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molecular and morphological characterisation of echinococcus from food producing animals in india.in view of the medical, veterinary and economic importance of hydatid disease in india, our study aimed to determine the prevalence and genotypes of echinococcus present in domestic livestock in india. out of 21,861 animals examined, cattle were found with the highest prevalence of hydatid cysts (5.10%) followed by buffaloes (3.81%), pigs (0.87%) and sheep (0.075%). phylogenetic analysis of the cytochrome oxidase-1 gene revealed that the buffalo strain or g3 genotype was the predominant genotype ...200919632783
a pcr system for detection of species and genotypes of the echinococcus granulosus-complex, with reference to the epidemiological situation in eastern africa.we describe the development of a specific and sensitive pcr/semi-nested pcr system for the rapid diagnosis of echinococcus granulosus genotype g1, e. granulosus genotype g6/7, and echinococcus ortleppi (g5). diagnosis of g1 and the group g5/6/7 is performed by a simple pcr, while discrimination between e. ortleppi (g5) and g6/7 involves a subsequent semi-nested pcr step. the target sequence for amplification is part of the mitochondrial 12s rrna gene. specificity of the pcrs was 100% when evalua ...200415064129
the present situation of echinococcosis in europe.the taxonomy of echinococcus is presently undergoing major changes, the paraphyletic echinococcus granulosus being split into several distinct species. in this review, an attempt is made to assess the present epidemiological situation in europe separately for each species (echinococcus multilocularis, echinococcus granulosus sensu stricto, echinococcus equinus, echinococcus ortleppi, and echinococcus sp.). for e. multilocularis, an increasing density of infected host animals is apparent in centr ...200616352465
echinococcus granulosus cattle strain identification in an autochthonous case of cystic echinococcosis in central mexico.echinococcosis is a frequent hepatic parasitic disease in several countries but it is practically absent in mexico. a cattle strain of echinococcus granulosus was identified by rapd, pcr-rflp and mitochondrial co1 gene analysis in an autochthonous case. the parasite was obtained after a laparoscopic excision of a liver cyst from a patient that was symptomatic for 6 years but mis-diagnosed before hospitalization.200415533292
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