developed and evaluated by using positive sera samples of sftsv infection (n = 245) collected from the cd ...201424826381
hijacking of rig-i signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type i interferon responses.recognition of viral pathogens by the retinoic acid-inducible gene i (rig-i)-like receptor (rlr) family results in the activation of type i interferon (ifn) responses. to avoid this response, most viruses have evolved strategies that target different essential steps in the activation of host innate immunity. in this study, we report that the nonstructural protein nss of the newly described severe fever with thrombocytopenia syndrome virus (sftsv) is a potent inhibitor of ifn responses. the sftsv ...201424478431
nonmuscle myosin heavy chain iia is a critical factor contributing to the efficiency of early infection of severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) is a novel phlebovirus in the bunyaviridae family. most patients infected by sftsv present with fever and thrombocytopenia, and up to 30% die due to multiple-organ dysfunction. the mechanisms by which sftsv enters multiple cell types are unknown. sftsv contains two species of envelope glycoproteins, gn (44.2 kda) and gc (56 kda), both of which are encoded by the m segment and are cleaved from a precursor polypeptide (about 116 kda) in the ...201324155382
genetic susceptibility is one of the determinants for severe fever with thrombocytopenia syndrome virus infection and fatal outcome: an epidemiological investigation.severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease in china and case-fatality rate of sfts is very high (approximately 10%). however, genetic susceptibility for sfts virus (sftsv) infection and fatal outcome of sftsv infection in humans are unclear. in this study, we investigated the clinical, laboratory and epidemiological features of sfts in a cluster of three sisters who died of sftsv infection between late april and mid-may 2014. before disease onset, two of ...201526207638
human antibody neutralizes severe fever with thrombocytopenia syndrome virus, an emerging hemorrhagic fever virus.severe fever with thrombocytopenia syndrome virus (sftsv), a newly discovered member of the bunyaviridae family, is the causative agent of an emerging hemorrhagic fever, sfts, in china. currently, there are no vaccines or effective therapies against sfts. in this study, a combinatorial human antibody library was constructed from the peripheral lymphocytes of 5 patients who had recovered from sfts. the library was screened against purified virions for the production of single-chain variable-regio ...201323863504
disruption of type i interferon signaling by the nonstructural protein of severe fever with thrombocytopenia syndrome virus via the hijacking of stat2 and stat1 into inclusion bodies.the type i interferon (ifn) system, including ifn induction and signaling, is the critical component of the host defense line against viral infection, which, in turn, is also a vulnerable target for viral immune evasion. severe fever with thrombocytopenia syndrome virus (sftsv) is an emerging bunyavirus. previous data have shown that sftsv can interfere with the early induction of type i ifns through targeting host kinases tbk1/ikkε. in this study, we demonstrated that sftsv also can suppress ty ...201525631085
experimental and natural infections of goats with severe fever with thrombocytopenia syndrome virus: evidence for ticks as viral vector.severe fever with thrombocytopenia syndrome virus (sftsv), the causative agent for the fatal life-threatening infectious disease, severe fever with thrombocytopenia syndrome (sfts), was first identified in the central and eastern regions of china. although the viral rna was detected in free-living and parasitic ticks, the vector for sftsv remains unsettled.201526485390
sfts virus in ticks in an endemic area of china.in total, 3,145 ticks of the species haemaphysalis longicornis (3,048; 96.9%), r. microplus (82; 2.6%), h. campanulata (9; 0.3%), and dermacentor sinicus (5; 0.2%) were collected from animals and vegetation at yantai in shandong province. both adult and immature ticks were obtained, and all ticks collected from vegetation were unfed. eggs were obtained from 22 blood-fed female ticks through maintenance at room temperature after collection. severe fever with thrombocytopenia syndrome virus (sftsv ...201525711611
first isolation of severe fever with thrombocytopenia syndrome virus from haemaphysalis longicornis ticks collected in severe fever with thrombocytopenia syndrome outbreak areas in the republic of korea.severe fever with thrombocytopenia syndrome (sfts) is an emerging tick-borne infectious disease that is endemic to china, japan, and the republic of korea (rok). in this study, 8313 ticks collected from sfts outbreak areas in the rok in 2013 were used to detect the sfts virus (sftsv). a single sftsv was isolated in cell culture from one pool of haemaphysalis longicornis ticks collected from samcheok-si, gangwon province, in the rok. phylogenetic analysis showed that the sftsv isolate was cluster ...201626745758
can we blame migratory birds for transmission of the emerging severe fever with thrombocytopenia syndrome virus in east asia? 026657298
severe fever with thrombocytopenia syndrome virus in ticks collected from humans, south korea, 2013.we investigated the infection rate for severe fever with thrombocytopenia syndrome virus (sftsv) among ticks collected from humans during may-october 2013 in south korea. haemaphysalis longicornis ticks have been considered the sftsv vector. however, we detected the virus in h. longicornis, amblyomma testudinarium, and ixodes nipponensis ticks, indicating additional potential sftsv vectors.025061851
no detection of severe fever with thrombocytopenia syndrome virus from ixodid ticks collected in seoul.larvae, nymphs, and adult stages of 3 species of ixodid ticks were collected by tick drag methods in seoul during june-october 2013, and their infection status with severe fever with thrombocytopenia syndrome (sfts) virus was examined using rt-pcr. during the period, 732 haemaphysalis longicornis, 62 haemaphysalis flava, and 2 ixodes nipponensis specimens were collected. among the specimens of h. longicornis, the number of female adults, male adults, nymphs, and larvae were 53, 11, 240, and 446, ...201424850970
