molecular diagnosis of leishmaniosis in dogs. comparative application of traditional diagnostic methods and the proposed assay on clinical samples. | leishmaniosis is a zoonotic, parasitic disease caused by members of the genus leishmania. the disadvantages of the traditional methods have currently rendered the polymerase chain reaction (pcr), the most reliable alternative for the laboratory diagnosis of this disease. several relevant protocols have been described in the past but their application is in most cases limited to research use. the latter combined with the diagnostic problems that can be caused by the genetic variability of the dif ... | 2003 | 12695035 |