| fibroblast stimulation in schistosomiasis. i. stimulation in vitro of fibroblasts by soluble products of egg granulomas. | to gain further understanding of the pathogenesis of hepatic fibrosis in schistosomiasis, the interaction between egg granulomas and fibroblasts was investigated in an in vitro model. egg granulomas isolated from livers of mice infected with schistosoma mansoni or schistosoma japonicum and cultured in vitro released a nondialyzable substance which stimulated proliferation in resting dermal fibroblasts. the release of the fibroblast-stimulating substance remained relatively constant during the fi ... | 1981 | 6268716 |
| an epidemiological study on schistosoma japonicum in domestic animals in leyte, philippines. | an epidemiological study of schistosoma japonicum in domestic animals in two municipalities of the eastern coastal plain of leyte, philippines, showed that pigs and dogs have the highest rates of prevalence. dogs had the highest mean 24-hour egg output, and pigs the proportion of hatchable eggs. however, dogs serve an important role in maintaining the transmission of the parasite as indicated by a high transmission potential and the close habitual contact of the animals to human. although necrop ... | 1982 | 7170639 |
| anti-fecundity immunity induced in pigs vaccinated with recombinant schistosoma japonicum 26kda glutathione-s-transferase. | we have recently reported (liu et al. 1995) that immunization of mice with recombinant 26kda gst (resjc26gst) induces a pronounced anti-fecundity effect after experimental infection with chinese schistosoma japonicum. a similar vaccination trial was thus carried out on pigs, important reservoirs for schistosomiasis japonica, using purified, resjc26gst and resjp26gst from schistosoma japonicum with alum as adjuvant; in general, similar results were obtained with the two sources of recombinant 26k ... | 1995 | 8552408 |
| characterization of the gene encoding mhp1 from mycoplasma hyopneumoniae and examination of mhp1's vaccine potential. | the gene encoding mhp1, a 124 kda protein from mycoplasma hyopneumoniae, has been cloned, sequenced, and its product characterized. no significant homology to the gene or encoded polypeptide was found in the genbank, nbrf, or pir databases, though this protein appears similar to p97, a putative adhesin of m. hyopneumoniae described by zhang et al. (infect. immun. 63, 1013-1019, 1995). two repeated motifs were identified within the 3' end of the gene and encoded polypeptide. the mhp1 gene was fus ... | 1997 | 9041663 |
| schistosoma japonicum in the pig: the host-parasite relationship as influenced by the intensity and duration of experimental infection. | parasitologic, clinicopathologic, and pathologic aspects of schistosoma japonicum infections of varying durations and intensities were studied in growing pigs injected intramuscularly with a dose of either 0, 100, 500, or 2,000 cercariae and killed at 4, 11, 17, or 24 weeks postinfection (pi). the number of viable worm pairs decreased significantly in the high dose group after 11 weeks pi but not in the lower dose groups; however, a stable population of immature worms persisted throughout the st ... | 1998 | 9502611 |
| congenital transmission of schistosoma japonicum in pigs. | congenital transmission of schistosoma japonicum in pigs was investigated by experimentally infecting sows at four weeks gestation (n = 3), 10 weeks gestation (n = 3), or a few weeks prior to insemination (n = 2). none of the piglets born to sows infected prior to insemination or in early pregnancy were found to be infected. however, all of the piglets (n = 26) born to sows infected at 10 weeks gestation were found to harbor schistosomes with s. japonicum eggs recovered from both their feces and ... | 1999 | 10072157 |
| the epidemiology and host-parasite relationships of schistosoma japonicum in definitive hosts. | | 1999 | 10366824 |
| peroral infection of pigs with schistosoma japonicum cercariae. | infections with the zoonotic trematode, schistosoma japonicum in pigs serves as a valuable model for studying natural definitive host/parasite relationships and a model for human schistosomosis japonica. in the present study the efficiency of a peroral infection route was compared with that of an intramuscular route of infection. eleven specific pathogen-free danish landrace/yorkshire/duroc crossbred male and female pigs were divided into two groups of five and six pigs, respectively. each pig w ... | 1999 | 10392971 |
| vaccination of domestic pig with recombinant paramyosin. against schistosoma japonicum in china. | paramyosin (pm), a myosin-like protein is a major antigen on schistosoma japonicum (sj). we reported that passive transfer of a monoclonal ige sje18varepsilon.1 which recognizes pm of sj (sjpm), partially protected mice from challenge infection. in the present study, we developed an experimental model system of schistosomiasis japonica with domestic pigs in china and used it for the evaluation of vaccination with recombinant sjpm (rsjpm). sixteen-week-old pigs were successfully infected by derma ... | 2000 | 10715529 |
| tissue responses in experimental schistosomiasis japonica in the pig: a histopathologic study of different stages of single low- or high-dose infections. | the tissue responses of pigs exposed to either 100 or 2,000 schistosoma japonicum cercariae were examined at 4, 11, 17, and 24 weeks postinfection (pi) to explore the pig as an animal model for pathologic aspects of human schistosomiasis japonica. egg granulomas were present in the liver, intestine, and occasionally in the lungs from 11 weeks pi. there were also many free eggs and early exudative reactions to eggs in the intestine. at 11 weeks pi, pigs in the higher dose group showed marked peri ... | 2000 | 10761723 |
