| the phosphotyrosyl phosphatase activator of protein phosphatase 2a. a novel purification method, immunological and enzymic characterization. | a simple, improved procedure for the isolation of the phosphotyrosyl phosphatase activator (ptpa) from rabbit skeletal muscle has been developed. the majority of the protein phosphatase 2a (pp2a) was separated from ptpa at an early stage in the procedure. the procedure yields approximately 1 mg essentially pure ptpa/kg rabbit skeletal muscle; it was also applied to porcine brain and the yeast saccharomyces cerevisiae. the physico-chemical properties of ptpa obtained from all sources are very sim ... | 1994 | 7813481 |
| induced acute ruminal acidosis in goats treated with yeast (saccharomyces cerevisiae) and bicarbonate. | ruminal acidosis was induced in twenty-one 10-month-old west african dwarf goats by feeding a suspension of 80 g wheat flour per kg bodyweight (day 0) through a stomach tube. ruminal and systemic acidosis was diagnosed on day 1 in all goats. clinical signs included loss of rumination and appetite, trembling, and watery diarrhoea. the detection of acidic faeces during the first 24h was considered of diagnostic importance. subgroups were treated orally on days 1, 2, and 3 either with 1 g of sodium ... | 1995 | 7572459 |
| microbiology of frozen goat meat and toxin production by bacillus cereus isolated therefrom. | microbial analysis of commercial samples of freshly frozen goat meat and those stored at - 12 degrees c for one week revealed high counts of aerobic bacteria, bacillus cereus, staphylococcus aureus and streptococci. these counts increased with storage. psychrophilic bacteria were higher in number than mesophiles. the counts of salmonella were zero cfu/g in both freshly frozen and stock frozen meat. no yeast or moulds were encountered. mouse tests of crude culture filtrate of b. cereus and prepar ... | 1981 | 6798783 |
| a competitive solid-phase radioimmunoassay for translational factors employing monoclonal antibodies. | monoclonal antibodies produced by the hybridoma techniques were purified by chromatography on deae affi-gel blue, and covalently coupled to affi-gel 10 to purify their antigens. the purified components were used to develop a sensitive competitive radioimmune assay for the quantitative determination of translational factors, as described here with a monoclonal antibody directed against yeast elongation factor 3. antigen was adsorbed to polyvinyl chloride plastic surfaces and a limiting concentrat ... | 1984 | 6386991 |
| cross-reactivity of contact site a to antibody produced against a stage-specific antigen from a phenol/water extract of dictyostelium discoideum. | the stage-specific antigen, gp68 (hirano, t., yamada, h., & miyazaki, t. (1983) biochim. biophys. acta 742, 224-234), was purified from a phenol/water extract of aggregation-competent cells of dictyostelium discoideum by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds). anti-gp68 was produced against purified gp68 which was determined to be homogeneous by silver staining on analysis by sds-polyacrylamide gel electrophoresis. the cross-reactivity of a ... | 1984 | 6204971 |
| immunological studies of rna polymerase ii using antibodies to subunits of drosophila and wheat germ enzyme. | we induced goat antibodies to drosophila rna polymerase ii and rabbit antibodies to the isolated 215,000-dalton and 140,000-dalton polymerase ii subunits (p215 and p140, respectively). similarly, we induced rabbit antibodies to wheat germ rna polymerase ii and to the 220,000-dalton subunit and 140,000-dalton subunit (p220 and p140, respectively). anti-polymerase antibodies precipitated the homologous native enzyme and inhibited its activity in vitro, while several of the anti-subunit sera did ne ... | 1982 | 6175634 |
| [enzymatic determination of nonesterified fatty acids in goat plasma]. | two enzymatic methods (wako and nippon shoji kaisha (nsk) for non-esterified fatty acid determination in ruminant blood plasma are tested and compared with a semiautomated conventional chemical method which is based on the measurement of complex extinction made with zinc dibenzyldithiocarbamate. a modification of enzymatic methods is proposed to improve sensitivity and accuracy for the low nefa concentration in ruminant and especially in goat. thus, in this procedure, with 100 microliters of pla ... | 1984 | 6091524 |
