| tickborne arbovirus surveillance in market livestock, nairobi, kenya. | to identify tickborne viruses circulating in kenya and the surrounding region, we conducted surveillance at abattoirs in nairobi, kenya. species of ticks collected included rhipicephalus pulchellus (56%), amblyomma gemma (14%), r. appendiculatus (8%), a. variegatum (6%), and others. a total of 56 virus isolates were obtained, 26 from a. gemma, 17 from r. pulchellus, 6 from a. variegatum, and 7 from other species. virus isolates included dugbe virus (dugv), an unknown virus related to dugv, thogo ... | 2006 | 16836823 |
| host and viral traits predict zoonotic spillover from mammals. | the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ... | 2017 | 28636590 |
| investigation of tick-borne viruses as pathogens of humans in south africa and evidence of dugbe virus infection in a patient with prolonged thrombocytopenia. | in the course of investigating suspected cases of viral haemorrhagic fever in south africa patients were encountered who had been bitten by ticks, but who lacked evidence of infection with crimean-congo haemorrhagic fever (cchf) virus or non-viral tick-borne agents. cattle sera were tested by enzyme-linked immunoassay to determine whether tick-borne viruses other than cchf occur in the country. the prevalence of antibody in cattle sera was 905/2116 (42.8%) for cchf virus, 70/1358 (5.2%) for dugb ... | 1996 | 8666081 |
| development of a one step real time rt-pcr assay to detect and quantify dugbe virus. | a one-step real time quantitative rt-pcr (qrt-pcr) assay was developed to detect all published dugbe virus (dugv) genomes of the nairovirus genus. primers and probes were designed to detect specific sequences on the most conserved regions of the s segment. the limit of detection of the assay was 10 copies per reaction which is an improvement of 3 log(10)ffu/ml over the sensitivity of conventional rt-pcr. the specificity of the primers and probe was confirmed with the closely related nairoviruses ... | 2011 | 21703306 |
| kupe virus, a new virus in the family bunyaviridae, genus nairovirus, kenya. | we have previously described isolation and preliminary identification of a virus related to dugbe virus (dugv), family bunyaviridae, genus nairovirus. six isolates of the virus were obtained from pools of amblyomma gemma and rhipicephalus pulchellus ticks collected from hides of cattle in nairobi, kenya, in october 1999. we report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show ... | 2009 | 19193256 |