| recovery and distribution of ascaris suum superimposed on a schistosoma japonicum infection in pigs. | the aim of this study was to examine the effect of a primary patent schistosoma japonicum infection on the establishment and location of a superimposed ascaris suum infection in pigs. the study comprised two experiments each containing two groups of pigs. in the first experiment, 7 pigs were injected intramuscular (i.m.) with 800 s. japonicum cercariae and inoculated with 1,000 a. suum eggs 11 weeks post primary infection (ppi) and 8 pigs were inoculated with 1,000 a. suum eggs at the time of ch ... | 1998 | 10772553 |
| schistosoma japonicum: day to day variation in excretion and hatchability of parasite eggs in the domestic pig, suis suis. | the aim of the present study was to describe the course of an schistosoma japonicum infection in individual pigs over time, with special regards to fecal egg counts and egg hatchability, emphasizing the extent of variation during and between days. five specific pathogen free danish landrace/yorkshire/duroc crossbred male pigs were each given 3500 cercariae intramuscularly. from day 36 to 62 post infection, fecal samples were collected from each pig, morning and evening. pigs were perfused 62 day ... | 2000 | 10864513 |
| schistosoma japonicum infection in the pig as a model for human schistosomiasis japonica. | valuable information on human schistosomiasis japonica has been provided using primates and experimental rodent hosts. however, major drawbacks such as high costs and ethical concerns for the primate models and large biological deviations for the rodent models have led to the search for more appropriate models. recent data on the pig indicate that this natural host for schistosoma japonicum might be a realistic alternative. as only very few research groups have investigated the s. japonicum/pig ... | 2000 | 10936567 |
| prevalence and distribution of pig helminths in the dongting lake region (hunan province) of the people's republic of china. | the prevalence of helminths in pigs was investigated in five rural communities situated on the embankment of dongting lake in zhiyang county, hunan province, people's republic of china, in an area known to be endemic for schistosoma japonicum. the helminth prevalences identified on the basis of faecal egg count analysis were: oesophagostomum spp. (86.7%), ascaris suum (36.7%), metastrongylus spp. (25.8%), strongyloides spp. (25.8%), trichuris suis (15.8%), globocephalus spp. (6.7%), gnathostoma ... | 2000 | 10831052 |
| postnatal challenge infections of congenitally schistosoma japonicum-infected piglets. | the aim of the present study was to assess the effect of a congenital schistosoma japonicum infection on the establishment, fecundity, and pathogenicity of a postnatal challenge infection. five prenatally s. japonicum-infected piglets received a challenge infection (prenatal + challenge group), 5 prenatally infected piglets were followed without challenge (prenatal group), and 10 piglets, born by unexposed sows, served as challenge controls (challenge control group). challenge infections were gi ... | 2001 | 11534646 |
| schistosoma japonicum in the pig: the influence of age on the host/parasite relationship. | the current study sought to elucidate a possible association between age and susceptibility to a primary infection with schistosoma japonicum in pigs. sixteen landrace/yorkshire crossbred specific pathogen-free pigs in three different age groups (group a-c), aged approximately 7, 24 and 37 weeks at the beginning of the experiment, were infected by intramuscular injections of 1,000, 1,500 or 2,400 cercariae, respectively. fecal egg counts were obtained twice weekly from six to eight weeks post in ... | 1999 | 10695784 |
| experimental schistosomiasis japonica in the pig: immunohistology of the hepatic egg granuloma. | use of the pig as an animal model in schistosomiasis research is increasing, but knowledge of the porcine immune response to schistosome infection is still very limited. we investigated the immunohistology of different maturation stages of the schistosoma japonicum egg granuloma in pigs. liver sections from pigs experimentally infected with s.japonicum for 9, 12 or 21 weeks were examined by immunohistochemistry using a three-step streptavidin-biotin-complex/immunoperoxidase method or a two-step ... | 2002 | 11982860 |
| schistosoma japonicum infection and serum and tissue concentrations of retinol and zinc in pigs. | the effects of schistosoma japonicum infection on the concentrations of zinc in serum, liver, spleen and muscle and on the concentrations of retinol in serum and liver were studied in 48 pigs. twenty-four of the pigs were each infected by intramuscular inoculation with 2000 cercariae of s. japonicum in medium and the rest were similarly inoculated with parasite-free medium, as controls. on each of weeks 4, 11, 17 and 24 post-inoculation (pi), 12 pigs (six of which were infected) were killed. tis ... | 1999 | 10690244 |
| [preliminary study of cercaria antigen of schistosoma japonicum before and after ultraviolet irradiation]. | to study the changes in the constituents of the cercaria antigen of schistosoma japonicum before and after ultraviolet irradiation. | 2002 | 12376252 |
| indirect elisas based on recombinant and affinity-purified glycoprotein e of aujeszky's disease virus to differentiate between vaccinated and infected animals. | two indirect elisas for the detection of antibodies against glycoprotein e (ge) of aujeszky's disease virus (adv) in sera have been developed. the rec-ge-elisa is based on the e. coli-expressed recombinant protein containing the n-terminal sequences of ge (aa 1-125) fused with the glutathione s-transferase from schistosoma japonicum. the affi-ge-elisa is based on native ge, which was purified from virions by affinity chromatography. the tests were optimised and compared with each other, as well ... | 1999 | 10213939 |
| [obtaining and construction of a novel gene from schistosoma japonicum by expressed sequence tags]. | to look for new candidate vaccine molecule and characterize new genes from schistosoma japonicum. | 2002 | 12567540 |