| preservation of viable cells in the undercooled state. | previous studies into the mechanisms governing the freezing of cells in the absence of extracellular ice have been extended to develop a method for the preservation of viable cells in the undercooled state. deep undercooling of cells is achieved by suspending fine droplets of the cells in oil to make an emulsion, thus minimizing initiation of extracellular ice nucleation. attempts to preserve yeast cells, cultured sainfoin cells, and dissected shoot-tips (pea and potato) in this way are describe ... | 1985 | 3907980 |
| identification of a shrimp-derived allergen as trna. | during an attempt to isolate shrimp allergens, evidence was obtained that shrimp ribonucleic acid was capable of eliciting a specific ige response in man and an experimental animal model system. the shrimp ribonucleic acid was extracted from boiled whole shrimp (peneaus indicus), and was isolated by salt precipitation and sequential chromatography over deae-sephacel and biogel p-100. the allergenic material was identified as a ribonucleic acid based on the following criteria: a maximal absorptio ... | 1987 | 3584974 |
| evaluation of immuno-dot-blot assay for detection of cholera-related enterotoxin antigen in salmonella typhimurium. | twenty-five strains of salmonella typhimurium isolated in india were examined for the presence of cholera/coli-related enterotoxin antigen by a previously described latex particle agglutination test and by a newly developed immuno-dot-blot test using immunopurified goat antibody against the cholera-related enterotoxin isolated from an escherichia coli strain of human origin. the immuno-dot-blot assay could detect 0.02 ng of purified enterotoxin. the amount of toxin antigen detected varied widely ... | 1987 | 3553233 |
| ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on candida albicans. | variability in the expression of two different cell surface carbohydrate determinants was examined with two agglutinating immunoglobulin m monoclonal antibodies (h9 and c6) and immunoelectron microscopy during growth of three strains of candida albicans. a single strain of candida parapsilosis did not express either antigen at any time during growth. antigens were detected on the surface of c. albicans by agglutination tests with either h9 or c6 over a 48-h growth period. the difference in speci ... | 1986 | 3510174 |
| assessment of different selective agar media for enumeration and isolation of listeria from dairy products. | different selective agar media were compared for the recovery and isolation of five species of listeria from raw milk and cheese. the selective media examined were beerens medium, macbride medium and that described by dominguez et al. (1984) with 6 mg/l acriflavine, listeria selective agar medium (lsam), and lsam with 12 mg/l acriflavine (lsam x 2a); a non-selective yeast glucose lemco agar was included for comparison. when the difference between listeria and the natural microflora of raw milk a ... | 1988 | 3235719 |
| release of a lectin from a fatty acid auxotroph of saccharomyces cerevisiae grown in presence of oleic acid. | the unsaturated fatty acid-requiring mutant kd 115 of saccharomyces cerevisiae secretes a lectin when grown in presence of oleic acid. this lectin is homogeneous on page at ph 8.3, has an approximate molecular weight of 320,000, pi of 4.2 and contains about 60% sugar. it agglutinates chicken and different mammalian erythrocytes, but lyses rabbit red cells only. it is d-galactose-specific. to our knowledge, this is the first report of a hemagglutinin from yeast. | 1986 | 3085672 |
| conservation of the cellular gene encoding the scrapie prion protein. | the major protein, prp 27-30, in purified preparations of hamster scrapie prions is encoded within the genome of the experimental host. dna sequences related to a prp cdna clone can be detected in a wide variety of organisms under relatively stringent conditions where the only signal generated by hamster or mouse dna corresponds to the prp gene. three hosts for scrapie, goat, sheep and rat gave strong hybridization signals. in addition, three invertebrate dnas reacted with the prp probe, in the ... | 1986 | 2870469 |
| studies on phagocytosis of unopsonized rabbit erythrocytes by human monocytes. | monolayers of freshly isolated human monocytes are known to ingest particulate activators of the human alternative complement pathway. the ingestion of rabbit erythrocytes, er, by human monocytes in serum-free medium was studied. the process is mg2+-dependent and optimum phagocytic activity was obtained at approximately 20 mm mgcl2. preincubation of mononuclear leukocytes increased the number of monocytes ingesting er by at least twofold and this involved de novo protein synthesis, as evidenced ... | 1988 | 2834070 |
