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visceral leishmaniasis (kala-azar) as an opportunistic infection in patients infected with the human immunodeficiency virus in spain.in an area endemic for visceral leishmaniasis, 16 patients with human immunodeficiency virus (hiv) infection developed the disease. all belonged to populations at risk for aids (15 were intravenous drug abusers). five patients fulfilled the criteria for full-blown aids, and two more fulfilled them after diagnosis of leishmaniasis. all presented with the classic manifestations of visceral leishmaniasis, but leishmania serology was negative in 15 patients (93%). leishmania donovani amastigotes wer ...19892672242
[imported visceral leishmaniasis (kala azar) in a german infant].we here report the case of a 1 4/12 year old girl with visceral leishmaniasis. returning from a trip to mallorca she presented with pancytopenia, splenomegaly and fever and was admitted to hospital with suspected malignancy. diagnosis was established microscopically from bone marrow smear and confirmed by pcr-assisted amplification of leishmania-specific dna from peripheral blood. treatment was conducted with stibogluconate for 25 days. defervescence and improvement of clinical symptoms was seen ...19958569138
gp63 gene polymorphism and population structure of leishmania donovani complex: influence of the host selection pressure?the gp63 encoding genes were characterized by pcr-rflp in 35 isolates representative of the leishmania donovani complex (l. infantum, l. donovani, l. archibaldi and l. chagasi), with special attention to mediterranean l. infantum from different geographical origins, and in separate groups from old world leishmania (l. major, l. tropica and l. aethiopica). the aim was to evaluate how the possible selective pressure by the host on these important surface proteins would influence structuring of our ...200111197761
absence of leishmania infantum in cave bats in an endemic area in spain.though dogs have been historically considered the main reservoir of leishmania infantum, the role of wildlife in its epidemiology is attracting increasing attention. rodents, wild carnivores and, recently, hares (lepus spp.) have been proposed as sylvatic reservoirs for this parasite. bats have never been tested for l. infantum infection in europe. nevertheless, bats have a widespread distribution, they live in abundant colonies, and some species inhabit caves, where constant temperatures and hu ...201424623348
arbitrary-primed pcr for genomic fingerprinting and identification of differentially regulated genes in indian isolates of leishmania donovani.the arbitrary-primed pcr (ap-pcr) technique was employed with the twin goals of identifying genetic polymorphisms within the indian isolates and to identify differentially expressed gene sequences. the parasite isolates from indian kala-azar patients could be differentiated from leishmania donovani isolates from distinct geographic regions. moreover, differences within the indian isolates could also be identified. a majority (17/19) of the indian isolates gave identical ap-pcr pattern, while two ...200415172218
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