| [investigation on schistosoma japonicum infection in rodents in yunnan province]. | to investigate the role of rodents in the transmission of schistosomiasis in endemic areas in yunnan province. | 2000 | 12567670 |
| a new technic for counting schistosoma japonicum eggs in pig feces. | an improved laboratory method was developed for counting schistosoma japonicum eggs in pig feces, which involves filtration, sedimentation and centrifugation, but avoids toxic chemicals. it is sensitive, allows easy differentiation from similar-sized and shaped protozoan cysts, and permits evaluation of egg viability both by direct viewing of eggs and miracidial hatching. it was found to be significantly better at recovering eggs than the modified bell filtration technic. the sensitivity, specif ... | 1998 | 9740285 |
| effect of praziquantel treatment on experimental porcine schistosoma japonicum infection. | the aims of the study were to assess the effect of praziquantel on schistosoma japonicum infection in pigs, and to elucidate the level of resistance to reinfection after treatment. pigs were given a single infection with s. japonicum followed by a praziquantel treatment (40 mg/kg) week 8, reinfection week 12 and perfusion week 20 post-primary infection. relevant control groups were included. worm burdens, faecal and tissue egg counts, gross- and histopathology of the liver and specific liver enz ... | 1998 | 9651934 |
| in vitro maintenance of schistosoma japonicum and surgical transfer from donor to naïve recipient pigs. | an objective of this study was to find a culture medium and a temperature range suitable for in vitro maintenance of adult schistosoma japonicum during surgical transplantation experiments. adult s. japonicum were cultivated in four different media (nctc 135, nctc 109, rpmi 1640 and 0.85% physiological saline) supplemented with 10% heat-inactivated normal pig serum (hinps) at either 4 degrees c, 22-25 degrees c (room temperature) or 37 degrees c. based on survival and morphologic evaluation, nct ... | 1997 | 9477499 |
| schistosoma japonicum and trichuris suis infections in pigs fed diets with high and low protein. | the aim of the study was to measure the impact of schistosoma japonicum and trichuris suis infections in young growing pigs fed low- or high-protein diets. thirty-two pigs. 6-10 weeks old, were randomly allocated to 2 groups receiving either a high- or a low-protein diet. after 11 weeks half of the pigs from each group were infected with 1500 s. japonicum cercariae and 4000 t. suis eggs. the weight of the pigs was measured throughout the study, and blood and faecal samples were collected every s ... | 1997 | 9300463 |
| recovery of schistosoma japonicum from experimentally infected pigs by perfusion of liver and mesenteric veins. | an optimized procedure for perfusion of pigs infected with schistosoma japonicum was developed. the technique involves insertion of a perfusion influx tube into the thoracic descending aorta, clamping vessels to parts of the body which did not need to be perfused (the kidneys, hind legs, etc.) and placing a collection tube directly into the portal vein. in addition, the clamping technique allows for separate perfusion of the liver and intestinal veins. the perfusion medium was a sodium citrate b ... | 1997 | 9257450 |
| the pig as a unique host model for schistosoma japonicum infection. | | 1996 | 15275220 |
| persistence of the protective immunity to schistosoma japonicum in chinese yellow cattle induced by recombinant 26kda glutathione-s-transferase (resjc26gst). | to observe the long lasting effect of the recombinant sj26gst sub-unit vaccine against schistosoma japonicum in cattle, animals aged from 5 to 12 months were vaccinated with resjc26gst, and were challenged by natural infection 6 months or 12 months after vaccination. worm burdens per cattle and egg burden in tissue (per gram) of cattle with or without vaccination were compared. the results showed that anti-resjc26gst antibodies were produced in vaccinated cattle. following natural infection, the ... | 2004 | 15325043 |
| anti-fecundity immunity to schistosoma japonicum induced in chinese water buffaloes (bos buffelus) after vaccination with recombinant 26 kda glutathione-s-transferase (resjc26gst). | we have shown previously that immunisation of mice and pigs with recombinant 26 kda gst (resjc26gst) induces a pronounced anti-fecundity effect after experimental infection with chinese schistosoma japonicum. we report here that anti-fecundity immunity can also be induced against resjc26gst in chinese water buffaloes (bos buffelus), important reservoir hosts for s. japonicum in china. anti-sjc26gst antibodies were produced in immunised buffaloes and, following challenge with s. japonicum cercari ... | 1997 | 9187028 |
| the effect of a combined approach to schistosomiasis control on the transmission of schistosoma japonicum in xingzi of poyang lake area, china. | the impact of a combined approach to schistosomiasis control from 1987 to 1989 and mass chemotherapy from 1992 to 1994 was studied in a rural community in xingzi county in the northwest corner of poyang lake in jiangxi province, china. humans, cattle, buffalo and pigs were known potential reservoirs of schistosoma japonicum. transmission occurs during water contact on and around seasonally flooded marshes that are used for grazing, the harvesting of grass and fishing. humans and livestock underw ... | 1996 | 9185265 |
| schistosoma japonicum infection in the pig: the effect of a patent primary infection on a challenge infection. | the response of pigs to a challenge infection of schistosoma japonicum following a primary infection was assessed using parasitological parameters and eosinophil counts. twenty-five danish landrace/yorkshire/duroc crossbred pigs were divided into four groups. group a (n = 10) received a primary infection, group b (n = 5) received both a primary and challenge infection, group c (n = 5) received a challenge control infection and group d (n = 5) received no infection serving as helminth-free contro ... | 1997 | 9177096 |