| immunological properties of recombinant hbsag produced in yeast. | although currently available plasma-derived vaccines (pdv) against hepatitis b based on the surface antigen of the virus (hbsag) are well-tolerated and effective, their supply is limited and time-consuming controls are necessary to assess their safety. it is therefore desirable that an alternative source of hbsag be found. recombinant dna technology has provided the possibility of obtaining hbsag in large quantities. however, it is important that a recombinant dna hepatitis b vaccine be not only ... | 1987 | 2446304 |
| 5-methylcytidylic modification of in vitro transcript from the rat identifier sequence; evidence that the transcript forms a trna-like structure. | previous studies showed that the rat identifier sequence, the rodent type 2 alu family, the rabbit c family and the bovine or goat 73 bp repeat show remarkable resemblance with a few specific trna molecules (sakamoto and okada, j. mol. evol. in press). this paper reports 5-methylcytidylic modification of the in vitro transcript from the id sequence, and provides further evidence that the transcript forms a trna-like structure in vitro. fingerprint analysis and oligonucleotide mapping suggested t ... | 1985 | 2414735 |
| complement-dependence of polymorphonuclear yeast cell phagocytosis: a comparison between man and various domestic animals. | the complement-dependence of polymorphonuclear yeast cell phagocytosis of sheep, goat, cattle, horse, dog, pig and man is determined by comparing the opsonizing abilities of untreated sera with complement-inactivated autologous sera at a serum concentration of 2.5% and at different phagocytosis periods. the performed phagocytosis assays only detects incorporated particles and allows for the differentiation of granulocytes with different quantities of phagocytosed particles. in sheep, horse and m ... | 1990 | 2220191 |
| expression and secretion of goat alpha-lactalbumin as an active protein in saccharomyces cerevisiae. | an expression plasmid for goat alpha-lactalbumin in saccharomyces cerevisiae, pska100, was constructed into a shuttle vector, pyg100, by inserting cdna which encodes goat pre-alpha-lactalbumin and two-thirds of the 3'-non-coding region. the goat alpha-lactalbumin was expressed under the yeast glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and terminator of pyg100 and secreted in the growth medium for yeast as a precise mature protein, possessing specific activity essentially the same as ... | 1990 | 2124489 |
| gene synthesis, expression in escherichia coli, purification and characterization of the recombinant bovine acyl-coa-binding protein. | a synthetic gene encoding the 86 amino acid residues of mature acyl-coa-binding protein (acbp), and the initiating methionine was constructed. the synthetic gene was assembled from eight partially overlapping oligonucleotides. codon usage and nucleotides surrounding the atg translation-initiation codon were chosen to allow efficient expression in escherichia coli as well as in yeast. the synthetic gene was inserted into the expression vector pkk223-3 and expressed in e. coli. in maximally induce ... | 1991 | 2064616 |
| influence of fever and flurbiprofen on trypanosome growth. | the administration of flurbiprofen, a potent non-steroidal anti-inflammatory drug (nsaid), to goats infected with trypanosomes resulted in high elevated parasitaemia and suppression of fever. in contrast to goats, rats infected with trypanosomes do not show febrile reactions. therefore, the role of body temperature was investigated with yeast-induced fever in trypanosoma evansi and t. brucei infected rats. these investigations did not support the hypothesis that a high body temperature causes a ... | 1990 | 1969700 |
| assessment of lymphocyte and phagocytic functions in goats treated with glucan. | the effect of glucan, a biological response modifier of yeast origin, on different immune functions was studied after the intramuscular application in goats. the simultaneous administration of glucan with human serum albumin or tetanus toxoid significantly stimulated the antibody production to both antigens differing in their thymus dependency. similarly, the phagocytizing activity of the blood polymorphonuclear leukocytes measured by the reductase colorimetric assay significantly increased one ... | 1991 | 1789024 |
| function and evolutionary conservation of distinct epitopes on the leukocyte adhesion molecule-1 (tq-1, leu-8) that regulate leukocyte migration. | the leukocyte adhesion molecule-1 (lam-1, tq=1, leu-8) in humans, like its murine homologue, mel-14, is the principal receptor that mediates the binding of leukocytes to high endothelial venules (hev) of peripheral lymph nodes. in this study, several regions of the protein which mediate receptor function were identified by using a large panel of murine mab reactive with lam-1. individual mab reacted with lam-1+ cells with characteristic intensities of immunofluorescence staining, and each bound ... | 1991 | 1713609 |
