| polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. | the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ... | 1992 | 1503968 |
| susceptibility of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii to antimicrobial agents used in selective media. | several antimicrobial agents used in selective media for the isolation of arcobacter were found to be inhibitory to strains belonging to this genus. all three species tested were susceptible to colistin and rifampin at concentrations used in selective media. arcobacter skirrowii was the most susceptible species. 5-fluorouracil, novobiocin, trimethoprim, and teicoplanin or vancomycin were found to be without any inhibitory effect on the strains tested at concentrations described for the isolation ... | 2001 | 11283110 |
| determination of the occurrence of arcobacter butzleri in beef and dairy cattle from texas by various isolation methods. | arcobacter butzleri is a pathogenic bacterium that has been found in dairy cattle, pigs, poultry, and humans. as of this writing, there are no data on the incidence of a. butzleri in beef cattle. given the differences in rearing practices used for feedlot cattle and those used for dairy cattle, differences in the incidences of this organism in various types of cattle may also exist. numerous culture methods have been used to isolate a. butzleri, but there are few data on the comparative efficaci ... | 2002 | 12495000 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| isolation of arcobacter butzleri and a. cryaerophilus in samples of meats and from meat-processing plants by a culture technique and detection by pcr. | a pilot survey of sources of contamination with arcobacters (representing a potential risk for humans) was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.) from a retail level and domestic farming. sanitary practices in slaughterhouses and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). the method is based on a selective enrichment to isolate suspect strains, in co ... | 2003 | 12800507 |
| prevalence of arcobacter species in retail meats and antimicrobial susceptibility of the isolates in japan. | a survey was conducted to examine the prevalence of arcobacter species among meat samples and to investigate the antimicrobial susceptibility of the isolates in japan. in 1998 and 1999, samples of beef (n=90), pork (n=100) and chicken meat (n=100) were purchased from seven retail shops. arcobacter species were isolated from 2.2%, 7.0% and 23.0% of beef, pork and chicken meat samples, respectively. the rate of isolations in chicken meats was shown to be significantly higher than those of beef and ... | 2004 | 14751685 |
| isolation and characterisation of arcobacter butzleri from meat. | a survey was conducted to determine the prevalence of arcobacter in ground chicken, pork, beef and lamb meats. meat samples were enriched in arcobacter broth (ab) containing cefoperazone, amphotericin and teicoplanin (cat) supplement. samples were screened for the presence of arcobacter spp. using a multiplex polymerase chain reaction (pcr) followed by isolation on blood and selective agar. arcobacter butzleri was the only species of arcobacter isolated from 35% of 88 samples of ground meats. a. ... | 2004 | 14967558 |
| investigation of arcobacters in meat and faecal samples of clinically healthy cattle in turkey. | to investigate the presence of arcobacter spp. in minced beef meat (n = 97) and rectal faecal samples (n = 200) collected from cattle immediately after slaughter at a local abattoir in turkey. | 2004 | 15214736 |
| prevalence of arcobacter spp. in raw milk and retail raw meats in northern ireland. | a 1-year study was undertaken to determine the prevalence of arcobacter spp. in raw milk and retail raw meats on sale in northern ireland. retail raw poultry samples (n = 94), pork samples (n = 101), and beef samples (n = 108) were obtained from supermarkets in northern ireland, and raw milk samples (n = 101) were kindly provided by the milk research laboratory, department of agriculture and rural development, belfast, northern ireland. presumptive arcobacters were identified by previously descr ... | 2006 | 16924929 |
| detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products. | the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ... | 2006 | 17010041 |
| development of a multiplex and real time pcr assay for the specific detection of arcobacter butzleri and arcobacter cryaerophilus. | a new multiplex pcr and two specific taqman assays were developed to target the emerging pathogens a. butzleri and a. cryaerophilus. the assays also included an internal control to verify the presence of bacterial target dna and amplification integrity. the multiplex assay used a published primer set (cry1 and cry2) for detecting a. cryaerophilus dna (houf, k., tutenel, a., de zutter, l., van hoof, j. and vandamme, p., 2000. development of a multiplex pcr assay for the simultaneous detection and ... | 2007 | 17055091 |
| prevalence and distribution of arcobacter species in various sources in turkey and molecular analysis of isolated strains by eric-pcr. | to determine the prevalence of arcobacter in various food, animal and water sources in turkey and to subtype the isolated strains using enterobacterial repetitive intergenic consensus (eric)-pcr. | 2007 | 17584450 |
| arcobacter contamination on pre- and post-chilled bovine carcasses and in minced beef at retail. | the present study aimed to assess the arcobacter contamination on bovine carcasses postevisceration and postcooling in two slaughterhouses and in ready-to-eat minced beef. | 2010 | 19614853 |
| prevalence of arcobacter species isolated from retail meats in korea. | this study was conducted to determine the prevalence of arcobacter species identified or isolated from retail meats in korea. multiplex pcr assays for the detection of arcobacter species were performed for 360 chicken, 100 pork, and 106 beef samples. arcobacter butzleri and arcobacter cryaerophilus were detected in 18.9 and 3.3% of chicken samples, respectively. however, arcobacter species were not found in any of the pork and beef samples. biochemical testing of isolates selected after enrichme ... | 2010 | 20615344 |
