| phagocytosis of enteropathogenic escherichia coli and candida albicans by lectin-like receptors. | 1. ingestion of enteropathogenic escherichia coli or candida albicans by thioglycollate-elicited macrophages and polymorphonuclear leukocytes was investigated in vitro, 2. goat antiserum against mannose receptors caused about 50% inhibition of e. coli phagocytosis and about 90% inhibition of c. albicans phagocytosis. 3. e. coli and c. albicans uptake was inhibited by about 60% and 98%, respectively, by plating the macrophages onto substrates coated with poly-l-lysine-mannan. further addition of ... | 1992 | 1342822 |
| microbial pathogens from goat mastitis and phage-typing of staphylococcus aureus isolates. | examination of milk from goats yielded 41 strains from 40 clinically affected halves; 15 were staphylococcus aureus, 6 staph. epidermis, 1 streptococcus agalactiae, 2 strept. dysgalactiae, 5 strept. uberis, 2 corynebacterium pyogenes, 3 escherichia coli, 3 pasteurella spp. and 4 mycoplasma spp. one half had dual infection of staph. aureus and strept. dysgalactiae. twenty two of the 297 milk samples from apparently normal halves also harboured pathogens comprising of 9 staph. aureus, 1 strept. ag ... | 1986 | 2945693 |
| nonspecific and candida-specific immune responses in mice suppressed by chronic administration of anti-mu. | cba/j mice were immunosuppressed by repeated administration of goat antibody specific for mu chain of immunoglobulin m (igm) and tested for nonspecific and candida albicans-specific immune responses. immunosuppression was demonstrated by a dramatic reduction in the number of antibody-forming cells in the spleens of anti-mu-treated mice when immunized with sheep erythrocytes, by greatly reduced in vitro responsiveness of both spleen and lymph node lymphocytes from anti-mu-treated mice to lipopoly ... | 1988 | 3141542 |
| ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on candida albicans. | variability in the expression of two different cell surface carbohydrate determinants was examined with two agglutinating immunoglobulin m monoclonal antibodies (h9 and c6) and immunoelectron microscopy during growth of three strains of candida albicans. a single strain of candida parapsilosis did not express either antigen at any time during growth. antigens were detected on the surface of c. albicans by agglutination tests with either h9 or c6 over a 48-h growth period. the difference in speci ... | 1986 | 3510174 |
| antibody levels to candida albicans carbohydrate and major cytoplasmic antigens isolated from standard and patient strains. | a solid phase radioimmunoassay (ria) and elisa were used to detect human antibodies to candida albicans (ca) organisms or purified fractions, namely, carbohydrate-rich fraction (crf) and cytoplasmic peptides (ssf) of ca. igg antibodies to either whole organisms or to crf were found in sera obtained from patients with chronic mucocutaneous candidiasis (cmcc) or digestive candidiasis, as well as in healthy control sera. in patient sera, no correlation between the clinical stage of disease and the ... | 1984 | 6393857 |
| diagnosis of invasive candidiasis by a dot immunobinding assay for candida antigen detection. | a dot immunobinding assay which uses a polyclonal rabbit anti-candida immunoglobulin g as the primary antibody and colloidal gold coated with goat anti-rabbit immunoglobulin g as the secondary antibody for the detection of candida cytoplasmic antigens is described. it was able to detect as little as 1 ng of total candida protein per ml when a cytoplasmic extract of candida albicans was seeded into buffer and 10 ng/ml when the same extract was seeded into pooled human serum. serial serum samples ... | 1993 | 8458945 |
| macrophage inhibitory factor-a3 derived from mycobacterium avium serovar 2 inhibits candidacidal activity of murine peritoneal macrophages. | macrophage inhibitory factor-a3 (mif-a3), a fraction derived from mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from c57bl/6, c57bl/10, c3h/hej and a/j strains of mice. inhibition of candidacidal activity was demonstrated at mif-a3 concentrations ranging from 100-400 micrograms/ml in macrophages without additional stimulators (exception c3h/hej macrophages) and in macrophages additionally stimulated with 200 u/ml interferon-gamma, 100 ng/ml phorbol myristate acetat ... | 1996 | 9008340 |
| experimental candidal mastitis in goats: clinical, haematological, biochemical and sequential pathological studies. | the present study, first of its kind, was conducted with the objectives to understand hitherto little known aspects of candidal mastitis, like its sequential pathology, pathogenesis and clinico-biochemical changes. for this purpose, unilateral intramammary inoculation of 10 goats with candida albicans (1.2 x 10(7) yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and without dissemination of infection to th ... | 1997 | 9646513 |
| purification and properties of proline-rich antimicrobial peptides from sheep and goat leukocytes. | we purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, oabac5alpha and chbac5, to detailed analysis. oabac5alpha and chbac5 were homologous to each other and to bovine bac5. both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. while the peptides remained active against escherichia coli, pseudomonas aeruginosa, bacillus subtilis, and listeria monocytogenes in 100 ... | 1999 | 10417180 |
