| prevalences of schistosoma japonicum infection in reservoir hosts in south-western china. | | 2010 | 20406586 |
| parasite genetic differentiation by habitat type and host species: molecular epidemiology of schistosoma japonicum in hilly and marshland areas of anhui province, china. | schistosoma japonicum, a parasite of significant public health importance in parts of china and southeast asia, is a true generalist pathogen with over 40 species of mammals suspected as definitive host reservoirs. in order to characterize levels of parasite gene flow across host species and identify the most important zoonotic reservoirs, s. japonicum larvae (miracidia) were sampled from a range of definitive host species in two contrasting habitat types within anhui province, china: a low-lyin ... | 2009 | 19389178 |
| does multiple hosts mean multiple parasites? population genetic structure of schistosoma japonicum between definitive host species. | multi-host parasites, those capable of infecting more than one species of host, are responsible for the majority of all zoonotic, emerging or persistent human and animal diseases and are considered one of the major challenges for the biomedical sciences in the 21st century. we characterized the population structure of the multi-host parasite schistosoma japonicum in relation to its definitive host species by genotyping miracidia collected from humans and domestic animals across five villages aro ... | 2006 | 16876170 |
| schistosoma japonicum eggs survive passage through dogs and chickens. | the present study sought to elucidate whether schistosoma japonicum eggs could survive a passage through dogs and chickens. three dogs and three chickens were used for the experiment. the dogs were allowed to eat goat faeces containing 80 s. japonicum eggs per gram. the chickens were given 2 ml of water containing more than 100 s. japonicum eggs per ml. the hatchability of the eggs was 51.8% prior to administration. all faeces were collected for 3 days from the dogs and for 2 days from the chick ... | 2006 | 16650599 |
| population genetics of multi-host parasites--the case for molecular epidemiological studies of schistosoma japonicum using larval stages from naturally infected hosts. | population genetics of multi-host pathogens offers great potential for the understanding of their complex epidemiology but care must be taken to ensure that the sampling procedure does not bias estimates of population indices. the transfer of material to laboratory passage, in particular, runs the risk of bottlenecking and imposing non-random host-induced selection pressures according to the hosts used in passage. we present a novel technique allowing single-locus microsatellite genotyping of th ... | 2005 | 16255820 |
| transmission of schistosoma japonicum by humans and domestic animals in the yangtze river valley, anhui province, china. | the aim of the present work was to assess the relative contribution to transmission of schistosoma japonicum by humans and domestic animals in two villages in the yangtze river valley in anhui province, china. a cross-sectional survey was conducted to determine the prevalence and intensity of s. japonicum in humans, cattle, water buffaloes, horses, pigs, goats, dogs and cats. additionally, for each host species the number of individuals and the mean faecal excretion per day was determined. resul ... | 2005 | 16188215 |
| silver-enhanced colloidal gold metalloimmunoassay for schistosoma japonicum antibody detection. | a silver-enhanced colloidal gold metalloimmunoassay has been proposed for the determination of schistosoma japonicum antibody (sjab) in rabbit serum. the adult worm antigen of s. japonicum (sjag) was adsorbed passively on the walls of a polystyrene microwell and then reacted with the desired sjab. the colloidal gold-labeled goat anti-rabbit igg secondary antibody was adsorbed on the walls of the polystyrene microwells through the reaction with sjab, followed by the silver enhancement process, di ... | 2005 | 16018885 |
| field evaluation of a test for praziquantel resistance in schistosoma sp. | a simple rapid test for detecting praziquantel resistance in schistosoma sp., involving change in shape of miracidia on exposure to praziquantel, was evaluated in china. tests on miracidia hatched from eggs collected from naturally infected goats were run in a field laboratory in jiangxi province and a research laboratory in shanghai. the mean values in the two laboratories were not significantly different, but the variation between individual samples in the two laboratories suggests that a deli ... | 2003 | 12651219 |
| [protective immunity induced by anti-idiotypic monoclonal antibody np30 of schistosoma japonicum in goats]. | to observe the protective immunity in goats induced by active immunization with anti-idiotypic monoclonal antibody np30 of s. japonicum. | 2000 | 11798794 |
| [antibody detection (ifat, elisa) in experimental schistosoma japonicum-infected goats]. | | 1984 | 6370227 |
| characteristics of the anti-sheep red blood cell heterophile antibodies produced during schistosoma japonicum infection in mice. | heterophile antibodies (ab) against sheep red blood cells were shown to have developed in mice experimentally infected with schistosoma japonicum (sj). incidence of the elevated ab levels was markedly high in the period of 6-10 weeks following infection. by means of absorption tests, the heterophile ab could be distinguished from classical human heterophile ab of paul-bunnell, hanganutzui-deicher, and forssman types. antigen(s) cross-reactive with the heterophile ab was found only in goat red bl ... | 1985 | 3917982 |
| vaccination of goats with 31 kda and 32 kda schistosoma japonicum antigens by dna priming and protein boosting. | two schistosoma japonicum vaccine candidate antigens sj 31 and sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a dna priming-protein boosting strategy in present work. dna vaccine formulations of the two antigens (vrsj31 and vrsj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rsj31 and rsj32) together with freund complete adjuvant (fca) were used to boost the goats. the ... | 2007 | 17571462 |
| the presence of anti-sheep red blood cell heterophile antibodies and their characteristics in murine schistosomiasis japonica. | sensitive methods of an enzyme-linked immunosorbent assay (elisa) using red blood cells (rbc) have been developed and were applied to the detection of anti-sheep red blood cell (srbc) heterophile antibodies (ab) present in sera of schistosoma japonicum (sj)-infected mice. the indirect hemagglutination test (iha) was used for the purpose as well. by these methods a significant increase in the heterophile ab levels was demonstrated in the mice particularly after 6-10 weeks of infection. the hetero ... | 1984 | 6394971 |
| an outbreak of schistosomiasis japonica in taiwan. | the present study was undertaken to determine the current status of schistosoma japonicum infection in the newly found endemic focus at hanpao farm, fangyuan district, changhua county, west-central taiwan. two field surveys and one laboratory investigation were conducted from july to october 1987. of 705 snails examined by shedding and crushing methods, 17.0% were found to harbor cercariae of s. japonicum. by sodium sulfate-acid-ether-triton centrifugal sedimentation technique, 25% of 16 cattle, ... | 1988 | 3151482 |
| a reusable and specific protein a-coated piezoelectric biosensor for flow injection immunoassay. | a hydrophilic matrix of periodate-oxidized dextran was used as a double-sided linker to covalently immobilize staphylococcus aureus protein a (spa) molecules onto a poly-l-lysine-modified piezoelectric crystal surface to improve their stability, activity, and binding specificity with human immunoglobulin g (igg) in flow injection assays. the prepared sensing crystals displayed best sensitivity and reusability at a flow rate of 140 microl/min. a human igg concentration as low as 0.3 nm can be det ... | 2000 | 10662499 |