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bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste.a strictly anaerobic bacterial strain, designated xdt-1t, was isolated from plant residue from a methanogenic reactor treating waste from cattle farms. cells of the strain were gram-negative, non-motile, non-spore-forming rods. haemin was required for growth. the strain utilized xylan as well as various sugars including arabinose, xylose, glucose, mannose, cellobiose, raffinose, starch and pectin. the strain produced acetate, propionate and succinate from saccharides in the presence of haemin. t ...200919567576
distribution and mobility of the tetracycline resistance determinant tetq.we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ...19979372425
evaluation of new gyrb-based real-time pcr system for the detection of b. fragilis as an indicator of human-specific fecal contamination.a rapid and specific gyrb-based real-time pcr system has been developed for detecting bacteroides fragilis as a human-specific marker of fecal contamination. its specificity and sensitivity was evaluated by comparison with other 16s rrna gene-based primers using closely related bacteroides and prevotella. many studies have used 16s rrna gene-based method targeting bacteroides because this genus is relatively abundant in human feces and is useful for microbial source tracking. however, 16s rrna g ...201020643165
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