| ruminal lactic acidosis in sheep and goats. | the clinical findings in 37 sheep and goats with acute ruminal lactic acidosis included a disturbed general condition characterised by anorexia, apathy, teeth grinding and muscle twitching, ruminal stasis, and the excretion of soupy or watery faeces. the ruminal fluid of affected animals was milky, had a sour odour and a low ph. there was a predominance of gram-positive bacteria in smears of ruminal fluid. in comparison with 10 control animals, the rumen fluid of 23 sheep with ruminal lactic aci ... | 1992 | 1595174 |
| influence of yeast (saccharomyces cerevisiae as yea-sacc or levaferm) on in sacco dry matter degradability and ruminal parameters of variously fed small ruminants. | two series of experiments with rumen fistulated castrated male sheep and goats were carried out. in experiment i three sheep each consumed rations rich in concentrate (700 g concentrate, 200 g chopped wheat straw) or roughage (700 g artificially dried ryegrass, 200 g chopped wheat straw per animal per day) and supplemented with 0, 1, 2 or 4 g yea-sacc (saccharomyces cerevisiae; usa) per sheep per day. in experiment ii three sheep were fed with 1000 g artificially dried ryegrass and 200 g concent ... | 1992 | 1338407 |
| clinico-pathological studies on experimental cryptococcal mastitis in goats. | unilateral intramammary inoculation of 10 goats with cryptococcus neoformans (2 x 10(6) yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and there was no dissemination of infection to the opposite uninfected udder halves as well as to other organs of the body. the experiment was continued for 40 days, with 2 animals each from the infected and control groups being killed on 5th, 10th, 20th, 30th and 40th da ... | 1994 | 7935729 |
| a microtiter plate assay for factor xiii a-chain-fibrin interactions. | factor xiii a-chain-fibrin interactions regulate factor xiiia formation and fibrin cross-linking. a microtiter plate assay was developed for studying these interactions. microtiter plate wells were coated with fibrinogen and converted to fibrin by thrombin. after blocking the wells with bovine serum albumin, factor xiii a-chain was added and binding was monitored by incubating first with anti-factor xiii followed by anti-rabbit igg-alkaline phosphatase. enzymatic hydrolysis of p-nitrophenyl phos ... | 1994 | 8059954 |
| an experimental vaccine cocktail for plasmodium falciparum malaria. | surface proteins from several different life-cycle stages of the malaria parasite plasmodium falciparum were expressed at high levels in the yeast saccharomyces cerevisiae. purified proteins, both individually and in cocktails, were used to immunize mice and goats in conjunction with either freund's adjuvant or a muramyl tripeptide-based adjuvant. immune responses were measured by enzyme-linked immunosorbent assays and by the ability of antisera to inhibit (1) the invasion of hepatocytes by live ... | 1993 | 8470430 |
| expression of rat chymotrypsinogen in yeast: a study on the structural and functional significance of the chymotrypsinogen propeptide. | the role of the propeptide sequence and a disulfide bridge between sites 1 and 122 in chymotrypsin has been examined by comparing enzyme activities of wild-type and mutant enzymes. the kinetic constants of mutants devoid of the cys1-cys122 disulfide-linked propeptide show that this linkage is not important either for activity or substrate specificity. however this linkage appears to be the major factor in keeping the zymogen stable against non-specific activation. a comparison of zymogen stabili ... | 1996 | 8635579 |
| fiber-optic fluorometric sensing of polymerase chain reaction-amplified dna using an immobilized dna capture protein. | a fiber-optic assay for amplified dna products has been developed. modifications of the dna capture strategy described previously by kemp et al. [proc. natl. acad. sci. usa 86, 2423-2427 (1989)] were made that allowed selective binding of dna labeled during the amplification process to the sensing surface of fused silica fibers. the gene for a chimeric protein composed of the igg-binding beta 2 subdomain of streptococcal protein g fused with the dna binding domain of yeast gcn4 was constructed, ... | 1996 | 8850548 |