| eosinophil chemotactic factor release from neutrophils induced by stimulation with schistosoma japonicum eggs. | the neutrophil is one of the sources of eosinophil chemotactic factor (ecf) in the presence of some stimulants. in the present study we showed that guinea-pig neutrophils could release ecf upon stimulation with schistosoma japonicum eggs. ecf release from neutrophils began as early as 5 min after the stimulation and reached a peak at 20 min. when homogenate of the eggs was separated into a water-soluble fraction as soluble egg antigen (sea) and a water-insoluble fraction (eggshell), both prepara ... | 1997 | 9000232 |
| a methodological study on egg counts in tissues from pigs infected with schistosoma japonicum. | the present study examined the possibility of using a tissue subsample to estimate the total number of schistosoma japonicum eggs in the liver or an intestinal section. sixteen male landrace/yorkshire crossbred pigs were each infected with 500 s. japonicum cercariae and killed 12 weeks post infection. five-gram samples were taken from specific areas of the liver, colon, caecum and rectum, and tissue egg counts (tecs) were determined by digestion of the tissue sample in 3% koh for 18 h according ... | 1996 | 8916397 |
| worm establishment and egg production of schistosoma japonicum in pigs infected by percutaneous methods or intramuscular injection. | worm establishment and egg production were compared in landrace/yorkshire crossbred pigs infected with schistosoma japonicum using 3 different infection techniques. s. japonicum worm establishment and overall egg production following intramuscular injection of cercariae suspended in iscove's medium exceeded that in pigs infected by the leg immersion or the coverslip percutaneous technique. however, the egg production per mature female was similar for all three infection methods. the results supp ... | 1996 | 8750693 |
| immunization of pigs against infection with schistosoma japonicum using ultraviolet-attenuated cercariae. | since pigs are important in the zoonotic transmission of schistosomiasis japonica in china, a veterinary vaccine might contribute to the control of the disease in humans. pigs were immunized with three doses each of 10,000 cercariae of schistosoma japonicum attenuated with ultraviolet light (400 microwatt.min/cm2). the experiment was performed with portable irradiation equipment in a rural area of the hubei province (p.r. china). a challenge infection of 1,000 untreated cercariae was given 2.5 o ... | 1993 | 8341581 |
| experimental infection of danish landrace/yorkshire crossbred pigs with schistosoma japonicum from the people's republic of china. | this study was undertaken to assess the suitability of danish landrace/yorkshire (l/y) crossbred pigs as experimental hosts of a chinese mainland strain of schistosoma japonicum. pigs were exposed to 200, 500 or 1000 cercariae and parasite burdens were determined by perfusion after either 8 or 11 weeks. all pigs became infected with onset of faecal egg excretion 6 to 7 weeks following exposure to cercariae. the pattern of faecal egg excretion differed markedly among the individual animals. gross ... | 1994 | 7676923 |
| clinical and pathologic features of experimental schistosoma japonicum infection in pigs. | nine piglets, 2-5 months old and weighing 12-15 kg were infected percutaneously with 5000 to 6000 cercariae of schistosoma japonicum. three similar piglets which were not infected served as controls. infected animals were necropsied at 5, 10, 30, 40, 59, 90, 120, 150 and 180 days post-infection (pi) and the controls at 5, 60 and 180 days pi. the prepatent period varied from 27 to 33 days after infection. clinical signs observed, coincident with egg production, were loss of appetite, lethargy, pa ... | 1984 | 6543061 |
| schistosoma japonicum: some parameters affecting the development of protective immunity induced by a cryopreserved, irradiated schistosomula vaccine in guinea-pigs. | experiments were conducted in guinea-pigs to elucidate the parameters affecting the development of protective immunity against schistosoma japonicum induced by a cryopreserved, irradiated schistosomula vaccine such as the number of immunizations, route of injection and the use of adjuvants. results obtained indicated that the cryopreserved, irradiated schistosomula vaccine was effective by either intradermal or intramuscular injection. one intradermal injection with bcg adjuvant resulted in an a ... | 1991 | 1903879 |
| comparison of the vaccine efficacy of gamma-irradiated schistosoma japonicum cercariae with the defined antigen sj62(irv-5) in pigs. | development of a vaccine against schistosoma japonicum which can protect both man and the domestic animal zoonotic reservoirs of infection would be an invaluable tool in attempts to control this infection in those areas in which conventional control methods have failed to break transmission. the pig is a natural host of s. japonicum and because of its anatomical and immunological similarities to humans, it is a potentially valuable host for studies on s. japonicum in particular and schistosomes ... | 2001 | 11522406 |
| cytokine mrna expression in pigs infected with schistosoma japonicum. | the pig is a natural host of schistosoma japonicum and a useful animal model of human disease. in the present study mrna levels of th1 (ifn-gamma) and th2 (il-10 and il-4) cytokines were assessed by rt-pcr within tissues from infected pigs. twelve danish crossbred pigs were infected by intramuscular injection or orally with 1000 cercariae. six other pigs served as non-infected controls. liver and intestinal tissues were collected 10 weeks post-infection, and analysed for their relative levels of ... | 2001 | 11289066 |