characterization of the uukuniemi virus group (phlebovirus: bunyaviridae): evidence for seven distinct species.evolutionary insights into the phleboviruses are limited because of an imprecise classification scheme based on partial nucleotide sequences and scattered antigenic relationships. in this report, the serologic and phylogenetic relationships of the uukuniemi group viruses and their relationships with other recently characterized tick-borne phleboviruses are described using full-length genome sequences. we propose that the viruses currently included in the uukuniemi virus group be assigned to five ...201323283959
generation of mutant uukuniemi viruses lacking the nonstructural protein nss by reverse genetics indicates that nss is a weak interferon antagonist.uukuniemi virus (uukv) is a tick-borne member of the phlebovirus genus (family bunyaviridae) and has been widely used as a safe laboratory model to study aspects of bunyavirus replication. recently, a number of new tick-borne phleboviruses have been discovered, some of which, like severe fever with thrombocytopenia syndrome virus and heartland virus, are highly pathogenic in humans. uukv could now serve as a useful comparator to understand the molecular basis for the different pathogenicities of ...201525673721
reverse genetics system for severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) is an emerging tick-borne pathogen that was first reported in china in 2009. phylogenetic analysis of the viral genome showed that sfts virus represents a new lineage within the phlebovirus genus, distinct from the existing sandfly fever and uukuniemi virus groups, in the family bunyaviridae. sfts disease is characterized by gastrointestinal symptoms, chills, joint pain, myalgia, thrombocytopenia, leukocytopenia, and some hemorrhagic mani ...201425552716
phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in south korea and migratory bird routes between china, south korea, and japan.severe fever with thrombocytopenia syndrome (sfts) is a tick-borne viral disease. the sfts virus (sftsv) has been detected in the haemaphysalis longicornis, which acts as a transmission host between animals and humans. sftsv was first confirmed in china in 2009 and has also been circulating in japan and south korea. however, it is not known if a genetic connection exists between the viruses in these regions and, if so, how sftsv is transmitted across china, south korea, and japan. we therefore h ...201526033016
haemaphysalis longicornis ticks as reservoir and vector of severe fever with thrombocytopenia syndrome virus in china.severe fever with thrombocytopenia syndrome (sfts) is an emerging hemorrhagic fever in east asia caused by sfts virus (sftsv), a newly discovered phlebovirus. the haemaphysalis longicornis tick has been suspected to be the vector of sftsv. to determine whether sftsv can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of h. longicornis ticks and between ticks and mice. using reverse transcription pcr, we ...026402039
family cluster analysis of severe fever with thrombocytopenia syndrome virus infection in korea.severe fever with thrombocytopenia syndrome (sfts) is tick-borne viral disease that was first suspected in china in 2009. the causative virus (sftsv) was isolated in 2009 and reported in 2011, and sftsv expanded its geographic distribution in 2012-2013, from china to south korea and japan. most sftsv infections occur through haemaphysalis longicornis however, sftsv infection can also occur between family members, and nosocomial transmission of sftsv is also possible through close contact with a ...201627928083
spatial analysis of severe fever with thrombocytopenia syndrome virus in china using a geographically weighted logistic regression model.severe fever with thrombocytopenia syndrome (sfts) is caused by severe fever with thrombocytopenia syndrome virus (sftsv), which has had a serious impact on public health in parts of asia. there is no specific antiviral drug or vaccine for sftsv and, therefore, it is important to determine the factors that influence the occurrence of sftsv infections. this study aimed to explore the spatial associations between sftsv infections and several potential determinants, and to predict the high-risk are ...201627845737
severe fever with thrombocytopenia syndrome complicated by co-infection with spotted fever group rickettsiae, china.during 2013-2015 in central china, co-infection with spotted fever group rickettsiae was identified in 77 of 823 patients infected with severe fever with thrombocytopenia syndrome virus. co-infection resulted in delayed recovery and increased risk for death, prompting clinical practices in the region to consider co-infection in patients with severe fever with thrombocytopenia syndrome.027767921
severe fever with thrombocytopenia syndrome in patients suspected of having scrub typhus.to determine prevalence of severe fever with thrombocytopenia syndrome in south korea, we examined serum samples from patients with fever and insect bite history in scrub typhus-endemic areas. during the 2013 scrub typhus season, prevalence of this syndrome among patients suspected of having scrub typhus was high (23.0%), suggesting possible co-infection.027767909
accuracy of diagnosis of human granulocytic anaplasmosis in china.in 2008, human granulocytic anaplasmosis (hga) was reported from china. however, the clinical and laboratory findings, including reports of nosocomial transmission, were inconsistent with those reported for hga in the united states. in 2012, it was demonstrated that the patients described in the 2008 report had all been infected with a newly discovered bunyavirus, severe fever with thrombocytopenia syndrome virus, which causes an illness with the same clinical features described for the patients ...027648639
phleboviruses and the type i interferon response.the genus phlebovirus of the family bunyaviridae contains a number of emerging virus species which pose a threat to both human and animal health. most prominent members include rift valley fever virus (rvfv), sandfly fever naples virus (sfnv), sandfly fever sicilian virus (sfsv), toscana virus (tosv), punta toro virus (ptv), and the two new members severe fever with thrombocytopenia syndrome virus (sftsv) and heartland virus (hrtv). the nonstructural protein nss is well established as the main p ...201627338447