| caprine homologue of rodent 5'-amp-activated protein kinase subunit and yeast snf4/cat3 is down-regulated by thyroid hormone. | we isolated a cdna, b12, that was down-regulated by thyroid hormone (th) in the goat cerebellum, using a polymerase chain reaction (pcr)-based subtractive hybridization and differential screening procedure. northern blot analysis of rna from cerebellum of t4-treated and untreated hypothyroid goats confirmed that clone b12 was th-regulated with an average reduction in expression of 21% after 4 days of t4 supplementation. other tissues from a t4-treated and an untreated hypothyroid goat also revea ... | 1996 | 8872308 |
| caprine mastitis due to aspergillosis and zygomycosis: a pathological and immunohistochemical study. | of 73 goats on a dairy farm, 27 developed mycotic mastitis in the post-partum period. purulent mammary secretion, progressive induration of the affected glands, slight fever and weight loss were observed. as treatment produced no improvement within 2-3 weeks, all diseased animals were slaughtered. at post-mortem examination, widespread acute and chronic mycotic lesions were seen throughout the affected glands. infection was thought to have spread through the milk ducts as an ascending infection ... | 1996 | 8920218 |
| levels and enterotoxigenicity of clostridium perfringens in pozole, tamales, and birria. | a quantitative survey of clostridium perfringens in typical foods served at local restaurants was conducted for 18 months in guadalajara, mexico. a total of 151 samples, including goat's birria (50), pozole (50), and beef tamales (51), were collected from small restaurants in guadalajara. samples were tested for c. perfringens by the most probable number (mpn) method and for mesophilic aerobic plate counts (mapcs) and coliform, yeast, and mold counts by plate count methods. isolates confirmed as ... | 2005 | 15726977 |
| production of therapeutic proteins in the milk of transgenic livestock. | with the advent of the human genome project and associated developments in 'functional genomics', there are going to be increasing numbers of proteins identified and developed for clinical use. there are a number of production methods available, although only three, bacterial, yeast and mammalian cell culture, have produced recombinant proteins that have been approved for clinical use. nevertheless other production systems are under development, and one, the production of human proteins in the m ... | 1998 | 9513718 |
| experimental candidal mastitis in goats: clinical, haematological, biochemical and sequential pathological studies. | the present study, first of its kind, was conducted with the objectives to understand hitherto little known aspects of candidal mastitis, like its sequential pathology, pathogenesis and clinico-biochemical changes. for this purpose, unilateral intramammary inoculation of 10 goats with candida albicans (1.2 x 10(7) yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and without dissemination of infection to th ... | 1997 | 9646513 |
| different regions of rho determine rho-selective binding of different classes of rho target molecules. | based on their rho binding motifs several rho target molecules can be classified into three groups; class i includes the protein kinase pkn, rhophilin, and rhotekin, class ii includes the protein kinases, rho-associated coiled-coil containing protein kinases, rock-i and rock-ii, and class iii includes citron. taking advantage of the selectivity in recognition by these targets between rho and rac, we examined the regions in rho required for selective binding of each class of rho target molecules. ... | 1998 | 9668072 |
| interaction of murine macrophage-membrane proteins with components of the pathogenic fungus histoplasma capsulatum. | the interaction of macrophage-membrane proteins and histoplasmin, a crude antigen of the pathogenic fungus histoplasma capsulatum, was studied using murine peritoneal macrophages. membrane proteins were purified via membrane attachment to polycationic beads and solubilized in tris-hcl/sds/dtt/glycerol for protein extraction; afterwards they were adsorbed or not with h. capsulatum yeast or lectin binding-enriched by affinity chromatography. membrane proteins and histoplasmin interactions were det ... | 1998 | 9737672 |
| assignment1 of the gene of mitochondrial branched chain aminotransferase (bcat2) to sheep chromosome band 14q24 and to cattle and goat chromosome bands 18q24 by in situ hybridization. | | 1998 | 9925942 |