| pathology and course of natural schistosoma japonicum infection in pigs: results of a field study in hubei province, china. | in order to obtain information on the natural course of porcine infection with schistosoma japonicum, pigs were exposed to the cercariae of this parasite in a highly endemic region of china. five, 5-month-old pigs previously infected with s. japonicum (group a) and 10, schistosome-naïve piglets (group b) were allowed on a pasture infested with oncomelania snails for one transmission period (approximately 5.5 months). all the piglets rapidly acquired infection, and both groups remained infected t ... | 2000 | 10983559 |
| immunomodulation of the hepatic egg granuloma in schistosoma japonicum-infected pigs. | immunomodulation of perioval granulomas is a well-known phenomenon in schistosome-infected mice, but only little is known about granuloma modulation in other animal models of human schistosomiasis. in the present study, we explored immunomodulation of egg granulomas in the liver in a pig model of schistosomiasis japonica. granuloma size was measured and t cells, b cells and igg(+) plasma cells in granulomas were quantified in pigs at 9, 12 and 21 weeks post infection (wpi) with schistosoma japon ... | 2006 | 17096648 |
| schistosoma japonicum: interactions of successive infections in pigs and mice using polymerase chain reaction-based identification of individual worms. | the study reported here investigated acquired resistance of mice and pigs to challenge-infections with schistosoma japonicum. two morphologically indistinguishable isolates of the parasite (from the anhui and zhejiang provinces of china), which could be typed by polymerase chain reaction-linked restriction fragment length polymorphism analysis (pcr-rflp), were used for the infections. in two parallel infection studies, 60 female outbred nmri mice and 29 danish landrace/yorkshire/duroc crossbred ... | 2000 | 10980295 |
| hepatic changes in congenital schistosoma japonicum infections in pigs. | the inflammatory response in liver tissue from piglets congenitally infected with schistosoma japonicum was examined at two different timepoints after infection. the piglets, which were the offspring of three sows infected with 9000 s. japonicum cercariae in the 10th week of gestation, were allocated into two groups (n=9 and 17) killed 5 or 11 weeks after birth, respectively. all piglets developed a low level infection,with no significant difference between the groups. inflammatory lesions in th ... | 2007 | 17459408 |
| prevalence of schistosoma japonicum infection among animals in fifty villages of samar province, the philippines. | in the philippines, there is a need to understand the contribution of different domestic and wild animals in transmitting schistosoma japonicum infection to humans better. the current study describes variation in animal s. japonicum prevalence across 50 endemic villages of samar province, the philippines. a total of 50 villages were selected, 25 with predominantly rain-fed farms and 25 with some irrigation system. at least 35 cats, dogs, pigs, and water buffaloes each were randomly selected and ... | 2007 | 17627431 |
| elucidation of schistosoma japonicum population dynamics in pigs using pcr-based identification of individuals representing distinct cohorts. | the study reported here investigated the interactions of successive infections and acquired resistance of pigs to challenge infections of schistosoma japonicum. two morphologically indistinguishable geographical isolates from china (from anhui and zhejiang provinces) were used for the infections. the worms of the two isolates were distinguishable by pcr-linked restriction fragment length polymorphism analysis of the nicotinamide adenine dinucleotide phosphate dehydrogenase i gene of the mitochon ... | 1999 | 10961846 |
| schistosoma japonicum in the pig: a new technique for estimation of intestinal tissue egg counts. | this study introduced a new method for estimating intestinal tissue schistosoma japonicum egg counts, based on scraping of the mucosal layer of different sections of the intestines. twenty-eight danish landrace/yorkshire/duroc crossbred pigs were divided into 3 groups of 15, 5 and 8 pigs, respectively. pigs were fed either a high- or low- protein diet and were infected by an intra-muscular or per-oral route of infection with doses of either 1,000, 1,500 or 3,000 s. japonicum cercariae. the pigs ... | 1999 | 10928357 |
| vasoactive intestinal peptide and substance p-like immunoreactivities in the enteric nervous system of the pig correlate with the severity of pathological changes induced by schistosoma japonicum. | limited studies have shown that in intestinal schistosomosis, the enteric nervous tissue becomes inflamed, disrupted and destroyed by granulomas and peptides, amines and neurofilaments contents are altered. therefore, immunoreactivities of vasoactive intestinal peptide and substance p were correlated to pathological lesions in the large intestine from pigs infected with schistosoma japonicum. ganglia situated within or near granulomas showed ganglionitis, and necrosis of neurons as well as infil ... | 2001 | 11595238 |
| neuronal nitric oxide synthase activity is increased during granulomatous inflammation in the colon and caecum of pigs infected with schistosoma japonicum. | neuronal nitric oxide is a non-adrenergic non-cholinergic neurotransmitter in the enteric nervous system and plays a role in a variety of enteropathies including crohn's and chagas' diseases, ulcerative colitis, diabetes, atrophy and hypertrophy. the content of neuronal nitric oxide synthase (nnos) in the colon and the caecum from pigs infected with schistosoma japonicum was studied using immunohistochemical and histochemical staining for nnos and nicotinamide adenine dinucleotide phosphate diap ... | 2002 | 12171250 |