severe fever with thrombocytopenia syndrome virus antigen detection using monoclonal antibodies to the nucleocapsid protein.severe fever with thrombocytopenia syndrome (sfts) is a tick-borne infectious disease with a high case fatality rate, and is caused by the sfts virus (sftsv). sfts is endemic to china, south korea, and japan. the viral rna level in sera of patients with sfts is known to be strongly associated with outcomes. virological sfts diagnosis with high sensitivity and specificity are required in disease endemic areas.201627045364
seroepidemiological evidence of severe fever with thrombocytopenia syndrome virus infections in wild boars in nagasaki, japan.severe fever with thrombocytopenia syndrome (sfts) is an emerging disease in east asia. it is thought that the sfts virus (sftsv) circulates between ticks and animals in nature and that the virus is transmitted to humans by tick bites. sfts is endemic to nagasaki in western japan; however, epidemiological information regarding sftsv in nagasaki is not known. in this study, we performed sftsv igg elisas and neutralization antibody assays for a seroepidemiological survey using samples from wild bo ...201627433125
molecular evolution and spatial transmission of severe fever with thrombocytopenia syndrome virus based on complete genome sequences.severe fever with thrombocytopenia syndrome virus (sftsv) was a novel tick-borne bunyavirus that caused hemorrhagic fever with a high fatality rate in east asia. in this study we analyzed the complete genome sequences of 122 sftsv strains to determine the phylogeny, evolution and reassortment of the virus. we revealed that the evolutionary rate of three genome segments were different, with highest in the s segment and lowest in the l segment. the sftsv strains were phylogenetically classified in ...201626999664
phylogeographic analysis of severe fever with thrombocytopenia syndrome virus from zhoushan islands, china: implication for transmission across the ocean.from june 2011 to august 2014, 21 cases of infection by severe fever with thrombocytopenia syndrome bunyavirus (sftsv) were confirmed in zhoushan islands in the eastern coast of china. to identify the source of sftsv in zhoushan islands, the whole sftsv genomes were amplified and sequenced from 17 of 21 patients. the l, m, and s genomic segments of these sftsv strains were phylogenetically analyzed together with those of 188 sftsv strains available from genbank. phylogenetic analysis demonstrate ...201626806841
comprehensive molecular detection of tick-borne phleboviruses leads to the retrospective identification of taxonomically unassigned bunyaviruses and the discovery of a novel member of the genus phlebovirus.until the recent emergence of two human-pathogenic tick-borne phleboviruses (tbpvs) (severe fever with thrombocytopenia syndrome virus [sftsv] and heartland virus), tbpvs have been neglected as causative agents of human disease. in particular, no studies have addressed the global distribution of tbpvs, and consequently, our understanding of the mechanism(s) underlying their evolution and emergence remains poor. in order to provide a useful tool for the ecological and epidemiological study of tbp ...201425339769
the genome sequence of lone star virus, a highly divergent bunyavirus found in the amblyomma americanum tick.viruses in the family bunyaviridae infect a wide range of plant, insect, and animal hosts. tick-borne bunyaviruses in the phlebovirus genus, including severe fever with thrombocytopenia syndrome virus (sftsv) in china, heartland virus (hrtv) in the united states, and bhanja virus in eurasia and africa have been associated with acute febrile illness in humans. here we sought to characterize the growth characteristics and genome of lone star virus (lsv), an unclassified bunyavirus originally isola ...201323637969
risk assessment of human infection with a novel bunyavirus in china.to assess the public health risk of human infection from a novel bunyavirus - severe fever with thrombocytopenia syndrome virus (sftsv) - in china.201223908943
clinical features and factors associated with severity and fatality among patients with severe fever with thrombocytopenia syndrome bunyavirus infection in northeast china.in 2009, severe fever with thrombocytopenia syndrome virus (sftsv) was identified as a novel member of the genus phlebovirus in the bunyaviridae family in china. the detailed clinical features of cases with sftsv infection have not been well described, and the risk factors for severity among patients and fatality among severe patients remain to be determined.201324236203
preparation and evaluation of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for detection of total antibodies in human and animal sera by double-antigen sandwich enzyme-linked immunosorbent assay.the recent emergence of the human infection confirmed to be caused by severe fever with thrombocytopenia syndrome virus (sftsv) in china is of global concern. safe diagnostic immunoreagents for determination of human and animal seroprevalence in epidemiological investigations are urgently needed. this paper describes the cloning and expression of the nucleocapsid (n) protein of sftsv. an n-protein-based double-antigen sandwich enzyme-linked immunosorbent assay (elisa) system was set up to detect ...201122135253
epidemiological survey of severe fever with thrombocytopenia syndrome virus in ticks in nagasaki, japan.severe fever with thrombocytopenia syndrome (sfts) is an emerging disease endemic in east asia. transmitted to other organisms by infected ticks, the sfts virus (sftsv) and is endemic to nagasaki in western japan. however, epidemiological information regarding sftsv in nagasaki ticks has not been available to date. in this study, we began by examining the sensitivities of sftsv gene detection by real-time rt-pcr and virus isolation in cultured cells and mice. these methods could detect sftsv in ...201526543390
full-genome sequences of severe fever with thrombocytopenia syndrome virus, isolated from south korea in 2014.here, we present the full-length genome sequencing of severe fever with thrombocytopenia syndrome (sfts) virus, isolated from south korea in 2014. the five korean strains were compared by phylogenetic analysis with full sfts genome sequences of two neighboring nations, china and japan.201525883276
prevalence of sftsv among asian house shrews and rodents, china, january-august 2013.to evaluate the role of small mammals as hosts of severe fever with thrombocytopenia syndrome virus (sftsv), we tested serum samples from rodents and shrews in china, collected in 2013. sftsv antibodies and rna were detected, suggesting that rodents and shrews might be hosts for sftsv.025418111