| comparison of arcobacter isolation methods, and diversity of arcobacter spp. in cheshire, united kingdom. | the aims of this study were, firstly, to compare five published methods for the isolation of arcobacter spp. from animal feces in order to determine the most sensitive and specific method. second, we analyzed the resulting isolates by multilocus sequence typing (mlst) in order to investigate the diversity of the isolates recovered. third, we investigated the ability to recover arcobacter spp. from frozen fecal samples. seventy-seven fecal samples from cattle, sheep, and badgers were subjected to ... | 2010 | 21193675 |
| prevalence of arcobacter species in drinking water, spring water, and raw milk as determined by multiplex pcr. | the aim of this study was to investigate the incidence of arcobacter species in water sources and raw milk from healthy animals in kayseri, turkey. a total of 175 samples of drinking water (n = 100), spring water (n = 25), and raw milk (n = 50) were examined. arcobacter species were isolated using the membrane filtration technique. overall, 7 (4%) of the 175 samples yielded arcobacter spp.: 3 (3%) drinking water samples, 1 (4%) spring water sample, and 3 (6%) raw milk samples. two species of arc ... | 2010 | 21219725 |
| molecular analysis and characterization of a urease gene operon from campylobacter sputorum biovar paraureolyticus. | when recombinant plasmid dna from a genomic dna library and inverse pcr products of campylobacter sputorum biovar paraureolyticus lmg17591 strain were analyzed, an approximate 6.5-kb pair region, encoding a urease gene operon, was identified. within the operon, seven closely spaced and putative open reading frames for ureg, ureh(d), urea, ureb, urec, uree, and uref were detected in order. a possible overlap was detected between ureg and ureh(d), ureh(d) and urea, and uree and uref. in addition, ... | 2011 | 21431911 |
| complete genome sequence of the arcobacter butzleri cattle isolate 7h1h. | arcobacter butzleri strain 7h1h was isolated in the united kingdom from the feces of a clinically healthy dairy cow. the genome of this isolate was sequenced to completion. here, we present the annotation and analysis of the completed 7h1h genome, along with a comparison of this genome to the existing a. butzleri genomes. | 2013 | 23969057 |
| helicobacteraceae in bulk tank milk of dairy herds from northern italy. | helicobacter pylori is responsible for gastritis and gastric adenocarcinoma in humans, but the routes of transmission of this bacterium have not been clearly defined. few studies led to supposing that h. pylori could be transmitted through raw milk, and no one investigated the presence of other helicobacteraceae in milk. in the current work, the presence of helicobacteraceae was investigated in the bulk tank milk of dairy cattle herds located in northern italy both by direct plating onto h. pylo ... | 2015 | 26090429 |
| arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii circulation in a dairy farm and sources of milk contamination. | even though dairy cows are known carriers of arcobacter species and raw or minimally processed foods are recognized as the main sources of human arcobacter infections in industrialized countries, data on arcobacter excretion patterns in cows and in milk are scant. this study aimed to identify potentially pathogenic arcobacter species in a dairy herd and to investigate the routes of arcobacter transmission among animals and the potential sources of cattle infection and milk contamination. a strat ... | 2015 | 26002896 |
| arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure. | a study was undertaken to determine the prevalence and diversity of species of the genus arcobacter in pig and dairy cattle manure, which led to the identification of strains af1440t, af1430 and af1581. initially identified as arcobacter butzleri based on colony morphology and initial pcr-confirmation tests, analyses of 16s rrna gene sequences of these strains confirmed that they belonged to the genus arcobacter and were different from all known species of the genus. the isolates formed a distin ... | 2015 | 25977280 |
| genotyping and genetic diversity of arcobacter butzleri and arcobacter cryaerophilus isolated from different sources by using eric-pcr from india. | arcobacters are important zoonotic pathogens and are transmitted through food and water. they are implicated in causing enteritis in animals and humans. among the arcobacter species, a wide genetic diversity has been documented, which reflects continuous evolving nature of these pathogens. | 2014 | 25333916 |
| exploring the diversity of arcobacter butzleri from cattle in the uk using mlst and whole genome sequencing. | arcobacter butzleri is considered to be an emerging human foodborne pathogen. the completion of an a. butzleri genome sequence along with microarray analysis of 13 isolates in 2007 revealed a surprising amount of diversity amongst a. butzleri isolates from humans, animals and food. in order to further investigate arcobacter diversity, 792 faecal samples were collected from cattle on beef and dairy farms in the north west of england. arcobacter was isolated from 42.5% of the samples and the diver ... | 2013 | 23405126 |
| genetic characterization of arcobacter isolates from various sources. | arcobacter is getting more attention due to its detection from wide host-range and foods of animal origin. the objective of this study was to determine the prevalence of arcobacter spp. in various sources at farm level and beef retailed in markets in malaysia and to assess the genetic relatedness among them. a total of 273 samples from dairy cattle including cattle (n=120), floor (n=30), water (n=18) and milk (n=105) as well as 148 beef samples collected from retail markets were studied. the ove ... | 2012 | 22739058 |