| pathogenesis of congenital infection with schistosoma japonicum in pigs. | to elucidate aspects of pathogenesis of congenital infections with schistosoma japonicum, 5 danish crossbred sows were infected during late pregnancy with a chinese isolate of s. japonicum, and 17 of their offspring (fetuses and piglets) were examined 7, 20. 34, 54, and 69 days postinfection (pi). organ samples were collected for histopathological examination with emphasis on liver and lung. samples of the corresponding placenta were also collected from fetuses at postmortem examinations. perfus ... | 2002 | 12435150 |
| validation of ultrasonography for hepatic schistosomiasis using a porcine schistosoma japonicum model. | ultrasonography is increasingly used to record hepatosplenic involvement in schistosomiasis. however, questions remain unsolved regarding diagnostic accuracy in mild hepatic disease and under influence of confounders like co-infections. we report on a study to evaluate the suitability of a pig model of schistosomiasis japonica to investigate such problems. nine pigs (12 weeks of age) were infected with 1000 schistosoma japonicum cercariae. together with 10 uninfected controls, they underwent b-m ... | 2003 | 12659969 |
| [prokaryotic expression and characterization of an antigenic gene of adult trichinella spiralis]. | to obtain a purified ts87 gene expression product of adult trichinella spiralis and identify its immunogenicity. | 2003 | 12884582 |
| immune events associated with high level protection against schistosoma japonicum infection in pigs immunized with uv-attenuated cercariae. | the vaccination of radiation-attenuated schistosoma japonicum cercariae can induce effective protection in artiodactyl, but the immune events related to protective immunity are not fully understood. to provide a paradigm for a human recombinant antigen vaccine, we have undertaken a vaccination and challenge experiment in pigs, which was recognized as an appropriate animal model in this type of study because of their similarity to human in immunology, and investigated the relative immune events i ... | 2010 | 20976218 |
| molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from taenia solium. | two novel genes encoding lactate dehydrogenase a (ldha) and b (ldhb) homologues, respectively, were identified from the cdna libraries of adult taenia solium (t. solium). the two deduced amino acid sequences both show more than 50% identity to the homologues for danio rerio, xenopus laevis, schistosoma japonicum, sus scrofa, homo sapiens, et al. the identity of the amino acid sequence between tsldha and tsldhb is 57.4%, and that of the nucleotide sequence is 61.5%. recombinant tsldha homologue ( ... | 2011 | 21331787 |
| multiple vaccinations with uv- attenuated cercariae in pig enhance protective immunity against schistosoma japonicum infection as compared to single vaccination. | abstract: | 2011 | 21663666 |
| the effect of a low level primary schistosoma japonicum infection on establishment of a challenge infection in pigs. | the aim of the study was to investigate the effect of a low-level primary infection of schistosoma japonicum on a challenge infection in pigs. groups of pigs were given either light or heavy primary infections and heavy challenge infections 14 weeks later. one group of pigs served as a challenge control group. challenge infection superimposed on the heavily infected pigs did not result in increased worm burden, faecal and tissue egg counts or igg levels. in contrast, the challenge infection esta ... | 2003 | 12954013 |
| research on calpain of schistosoma japonicum as a vaccine candidate. | vaccine development by the use of calpain of schistosoma japonicum has been tried in our laboratory. we cloned cdna encoding the heavy chain of s. japonicum calpain, and prepared recombinant molecule of a possible vaccine region of the heavy chain. when balb/c mice were immunized with our recombinant calpain of s. japonicum with freund's complete adjuvant, we observed significant reduction in worm burden (41.2% reduction, p<0.05), and also significant anti-fecundity effects. in this sense, calpa ... | 2004 | 15081949 |
| prenatal schistosoma japonicum infection in piglets: effect of repeated exposure of the dams on treatment efficacy and susceptibility to challenge infections. | this study elucidated the fate of prenatal infections in piglets born by dams repeatedly infected before and during pregnancy with schistosoma japonicum. independent variables included repeated infections of the dams and treatment or challenge infection (or both) of the prenatally exposed piglets. dependant variables were worm counts, fecal and tissue egg counts, weight gain, and gross pathological observations. fifteen female piglets (the dams) were included, of which 6 received repeated infect ... | 2004 | 15165065 |
| congenital infection of rabbits with schistosoma japonicum and protective immunity of offspring. | recently congenital infection with schistosoma japonicum (s. japonicum) has been demonstrated in pigs, rabbits, mice and dogs. we explored the rabbit as an animal model for the congenital infection of schistosomiasis japonica and assessed the effect of a congenital s. japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection. | 2004 | 15377423 |
| the miniature pig: a unique experimental model for schistosoma japonicum infection. | as part of a search for good animal models for human schistosomiasis, two miniature pigs of the clawn strain (c-1, c-2) were inoculated percutaneously with 200 schistosoma japonicum cercariae of the chinese strain, and the subsequent infection was monitored parasitologically, pathologically and serologically. egg excretion into feces began at 5 weeks post-infection (p.i.) and became pronounced from 8 weeks to 17-20 weeks p.i. the average number of eggs in 1 g feces of each pig at the peak period ... | 2004 | 15464438 |