severe fever with thrombocytopenia syndrome virus, south korea, 2013.during 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in south korea. environmental temperature probably affected the monthly and regional distribution of case-patients within the country. phylogenetic analysis indicated that the isolates from korea were closely related to isolates from china and japan.025341085
characterization of severe fever with thrombocytopenia syndrome in rural regions of zhejiang, china.severe fever with thrombocytopenia syndrome virus (sftsv) infections have recently been found in rural regions of zhejiang. a severe fever with thrombocytopenia syndrome (sfts) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. during the study period of 2011-2014, a total of 51 sftsv infection cases were identified and the case fatality rate was 12% (6/51). ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of la ...201425356556
seroprevalence of severe fever with thrombocytopenia syndrome virus in southeastern china and analysis of risk factors.severe fever with thrombocytopenia syndrome virus (sftsv) has been prevalent for some time in china and it was first identified in 2010. however, the seroprevalence of sftsv in the general population in southeastern china and risk factors associated with the infection are currently unclear. blood samples were collected from seven counties across zhejiang province and tested for the presence of sftsv-specific igg antibodies by elisa. a total of 1380 blood samples were collected of which 5·51% wer ...201424866248
seroprevalence and risk factors for severe fever with thrombocytopenia syndrome virus infection in jiangsu province, china, 2011.severe fever with thrombocytopenia syndrome (sfts), which is caused by a novel bunyavirus, is an emerging infectious disease in china. in 2011, this new virus was designated as severe fever with thrombocytopenia syndrome virus (sftsv). the aim of the present study was to determine the seroprevalence and risk factors of sftsv infection. the investigation was conducted among the general population in jiangsu province, china in 2011. a total of 2,510 serum samples were collected. testing by enzyme- ...201324343883
the pathogenesis of severe fever with thrombocytopenia syndrome virus infection in alpha/beta interferon knockout mice: insights into the pathologic mechanisms of a new viral hemorrhagic fever.severe fever with thrombocytopenia syndrome virus (sftsv) is a newly discovered phlebovirus causing an emerging hemorrhagic fever in east asia, with reported case fatality rates up to 30%. despite the high case fatality rate and large number of persons at risk of infection, the pathobiology of the disease is unknown, and no effective animal model has been available for investigating its pathogenesis. we have studied mice and hamsters as potential small-animal models of sftsv infection following ...201324257618
a reported death case of a novel bunyavirus in shanghai, china.this paper describes the first case of infection with a recently described novel bunyavirus, severe fever with thrombocytopenia syndrome virus (sftsv), in shanghai, china. the case is originally from chizhou city, anhui province within an endemic area for sftsv. we describe the etiology, epidemiological characteristics, clinical diagnosis and treatment of this fatal case. this case is unique because major cause of death was renal failure, whereas other reported cases have been due to hemorrhage. ...201323758684
discovery of severe fever with thrombocytopenia syndrome bunyavirus strains originating from intragenic recombination.this study analyzes available severe fever with thrombocytopenia syndrome virus (sftsv) genomes and reports that a sublineage of lineage i bears a unique m segment recombined from two of three prevailing sftsv lineages. through recombination, the sublineage has acquired nearly complete g1 associated with protective epitopes from lineage iii, suggesting that this recombination has the capacity to induce antigenic shift of the virus. therefore, this study provides some valuable implications for th ...201222933273
severe fever with thrombocytopenia syndrome virus, shandong province, china.severe fever with thrombocytopenia syndrome, which results in severe illness and has a high case-fatality rate, is caused by a novel bunyavirus, severe fever with thrombocytopenia syndrome virus. we found that samples from 2/237 (0.8%) healthy persons and 111/134 (83%) goats in yiyuan county, shandong province, china, were seropositive for this virus.022608264
characterization of the bhanja serogroup viruses (bunyaviridae): a novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses.bhanja virus (bhav) and its antigenically close relatives forecariah virus (forv), kismayo virus (kisv), and palma virus (palv) are thought to be members of the family bunyaviridae, but they have not been assigned to a genus or species. despite their broad geographical distribution and reports that bhav causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemi ...201323325688
development and characterization of monoclonal antibodies directed against the nucleoprotein of heartland virus.heartland virus (hrtv), a phlebovirus first isolated from two missouri farmers in 2009, has been proposed to be transmitted to humans by the bite of infected amblyomma americanum ticks. it is closely related to severe fever with thrombocytopenia syndrome virus (sftsv) from china, another previously unrecognized phlebovirus that has subsequently been associated with hundreds of cases of severe disease in humans. to expand diagnostic capacity to detect hrtv infections, 20 hybridoma clones secretin ...201526503274
malsoor virus, a novel bat phlebovirus, is closely related to severe fever with thrombocytopenia syndrome virus and heartland virus.during a survey in the year 2010, a novel phlebovirus was isolated from the rousettus leschenaultii species of bats in western india. the virus was identified by electron microscopy from infected vero e6 cells. phylogenic analysis of the complete genome showed its close relation to severe fever with thrombocytopenia syndrome (sfts) and heartland viruses, which makes it imperative to further study its natural ecology and potential as a novel emerging zoonotic virus.201424390329
