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vectors of leishmaniases in venezuela.information on the vectors of leishmaniases in venezuela is reviewed and updated. lutzomyia longipalpis is considered the vector of visceral leishmaniasis. lutzomyia ovallesi of the northcentral area is the only human feeding species from which parasites have been isolated and typed as leishmania braziliensis. in the andean region there is strong evidence that lu. youngi is the vector of cutaneous leishmaniasis but the parasites require further identification. lutzomyia spinicrassa may be respon ...19911841212
zoonotic cutaneous leishmaniasis due to leishmania (viannia) braziliensis associated with domestic animals in venezuela and brazil.after outbreaks of cutaneous leishmaniasis in solano state, venezuela, 5% of the population had parasitized ulcers while after similar outbreaks in mesquita, rio de janeiro state, brazil, 9% had the disease. in these foci children, including some under six years of age, were affected. there was no significant difference in the occurrence of the disease according to sex or type of employment. in solano, 3% of dogs and 28% of donkeys had parasitized lesions, while in mesquita these indices were 19 ...19892319948
natural infection of lutzomyia ovallesi (diptera: psychodidae) with parasites of the leishmania braziliensis complex in a restricted focus of cutaneous leishmaniasis in northern venezuela. 19883271937
first venezuelan record of lutzomyia umbratilis ward & fraiha, 1977 (diptera: psychodidae), a proven vector of leishmania braziliensis guyanensis. 19853832500
a mr 90 000 surface polypeptide of trypanosoma cruzi as a candidate for a chagas' disease diagnostic antigen.trypanosoma cruzi (peru strain) trypomastigotes and epimastigotes were biosynthetically labeled with [35s]methionine, and the proteins were analyzed by two dimensional polyacrylamide gel electrophoresis (2d-page). 2d-page analysis of the trypomastigotes showed a complex array of polypeptides with distinct clusters at mr 88 000-92 000, isoelectric point (pi) 5.6-6.0, and mr 72 000-76 000, pi 5.6-5.8. 2d-page analysis of the epimastigotes did not show the cluster of polypeptides at mr 90 000. when ...19853932849
polymorphism in tumor necrosis factor genes associated with mucocutaneous leishmaniasis.recent studies have shown that mucocutaneous leishmaniasis (mcl), a severe and debilitating form of american cutaneous leishmaniasis (acl) caused by leishmania braziliensis infection, is accompanied by high circulating levels of tumor necrosis factor (tnf)-alpha. analysis of tnf polymorphisms in venezuelan acl patients and endemic unaffected controls demonstrates a high relative risk (rr) of 7.5 (p < 0.001) of mcl disease in homozygotes for allele 2 of a polymorphism in intron 2 of the tnf-beta ...19957595196
diagnosis of cutaneous leishmaniasis and species discrimination of parasites by pcr and hybridization.the aim of this study was to assess the efficacy of pcr methodology in establishing the diagnosis of cutaneous leishmaniasis in patients from areas of endemicity in venezuela. biopsies from 233 patients with cutaneous ulcers suggestive of leishmaniasis were analyzed by pcr, employing oligonucleotides directed against conserved regions of kinetoplast dna (kdna), and the pcr products were then hybridized to nonradioactively labeled, species-specific, cloned kdna fragments. the ability of pcr to de ...19947814554
genomic dna repeat from leishmania (viannia) braziliensis (venezuelan strain) containing simple repeats and microsatellites.in this paper the leishmania (viannia) braziliensis complex is defined as containing all species of the actual subgenus viannia. organisms of the l. (v) braziliensis complex are the causative agents of localized human cutaneous and mucocutaneous leishmaniasis in south america, much of central america and some ares of north america. in our search for better species and subspecies diagnostic probes we focused our research on repetitive dna, since it provides a greater number of target sites for hy ...19979364561
cutaneous leishmaniasis in venezuela caused by infection with a new hybrid between leishmania (viannia) braziliensis and l. (v.) guyanensis. 19979566221
pcr detection of specific leishmania-dna in patients with periodontal disease.this study deals with the detection of leishmania braziliensis dna in gingival specimens from 10 individuals who all had suffered from cutaneous leishmaniasis 5-10 years prior to the examination and all had been treated with anti-leishmaniasis drugs. this preliminary study gives an interesting contribution to the oral microbiology of this disease, with the observation that inflamed periodontal tissues can serve as a factor affecting the dispersion of leishmania parasites in individuals who had s ...200211912876
molecular epidemiology of cutaneous leishmaniasis in venezuela.this paper discusses the utility of a set of primers (3j1, 3j2) designed from a repetitive nuclear deoxyribonucleic acid sequence for the diagnosis of leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in venezuela. a high incidence of leishmania (viannia) braziliensis infection was found in the endemic areas studied. the sensitivity and specificity of the primers used were adequate for the identification o ...200212055822
therapy of venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized leishmania promastigotes and bacillus calmette-guerin: preliminary report.severe mucocutaneous (mcl) and diffuse (dcl) forms of american cutaneous leishmaniasis (acl) are infrequent in venezuela. chemotherapy produces only transitory remission in dcl, and occasional treatment failures are observed in mcl. we have evaluated therapy with an experimental vaccine in patients with severe leishmaniasis. four patients with mcl and 3 with early dcl were treated with monthly intradermal injections of a vaccine containing promastigotes of leishmania (viannia) braziliensis kille ...200415057348
parameters of leishmania braziliensis transmission by indoor lutzomyia ovallesi in venezuela.we developed a mathematical model of cutaneous leishmaniasis (cl) transmission predicting cl incidence based on field data of number of positive sand flies, new cl cases, and number of susceptible people. we estimated the following parameters: a cl incubation period of one month, the overall susceptibility (phi = 0.793), the serologic force of infection (lambda(m) = 0.108 /person/year, sd = 0.014), the clinical force of infection (lambda(l)= 0.114/year), the proportion of infections that result ...200415100449
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