| the protective effect of a schistosoma japonicum chinese strain 23 kda plasmid dna vaccine in pigs is enhanced with il-12. | the schistosome integral membrane protein sm/sj23 was initially shown to induce protection in mice as a synthetic peptide vaccine and further, as a plasmid dna vaccine to induce protection in mice, sheep and water buffalo. in this study we asked if we could induce protection against challenge infection in pigs against schistosoma japonicum by vaccinating them with a plasmid dna vaccine encoding the s. japonicum chinese strain 23 kda membrane protein. further, we asked if we could enhance protect ... | 2004 | 15519710 |
| the effect of colostrum on pigs pre-natally or post-natally exposed to schistosoma japonicum. | pre-natal infection of schistosoma japonicum in pigs may prove to be a useful model in shedding light on human pre-natal schistosomiasis. this study describes the effects of immune colostrum on worm burdens, tissue egg counts, liver pathology and crude worm or egg antigen-specific igg and iga responses, in groups of pigs pre-natally, pre-natally + post-natally or post-natally exposed to s. japonicum. results suggest that pre-natal exposure and immune colostrum did not affect the establishment of ... | 2004 | 15552404 |
| immunity to schistosomiasis in animals: an update. | the present paper reviews the available literature on the development of immunity to animal schistosoma infections. the majority of the studies on animal schistosomiasis were performed in cattle and pigs and only schistosoma mattheei, s. bovis and s. japonicum received particular attention, mainly because of their recognized veterinary significance or zoonotic aspect. although it is an accepted fact that acquired resistance to schistosoma is of major importance in the regulation of infection int ... | 2005 | 16138850 |
| transmission of schistosoma japonicum by humans and domestic animals in the yangtze river valley, anhui province, china. | the aim of the present work was to assess the relative contribution to transmission of schistosoma japonicum by humans and domestic animals in two villages in the yangtze river valley in anhui province, china. a cross-sectional survey was conducted to determine the prevalence and intensity of s. japonicum in humans, cattle, water buffaloes, horses, pigs, goats, dogs and cats. additionally, for each host species the number of individuals and the mean faecal excretion per day was determined. resul ... | 2005 | 16188215 |
| estimating sensitivity and specificity of a faecal examination method for schistosoma japonicum infection in cats, dogs, water buffaloes, pigs, and rats in western samar and sorsogon provinces, the philippines. | schistosoma japonicum causes a chronic parasitic disease, which persists as a major public health concern in the philippines, the people's republic of china and indonesia. this infection is unique among helminthic zoonoses because it can infect humans and more than 40 other mammals. the objective of this study was to estimate the sensitivity and specificity of the danish bilharziasis laboratory technique in cats, dogs, pigs, water buffaloes and rats in the philippines. faecal samples from each a ... | 2005 | 16188261 |
| schistosoma japonicum triose-phosphate isomerase plasmid dna vaccine protects pigs against challenge infection. | the protective efficacy of a schistosoma japonicum, chinese strain, triose-phosphate isomerase (tpi) plasmid dna vaccine was examined in naïve pigs. pigs were vaccinated with the tpi dna-plasmid alone, or in conjunction with il-12 as pcdna3.1-p35, pcdna3.1-p40 plasmids via intramuscular injection. control pigs were immunized with equivalent amounts of pcdna3.1. pigs were immunized 3 times at 21-day intervals and challenged 30 days after the final boost. forty-five days post-challenge, pigs were ... | 2006 | 16393355 |
| does multiple hosts mean multiple parasites? population genetic structure of schistosoma japonicum between definitive host species. | multi-host parasites, those capable of infecting more than one species of host, are responsible for the majority of all zoonotic, emerging or persistent human and animal diseases and are considered one of the major challenges for the biomedical sciences in the 21st century. we characterized the population structure of the multi-host parasite schistosoma japonicum in relation to its definitive host species by genotyping miracidia collected from humans and domestic animals across five villages aro ... | 2006 | 16876170 |
| cytokine mrna profiles in pigs exposed prenatally and postnatally to schistosoma japonicum. | the pig is a natural host for schistosoma japonicum and a useful animal model of human infection. the aim of the present study was to assess the differences between the cytokine profiles in prenatally or postnatally s. japonicum exposed pigs. seven prenatally exposed pigs, 7 postnatally exposed pigs and 4 uninfected control pigs were compared 27 weeks post exposure. variables included worm burdens, tissue egg counts, liver pathology and mrna levels of il-2, il-4, il-10, il-12, tnf-alpha, tgf-bet ... | 2007 | 17074293 |
| distribution of mast cells in relation to schistosoma japonicum induced lesions in pigs. | the pathogenesis of schistosomiasis japonica has been extensively studied, however only little attention has been paid to the presence and localization of mast cells in relation to schistosoma japonicum induced lesions. the aim of the present pilot study was to assess the parasitological and pathological responses in s. japonicum infected pigs with emphasis on the description of the distribution of mast cells in relation to lesions in the liver and cecum. six pigs were exposed to 2,000 cercariae ... | 2006 | 17121286 |
| real-time pcr for detection of low intensity schistosoma japonicum infections in a pig model. | decades of successful schistosoma japonicum control have increased the interest in how to diagnose low intensity infections. a real-time pcr assay targeting the mitochondrial nadh dehydrogenase i gene in s. japonicum was evaluated in infected pigs with very low egg output. six out of 12 s. japonicum infected pigs were treated with praziquantel 8 weeks after infection and all pigs were followed for 16 weeks post-infection. one commercial and one non-commercial extraction method were evaluated in ... | 2008 | 18036505 |