rna encapsidation and packaging in the phleboviruses.the bunyaviridae represents the largest family of segmented rna viruses, which infect a staggering diversity of plants, animals, and insects. within the family bunyaviridae, the phlebovirus genus includes several important human and animal pathogens, including rift valley fever virus (rvfv), severe fever with thrombocytopenia syndrome virus (sftsv), uukuniemi virus (uukv), and the sandfly fever viruses. the phleboviruses have small tripartite rna genomes that encode a repertoire of 5-7 proteins. ...201627428993
genetic characterization of bhanja virus and palma virus, two tick-borne phleboviruses.the genomes of bhanja virus (bhav) and palma virus (palv) two tick-borne viruses hitherto grouped into the bhanja virus antigenic complex of the bunyaviridae were determined by pyrosequencing. phylogenetic analysis groups all three segments of bhav and palv into a distinct clade of tick-borne phleboviruses together with the newly described severe fever with thrombocytopenia syndrome virus and uukuniemi virus. the terminal signature sequences which are signatures for taxonomic grouping and import ...201222806684
a two-tube multiplex real-time rt-pcr assay for the detection of four hemorrhagic fever viruses: severe fever with thrombocytopenia syndrome virus, hantaan virus, seoul virus, and dengue virus.the aim of this study was to develop and evaluate a two-tube multiplex real-time rt-pcr assay for the detection and identification of four viral hemorrhagic fever (vhf) pathogens, severe fever with thrombocytopenia syndrome virus (sftsv), hantaan virus (htnv), seoul virus (seov), and dengue virus (denv), from human clinical samples. the two-tube multiplex real-time rt-pcr assay we developed has a sensitivity of 10 copies/μl for each of the targets, and the performance was linear within the range ...201323532380
entry of severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) is a globe-shaped virus covered by a dense icosahedral array of glycoproteins gn/gc that mediate the attachment of the virus to host cells and the fusion of viral and cellular membranes. several membrane factors are involved in virus entry, including c-type lectins and nonmuscle myosin heavy chain iia. the post-fusion crystal structure of the gc protein suggests that it is a class ii membrane fusion protein, similar to the e/e1 protein of ...201727995422
application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of sftsv-specific human igg and igm antibodies by indirect elisa.severe fever with thrombocytopenia syndrome (sfts) is an emerging disease that was first reported in china in 2011. it is caused by sfts virus (sftsv) which is a member of the phlebovirus genus in the bunyaviridae family. sftsv has been classified as a bsl3 pathogen. there is a need to develop safe and affordable serodiagnostic methods for proper clinical management of infected patients.201526239826
severe fever with thrombocytopenia virus glycoproteins are targeted by neutralizing antibodies and can use dc-sign as a receptor for ph-dependent entry into human and animal cell lines.severe fever with thrombocytopenia syndrome virus (sftsv) is a novel bunyavirus that recently emerged in china. infection with sftsv is associated with case-fatality rates of up to 30%, and neither antivirals nor vaccines are available at present. development of antiviral strategies requires the elucidation of virus-host cell interactions. here, we analyzed host cell entry of sftsv. employing lentiviral and rhabdoviral vectors, we found that the gn/gc glycoproteins (gn/gc) of sftsv mediate entry ...201323388721
structure of severe fever with thrombocytopenia syndrome virus nucleocapsid protein in complex with suramin reveals therapeutic potential.severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel bunyavirus (sftsv). lack of vaccines and inadequate therapeutic treatments have made the spread of the virus a global concern. viral nucleocapsid protein (n) is essential for its transcription and replication. here, we present the crystal structures of n from sftsv and its homologs from buenaventura (bue) and granada (gra) viruses. the structures reveal that phleboviral n folds into a compact core dom ...201323576501
characterization of glycoprotein-mediated entry of severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome (sfts) is an emerging hemorrhagic fever with a high case fatality rate caused by sfts virus (sftsv). effective vaccines and specific therapies for sfts are urgently sought, and investigation into virus-host cell interactions is expected to contribute to the development of antiviral strategies. in this study, we have developed a pseudotype vesicular stomatitis virus (vsv) bearing the unmodified gn/gc glycoproteins (gps) of sftsv (sftsvpv). we have analy ...201626984731
the emergence and cross species transmission of newly discovered tick-borne bunyavirus in china.a novel tick-borne bunyavirus, discovered in china and later in south korea and japan, is now known as huaiyangshan virus or severe fever with thrombocytopenia syndrome virus and has been identified as the causative agent of a hemorrhagic fever-like disease. of five species of ticks carrying huaiyangshan viruses, haemaphysalis longicornis was the most abundant in regions where the virus was endemic. its usual hosts (cattle, goats, dogs, rats and chickens) tested positive for huaiyangshan virus r ...201626949898
development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera.severe fever with thrombocytopenia syndrome (sfts) caused by the sfts virus (sftsv), a phlebovirus in the family bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. this disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in china. because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the sfts circulation in animals and controlling the tick-mammal cycle are ...201626435543
[prevalence and homology analysis on human and animals severe fever with thrombocytopenia syndrome virus infection in yantai of shandong province].to learn the prevalence of infection of human and animals severe fever with thrombocytopenia syndrome bunyavirus (sftsv) in yantai, shandong province, and to analyze the pathogenic features of sftsv as well as its relationship between human and animal hosts.201425059360
a cross-sectional survey of severe fever with thrombocytopenia syndrome virus infection of domestic animals in laizhou city, shandong province, china.a serosurvey of severe fever with thrombocytopenia syndrome virus (sftsv) infection in domestic animals was conducted in the rural areas of laizhou city, shandong province, china to determine strategies for control and prevention of sfts. serum samples were collected from cattle, goats, dogs, pigs, and chickens and antibodies against sftsv were detected by double-antigen sandwich enzyme-linked immunosorbent assay (elisa). of 641 serum samples, the sftsv seropositive rate was 41.8% (268/641): 74. ...201424451093
