| a mr 90 000 surface polypeptide of trypanosoma cruzi as a candidate for a chagas' disease diagnostic antigen. | trypanosoma cruzi (peru strain) trypomastigotes and epimastigotes were biosynthetically labeled with [35s]methionine, and the proteins were analyzed by two dimensional polyacrylamide gel electrophoresis (2d-page). 2d-page analysis of the trypomastigotes showed a complex array of polypeptides with distinct clusters at mr 88 000-92 000, isoelectric point (pi) 5.6-6.0, and mr 72 000-76 000, pi 5.6-5.8. 2d-page analysis of the epimastigotes did not show the cluster of polypeptides at mr 90 000. when ... | 1985 | 3932849 |
| evaluation of antigens from various leishmania species in a western blot for diagnosis of american tegumentary leishmaniasis. | a western blot method that uses antigens from culture promastigote forms of leishmania (viannia) braziliensis, l. (leishmania) amazonensis, l. (leishmania) tropica, and a trypanosomatid (strain 268t) isolated from naturally infected tomatoes was evaluated for laboratory diagnosis of american tegumentary leishmaniasis (atl). serum samples were obtained from 108 patients with atl (group i), 23 chagasic patients (group ii), 32 patients with other diseases (group iii), and 78 healthy individuals (gr ... | 2002 | 12135276 |