| multi-host transmission dynamics of schistosoma japonicum in samar province, the philippines. | among the 6.7 million people living in areas of the philippines where infection with schistosoma japonicum is considered endemic, even within small geographical areas levels of infection vary considerably. in general, the ecological drivers of this variability are not well described. unlike other schistosomes, s. japonicum is known to infect several mammalian hosts. however, the relative contribution of different hosts to the transmission cycle is not well understood. here, we characterize the t ... | 2008 | 18215106 |
| proteome approach for identification of schistosomiasis japonica vaccine candidate antigen. | experimental vaccination with radiation-attenuated cercariae (rac) confers possible practical levels of resistance to challenge infection by humoral and by cellular mechanism. here, we aimed to identify possible vaccine antigens by using specific igg antibody from rac vaccinated miniature pig. two milligrams of soluble egg antigen (sea) or schistosomal worm antigen preparation (swap) was fractionated using two dimensional liquid chromatography (proteome pf 2d) consisted of high performance chrom ... | 2009 | 18940265 |
| population genetics of schistosoma japonicum within the philippines suggest high levels of transmission between humans and dogs. | schistosoma japonicum, which remains a major public health problem in the philippines and mainland china, is the only schistosome species for which zoonotic transmission is considered important. while bovines are suspected as the main zoonotic reservoir in parts of china, the relative contributions of various non-human mammals to s. japonicum transmission in the philippines remain to be determined. we examined the population genetics of s. japonicum in the philippines in order to elucidate trans ... | 2008 | 19030225 |
| parasite genetic differentiation by habitat type and host species: molecular epidemiology of schistosoma japonicum in hilly and marshland areas of anhui province, china. | schistosoma japonicum, a parasite of significant public health importance in parts of china and southeast asia, is a true generalist pathogen with over 40 species of mammals suspected as definitive host reservoirs. in order to characterize levels of parasite gene flow across host species and identify the most important zoonotic reservoirs, s. japonicum larvae (miracidia) were sampled from a range of definitive host species in two contrasting habitat types within anhui province, china: a low-lyin ... | 2009 | 19389178 |
| anomalous immunogenic properties of serine proteases. | it has previously been shown that a approximately 27 kda serine protease of schistosoma mansoni larvae, the cercarial elastase (ce), was a poor immunogen in as much as it failed to induce an antibody response. the ce has a critical role in enabling schistosome larvae to penetrate the skin of their definitive hosts, so the apparently poor immunogenicity of this enzyme is clearly of interest. to understand its lack of immunogenicity better and in particular to determine whether it is related to it ... | 2009 | 19751273 |
| prevalences of schistosoma japonicum infection in reservoir hosts in south-western china. | | 2010 | 20406586 |
| characterization and vaccination of two novel schistosoma japonicum genes screened from a cercaria cdna library. | two novel genes, sjcwl05 and sjcwl06, were harvested from screening of schistosoma japonicum (s. japonicum) cercaria cdna library by using pig sera vaccinated (vps) with s. japonicum immature egg ws-vaccine (s. japonicum iew). prokaryotic recombinant plasmids pgex-4t-1/sjcwl05 and pgex-4t-1/sjcwl06 were constructed to analyze their immunogenicity, which was confirmed by sds-page and western blotting. two eukaryotic recombinant plasmids, pcdna3/sjcwl05 and pcdna3/sjcwl06, were constructed, and th ... | 2011 | 21739313 |
| [Antibody responses to leucine aminopeptidase in Schistosoma japonicum infection]. | To investigate the antibody response to leucine aminopeptidase in the different stages of Schistosoma japonicum infection. | 2011 | 22164617 |
| investigation on the epidemiological factors of clonorchis sinensis infection in an area of south china. | to detect the epidemiological factors of clonorchis sinensis infection in hengxian county, one of counties in guangxi zhuang autonomous region where schistosoma japonicum was endemic but eliminated in the late 1980s, a questionnaire was designed with 37 questions covering socioeconomic conditions and human behavior, contamination of the environment and fish ponds, inadequate farming/fishery practices; and the formalin-ether sedimentation technique was used to examine the feces of cats, dogs and ... | 2005 | 16438134 |
| phenotypical characterization of intestinal schistosoma japonicum granulomas in pigs. | the pig has been proposed as a model for human schistosomiasis japonica and the use of this animal model is increasing. the inflammatory response to schistosome infection in the liver and intestine of the pig shows morphological differences, and only the hepatic granulomas have been phenotypically characterized. the aim of the present study was to phenotypically characterize the cellular inflammatory response in the cecum by immunohistochemistry with particular reference to perioval granulomatou ... | 2005 | 16610640 |
| cross-sectional associations between intensity of animal and human infection with schistosoma japonicum in western samar province, philippines. | to estimate the association between the intensity of animal infection with schistosoma japonicum and human infection in western samar province, the philippines. | 2006 | 16799728 |