novel bunyavirus in domestic and captive farmed animals, minnesota, usa.we tested blood samples from domestic and captive farmed animals in minnesota, usa, to determine exposure to severe fever with thrombocytopenia syndrome virus and heartland-like virus. we found antibodies against virus nucleoproteins in 10%-18% of samples from cattle, sheep, goats, deer, and elk in 24 minnesota counties.201323966016
severe fever with thrombocytopenia syndrome virus among domesticated animals, china.to investigate the infections of severe fever with thrombocytopenia syndrome virus (sftsv) in domesticated animals, we sampled a total of 3,039 animals in 2 counties in shandong province, people's republic of china, from april to november 2011. sftsv-specific antibodies were detected in 328 (69.5%) of 472 sheep, 509 (60.5%) of 842 cattle, 136 (37.9%) of 359 dogs, 26 (3.1%) of 839 pigs, and 250 (47.4%) of 527 chickens. sftsv rna was detected in all sampled animal species, but the prevalence was l ...201323648209
first detection of severe fever with thrombocytopenia syndrome virus in the tick species haemaphysalis concinna in shandong province, china.the aim of this study was to detect severe fever with thrombocytopenia syndrome virus (sftsv) infection using polymerase chain reaction (pcr) amplification in adult haemaphysalis concinna ticks. a total of 72 adult h. concinna ticks were obtained from 35 goats, three adult h. concinna ticks (4.17 %) collected from two goats were found to be infected with sftsv via pcr assay. sequence analysis showed that the partial segment m glycoprotein gene of sftsv was about 500 bases long by polymerase chai ...201526350381
the first molecular evidence of severe fever with thrombocytopenia syndrome virus in ticks in jilin, northeastern china.severe fever with thrombocytopenia syndrome (sfts) is an emerging tick-borne zoonosis. the aim of this study was to investigate sfts virus (sftsv) infections in ticks in northeastern china. a total of 6427 ticks, including 5450 haemaphysalis longicornis, 463 dermacentor silvarum, 351 dermacentor nuttalli, and 163 ixodes persulcatus, were sampled in the liaoning, jilin, and heilongjiang provinces of northeastern china. viral megagenomic analysis of the ticks revealed 25 contigs targeting the m an ...201627460903
nano-patterning of a stainless steel microneedle surface to improve the dip-coating efficiency of a dna vaccine and its immune response.dna vaccination with microneedles (mns) into the skin represents a potential therapeutic approach for the clinical treatment of viral diseases as well as for intradermal genetic immunization. in this study, we investigated a dna vaccination against the severe fever with thrombocytopenia syndrome virus (sftsv) delivered by nano-patterned microneedles (nmns) to improve the efficiency compared to a conventional mn vaccination. because dna vaccinations delivered by coated mns have major disadvantage ...201728780461
a cluster of symptomatic and asymptomatic infections of severe fever with thrombocytopenia syndrome caused by person-to-person transmission.severe fever with thrombocytopenia syndrome virus (sftsv) is a novel phlebovirus that was identified to be the etiological pathogen of the emerging infectious disease, severe fever with thrombocytopenia syndrome (sfts). sftsv could be transmitted through tick bite. transmission of sftsv among humans has also been reported mainly through direct blood contact. in july 2014, a cluster of six suspected sfts cases occurred in shandong province, china. in this cluster, both symptomatic and asymptomati ...201728722592
severe fever with thrombocytopenia syndrome virus in humans, domesticated animals, ticks, and mosquitoes, shaanxi province, china.abstractthe first human infection with severe fever with thrombocytopenia syndrome virus (sftsv) was detected in shaanxi province, china, in 2013, although the virus had been reported in 13 other provinces of china since 2010. we collected and analyzed a total of 4,011 samples, including 936 human serum samples, 155 animal serum samples, 895 ticks, 1,950 mosquitoes, 30 midges, and 20 sandflies. sftsv antibodies were found in 44 human samples (4.7%) with no significant differences between males a ...201728719252
differential antagonism of human innate immune responses by tick-borne phlebovirus nonstructural proteins.in recent years, several newly discovered tick-borne viruses causing a wide spectrum of diseases in humans have been ascribed to the phlebovirus genus of the bunyaviridae family. the nonstructural protein (nss) of bunyaviruses is the main virulence factor and interferon (ifn) antagonist. we studied the molecular mechanisms of ifn antagonism employed by the nss proteins of human apathogenic uukuniemi virus (uukv) and those of heartland virus (hrtv) and severe fever with thrombocytopenia syndrome ...201728680969
severe fever with thrombocytopenia syndrome virus infection in minks in china.we analyzed the seroprevalence of tick-borne severe fever with thrombocytopenia syndrome virus (sftsv) in farm-raised minks using double antigen elisa (enzyme-linked immunosorbent assay) kit and indicated that 8.4% (15/178) of the minks had antibodies to the nucleoprotein of sftsv and 72.7% (8/11) of mink farms had minks positive to sftsv. the elisa results were further confirmed by presence of neutralization to sftsv in the mink sera. our results suggested that minks were widely infected with s ...201728654374
molecular identification of novel phlebovirus sequences in european ticks.in recent years the number of newly described tick-borne phleboviruses has been steadily growing. some of these novel viruses are highly pathogenic in humans, e.g. the heartland and severe fever with thrombocytopenia syndrome virus. we aimed to analyse ticks sampled across europe to investigate the diversity of phleboviruses using a comprehensive pcr-based screening approach. a total of 4387 ticks were collected from the vegetation in regions of france, belgium, germany, sweden, and estonia, res ...201728641955
experimental infections of mosquitoes with severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome (sfts) is a newly identified emerging infectious disease, which is caused by a novel bunyavirus (termed sftsv) in asia. although mosquitoes have not been identified as the primary vectors, as revealed by epidemiological surveys, their role in transmitting this sftsv as a suspicious vector has not been validated.201728569189
