| a mr 90 000 surface polypeptide of trypanosoma cruzi as a candidate for a chagas' disease diagnostic antigen. | trypanosoma cruzi (peru strain) trypomastigotes and epimastigotes were biosynthetically labeled with [35s]methionine, and the proteins were analyzed by two dimensional polyacrylamide gel electrophoresis (2d-page). 2d-page analysis of the trypomastigotes showed a complex array of polypeptides with distinct clusters at mr 88 000-92 000, isoelectric point (pi) 5.6-6.0, and mr 72 000-76 000, pi 5.6-5.8. 2d-page analysis of the epimastigotes did not show the cluster of polypeptides at mr 90 000. when ... | 1985 | 3932849 |
| leishmanicidal activity of yucatecan medicinal plants on leishmania species responsible for cutaneous leishmaniasis. | the leishmanicidal activity of 15 extracts and 4 pure metabolites obtained from urechites andrieuxii, colubrina greggii, dorstenia contrajerva, and tridax procumbens was evaluated using the newly developed mts ({3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2h-tetrazolium, inner salt) assay, optimized for promastigotes of leishmania major, leishmania tropica, and leishmania aethiopica, as well as for l. aethiopica axenic amastigotes. the assay was then used for calcul ... | 2009 | 18771334 |