| a clinical study of airborne allergens in the united arab emirates. | in the past 25 years the united arab emirates (uae) have experienced a socioeconomic boom. the once nomadic bedouin population of al ain, in the emirate of abu dhabi, now lives in modern air-conditioned accommodation, and huge desalination plants have allowed afforestation and farming. | 1999 | 10572581 |
| pig and goat blood as substitutes for sheep blood in blood-supplemented agar media. | in many developing countries sheep and horse blood, the recommended blood supplements in bacteriological media, are not readily available, whereas pig and goat blood are. therefore, this study examined the use of pig and goat blood as potential substitutes for sheep blood in blood-supplemented bacteriologic media commonly used in clinical microbiology laboratories. in general, the growth characteristics and colony morphologies of a wide range of aerobic and anaerobic bacteria and candida albican ... | 2000 | 10655351 |
| development of eia for detection of chlamydia trachomatis in genital specimens. | a double antibody sandwich enzyme immunoassay (eia) for chlamydial antigen detection was developed using a monoclonal antibody against lipopolysaccharide (lps) of chlamydia trachomatis as a coating antibody. polyclonal rabbit antiserum against partially purified antigen from elementary body (eb) antibody and horse-radish peroxidase conjugated goat anti-rabbit antibody were used as the primary and secondary antibody respectively. the developed eia could detect protein of partially purified eb at ... | 2000 | 11023074 |
| evaluation of oxygen-dependent immunodefences of the polymorphonuclear cells of some tropical ruminants. | activation of polymorphonuclear cells (pmns) leads to the formation of superoxide, which is in turn dismutated to h2o2 by superoxide dismutase (sod) and is partly responsible for oxygen-dependent microbicidal activity. however, no comparative information is available on the effect of sod inhibition before pmn activation to allow simulation of the sod defects that are known to occur in some ruminants. this paper attempts to examine the degranulative and phagocytic responses in buffalo, cattle and ... | 2000 | 11305742 |
| a mannose-binding receptor is expressed on human keratinocytes and mediates killing of candida albicans. | human keratinocytes are known to kill candida albicans in vitro, but the mechanism of killing is not yet understood. here, we demonstrate that spontaneous, ultraviolet-b-light-induced, alpha-melanocyte-stimulating-hormone-induced, and interleukin-8-induced candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (man-bsa). a polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was o ... | 2001 | 11511295 |
| anti-candida antibodies in serum and saliva of patients with denture stomatitis. | quantitative measurement of iga and igg antibodies in the serum and saliva of patients with denture stomatitis (stomatitis subprothetica) was the object of this study. | 2002 | 12751686 |
| isolation and characterisation of proline/arginine-rich cathelicidin peptides from ovine neutrophils. | cathelicidins are a family of gene-encoded antimicrobial peptides found in mammals. seven cathelicidin genes have been identified in sheep, but up to now only two variants of one of these predicted peptides (oabac5) have been purified from ovine neutrophils. in this work numerous proline/arginine-rich cathelicidin peptides were purified, including the originally predicted oabac5 and another oabac5 variant. as well as this, the c-terminus of the predicted oabac7.5 and various truncated forms of o ... | 2003 | 14651991 |
| salivary histatins in human deep posterior lingual glands (of von ebner). | human saliva contains a family of low molecular weight histidine-rich proteins, named histatins, characterised by bactericidal and fungicidal activities in vitro against several microbial pathogens, such as streptococcus mutans and candida albicans. they represent a major component of an innate host non-immune defense system. in an earlier study we described the distribution of histatins in the glandular parenchyma of human major salivary glands, confirming that all human major salivary glands a ... | 2006 | 16859632 |
| study of germ tube formation by candida albicans after photodynamic antimicrobial chemotherapy (pact). | due to the augmented number of immunocompromised patients, the infections associated to the pathogen of the genus candida have increased dramatically in the recent years. in order to proliferate, candida albicans can produce a germ tube formation extending from the cells. the germ tube formation is a transition state from budding to hyphal cells, and represents an essential stage for virulence. in this work we studied the effect of the photodynamic antimicrobial chemotherapy (pact), a potential ... | 2007 | 17566757 |
| aloe vera extract reduces both growth and germ tube formation by candida albicans. | due to the increased number of immunocompromised patients, the infections associated with the pathogen of the genus candida have significantly increased in recent years. to grow, candida albicans may form a germ tube extension from the cells, which is essential for virulence. in this work, we studied the effect of crude glycolic extract of aloe vera fresh leaves (20% w/v) on growth and germ tube formation by c. albicans. the c. albicans growth was determined in the presence of different concentr ... | 2011 | 21914003 |