the amino acid at position 624 in the glycoprotein of sftsv (severe fever with thrombocytopenia virus) plays a critical role in low-ph-dependent cell fusion activity.severe fever with thrombocytopenia syndrome virus (sftsv) is a novel phlebovirus responsible for causing an emerging zoonotic disease. we previously established subclones from sftsv strain yg1 based on differences in low-ph-dependent cell fusion activities and found two amino acid substitutions, y328h and r624w, in the envelope glycoprotein (gp) of high fusion subclones. in this study, we show that transiently expressed gp with the r624w mutation, but not the y328h mutation, induced cell fusion ...201728442665
molecular detection and phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in shelter dogs and cats in the republic of korea.severe fever with thrombocytopenia syndrome is a tick-borne infectious disease. the present study investigated the prevalence of severe fever with thrombocytopenia syndrome virus (sftsv) in shelter dogs and cats in the republic of korea (rok). blood samples were collected from 426 dogs and 215 cats in animal shelters throughout the rok in 2016. of the tested samples, one (0.2%) dog and one (0.5%) cat were positive for sftsv. phylogenetic analysis of the sequences obtained in the present study sh ...201728442241
seroprevalence of severe fever with thrombocytopenia syndrome virus in china: a systematic review and meta-analysis.severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease caused by a novel bunyavirus-sftsv. the seroprevalence of anti-sftsv antibodies including immunoglobulin g (igg) and immunoglobulin m (igm), specific to sftsv in the general population has been investigated in various epidemiological studies with inconsistent results. here, we clarify this discrepancy and reach a more comprehensive result by mean of a meta-analysis.201728399161
a role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry.a novel bunyavirus was recently found to cause severe febrile illness with high mortality in agricultural regions of china, japan, and south korea. this virus, named severe fever with thrombocytopenia syndrome virus (sftsv), represents a new group within the phlebovirus genus of the bunyaviridae. little is known about the viral entry requirements beyond showing dependence on dynamin and endosomal acidification. a haploid forward genetic screen was performed to identify host cell requirements for ...201728388693
seroprevalence of severe fever with thrombocytopenia syndrome virus in hedgehog from china.severe fever with thrombocytopenia syndrome, an emerging hemorrhagic fever, is caused by severe fever with thrombocytopenia syndrome virus (sftsv), a tick-borne bunyavirus. information regarding sftsv animal hosts is very limited. in this study, we showed that 64% (9/14) of hedgehogs in shandong province, china were seropositive to sftsv antibody, suggesting that hedgehog could be a vertebrate parasitifer for sftsv.201728323557
current status of severe fever with thrombocytopenia syndrome in china.severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease caused by sfts virus (sftsv). sftsv is associated with a high mortality rate and has been reported in china, south korea and japan. sftsv undergoes rapid changes owing to evolution, gene mutations, and reassortment between different strains of sftsv. in this review, we summarize the recent cases and general properties of sfts, focusing on the epidemiology, genetic diversity, clinical features, and diagnostics of ...201728251515
severe fever with thrombocytopenia syndrome virus inhibits exogenous type i ifn signaling pathway through its nss invitro.severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease caused by a novel bunyavirus (sfts virus, sftsv). at present there is still no specific antiviral treatment for sftsv; to understand which cells support sftsv life cycle and whether sftsv infection activates host innate immunity, four different cell lines (vero, hela, huh7.5.1, and huh7.0) were infected with sftsv. intracellular/extracellular viral rna and expression of ifnα, and ifnß were detected by real-time ...201728234991
animal models of emerging tick-borne phleboviruses: determining target cells in a lethal model of sftsv infection.the pathogenesis of clinical manifestations caused by newly emerging tick-borne phleboviruses [i.e., severe fever with thrombocytopenia syndrome virus (sftsv) and heartland virus (hrtv)], such as severe thrombocytopenia and lymphocytopenia, are not yet fully understood. in the present study, to establish an animal model mimicking the profile of fatal human cases, we examined the susceptibilities of adult mice from 12 strains, aged mice from two strains, and cynomolgus macaques to sftsv and/or hr ...201728194148
a sensitive and specific rapid diagnostic test for severe fever with thrombocytopenia syndrome virus. 201728188817
identification of a candidate standard strain of severe fever with thrombocytopenia syndrome virus for vaccine quality control in china using a cross-neutralization assay.severe fever with thrombocytopenia syndrome (sfts) is caused by a phlebovirus of the bunyaviridae family, which is designated as sfts virus (sftsv). to our knowledge, no efficient sftsv vaccine exists. here, we report the identification of a standard virus strain for the eight major sftsv strains circulating in china for use in evaluating the sftsv vaccine. rabbits were immunized with the sftsv strains and the cross-neutralization capacities of sftsv anti-sera were determined in microculture cyt ...201728173977
unusual presentation of a severely ill patient having severe fever with thrombocytopenia syndrome: a case report.severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel phlebovirus belonging to the family bunyaviridate. emergence of encephalitis/encephalopathy during severe fever with thrombocytopenia syndrome progression has been identified as a major risk factor associated with a poor prognosis. here we report the case of a severely ill patient with severe fever with thrombocytopenia syndrome virus-associated encephalitis/encephalopathy characterized by a lesion of ...201728153057
antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the virus serodiagnosis. 201728120219
novel sub-lineages, recombinants and reassortants of severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) has been continuously circulating in eastern asia in recent years, without the availability of effective vaccines or antiviral drugs, posing a serious threat to public health, particularly in the central-eastern provinces of china. in this study, we isolated and sequenced four new sftsv strains from shandong province identified in 2015. phylogenetic analysis, with all publicly available l, m and s gene segments, revealed that the four new ...201728117273
a neutralization assay with a severe fever with thrombocytopenia syndrome virus strain that makes plaques in inoculated cells.severe fever with thrombocytopenia syndrome (sfts) is a recently-discovered, potentially fatal infectious disease caused by sfts virus (sftsv). due to the inability of sftsv to make clear cytopathic effects (cpe) in cell culture, titration and neutralization assays of the virus require immunostaining of inoculated cells; consequently, the assays are time-consuming and expensive. in this report, we demonstrate the use of a highly-passaged sftsv strain, p50-2, in a neutralization assay, which made ...201728082164
establishment of subclones of the severe fever with thrombocytopenia syndrome virus yg1 strain selected using low ph-dependent cell fusion activity.the first clinical case of the yg1 strain of the severe fever with thrombocytopenia syndrome virus (sftsv) has been isolated in japan. we found that only some of the cells underwent low ph-dependent cell fusion, although all of the cells were confirmed to have been infected with the virus. this suggested that the yg1 strain consists of a heterogeneous mixture of related viruses. here, we established 3 subclones (termed e3, a4, and b7) from the yg1 strain, using the limiting dilution method with ...201728003599
in vitro antiviral activity of ribavirin against severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease caused by severe fever with thrombocytopenia syndrome virus (sftsv), a novel bunyavirus. as yet, there is no effective antiviral therapy for sfts. ribavirin is a broad-spectrum antiviral agent, which has been tried for treatment of sfts. in this study, antiviral activity of ribavirin against sftsv has been investigated.201727899013
modeling severe fever with thrombocytopenia syndrome virus infection in golden syrian hamsters: importance of stat2 in preventing disease and effective treatment with favipiravir.severe fever with thrombocytopenia syndrome (sfts) is an emerging tick-borne disease endemic in parts of asia. the etiologic agent, sfts virus (sftsv; family bunyaviridae, genus phlebovirus) has caused significant morbidity and mortality in china, south korea, and japan, with key features of disease being intense fever, thrombocytopenia, and leukopenia. case fatality rates are estimated to be in the 30% range, and no antivirals or vaccines are approved for use for treatment and prevention of sft ...201727881648
evidence that processing of the severe fever with thrombocytopenia syndrome virus gn/gc polyprotein is critical for viral infectivity and requires an internal gc signal peptide.the severe fever with thrombocytopenia syndrome virus (sftsv) is an emerging, highly pathogenic bunyavirus against which neither antivirals nor vaccines are available. the sftsv glycoproteins, gn and gc, facilitate viral entry into host cells. gn and gc are generated from a precursor protein, gn/gc, but it is currently unknown how the precursor is converted into the single proteins and whether this process is required for viral infectivity. employing a rhabdoviral pseudotyping system, we demonst ...201627855227
neutralizing antibodies to severe fever with thrombocytopenia syndrome virus 4 years after hospitalization, china.severe fever with thrombocytopenia syndrome is an emerging hemorrhagic fever disease in eastern asia, caused by a tickborne bunyavirus. of 25 patients hospitalized with this disease in china, 100% produced and maintained neutralizing antibodies to severe fever with thrombocytopenia syndrome virus for the study period of 4 years.201627767907
a century in the life of the control of communicable diseases manual: 1917 to 2017.the control of communicable diseases manual, a premier publication of the american public health association, celebrates its centennial in 2017. the control of communicable diseases manual has evolved in format and content through 20 separate editions. this article is a follow-up to an earlier article, titled "evolution of the control of communicable disease manual: 1917 to 2000," that appeared in the journal of public health management & practice in 2001. our update focuses on the period since ...201627682728
presence of antibodies against severe fever with thrombocytopenia syndrome virus in non-endemic areas of china.to determine the seroprevalence of severe fever with thrombocytopenia syndrome (sfts) virus (sftsv) and identify potential risk factors in non-endemic areas, a cross-sectional study was conducted among a healthy population without previously reported sfts in a mountainous area of anhui province. we recruited and collected blood samples from 270 participants in 4 age groups. an enzyme-linked immunosorbent assay kit was used to detect immunoglobulin g (igg) antibodies against sftsv. the overall se ...201727580581
molecular detection of severe fever with thrombocytopenia syndrome virus (sftsv) in feral cats from seoul, korea.this study tested serum samples of feral cats from a highly urbanized habitat, seoul, korea to determine the infection to severe fever with thrombocytopenia syndrome virus (sftsv). from 126 samples tested, sftsv was detected by rt-pcr in 22 (17.5%) cats from various sites of seoul. sequences identified from this study were grouped with clusters from china and japan. our result provides data that sftsv may have been circulating in settings that were suspected to have relatively low risk, such as ...201727542506
[inclusion bodies are formed in sftsv-infected human macrophages].the severe fever with thrombocytopenia syndrome virus (sftsv) is a new member in the genus phlebovirus of the family bunyaviridae identified in china. the sftsv is also the causative pathogen of an emerging infectious disease: severe fever with thrombocytopenia syndrome. using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (np) in sftsv-infected thp-1 cells was investigated with serial doses of sftsv at different times after infection. ...201627295879
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