| high-resolution human/goat comparative map of the goat polled/intersex syndrome (pis): the human homologue is contained in a human yac from hsa3q23. | the genetic and cytogenetic map around the chromosome 1 region shown to be linked with polledness and intersexuality (pis) in the domestic goat (capra hircus) was refined. for this purpose, a goat bac library was systematically screened with primers from human coding sequences, scraped chromosome 1 dna, bovine microsatellites from the region, and bac ends. all the bacs (n = 30) were mapped by fluorescence in situ hybridization (fish) on goat chromosome 1q41-q45. the genetic mapping of 30 new goa ... | 1999 | 10036183 |
| quantitating secretion rates of individual cells: design of secretion assays. | to observe events occurring in the microenvironment surrounding individual cells, a mathematical framework has been developed describing the behavior of a compound following its secretion by a single cell. this description is based on the diffusional and binding processes taking place in the vicinity of the cell surface. it allows prediction of the rate of capture and accumulation of a secreted compound around a single cell. this concept provides the basis for the design of two experimental assa ... | 1998 | 10099332 |
| effect of high pressure homogenization on microbial and chemico-physical characteristics of goat cheeses. | the objective of this work was to compare goat cheeses obtained from milk previously subjected to high pressure homogenization (1000 bar) with those produced from untreated milk and milk subjected to sanitization (61 degrees c; 20 min) or to pasteurization (72 degrees c; 15 s). the pressure homogenization treatment had both direct and indirect effects on cheese characteristics and their evolution during ripening. the direct effects were principally linked to the change in water-binding capacity ... | 1999 | 10342224 |
| cryptococcus neoformans and mycobacterium bovis causing granulomatous pneumonia in a goat. | an adult blanca-celtibérica doe originating from a goat herd with a high prevalence of tuberculosis was presented with respiratory signs. at necropsy, this goat had a diffuse and severe mycotic pneumonia associated with the presence of cryptococcus neoformans concomitant with pulmonary focal caseous nodules from which mycobacterium bovis was isolated. microscopically, the mycotic lesion was a granulomatous pneumonia with many large foamy macrophages containing intracellular yeast bodies. the ext ... | 1999 | 10490212 |
| cag repeats in various organisms studied by southern blot analysis. | a 90-nucleotide (cag)30, single-stranded dna was used to probe southern blots in order to indicate the quantity and distribution of long cag repeats in selected genomes. bovine and rat genomes were found to contain a particularly high content of cag repeats, while the repeats were comparatively rare in the human genome. a particularly strong signal in the bovine genome was due to a cag repeat associated with the 1.709 satellite. a similar element was found in goat and musk, but not in the other ... | 1999 | 10495886 |
| microbiological characteristics of anevato: a traditional greek cheese | nine batches of anevato, raw goat milk cheese, were examined throughout a 60 day storage time at three different periods within the lactation season of the goat. high mean log counts per gram of cheese for aerobic bacteria (7.92-9.56), lactic acid bacteria (7. 78-9.32), gram-negative organisms 5.64-9.67), psychrotrophs (7.90-11. 79) and proteolytic bacteria (7.57-9.36) were found. enterobacteriaceae, coliforms and yeasts were considerably lower. enterobacteriaceae and coliforms in the curd of ch ... | 1999 | 10583688 |
| long-term culture and characterization of goat primordial germ cells. | while the culture and identification of primordial germ cells (pgcs) in mice is established, only limited investigations on pgcs in livestock have been reported. this study was performed to characterize goat pgcs after culture and cryopreservation. goat pgcs were isolated from day 32 fetuses and cultured on a continuous cell line of murine embryonal fibroblasts (sto) as feeder-cells in the presence of leukemia inhibitory factor (lif). the pgcs proliferated slowly and showed colony formation in e ... | 2000 | 10798485 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface. | an engineered yeast with emission of fluorescence from the cell surface was constructed. cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (gfp). a glucose-inducible promoter gapdh as a model promoter was selected to control the expression of the reporter gene in response to environmental changes. the gfp gene was fused with the gene encoding the c-terminal half of alpha-agglutinin of saccharomyces cerevisiae having a glycosylphosphatidylinosi ... | 2000 | 10952010 |
| occurrence and characterization of yeasts isolated from milks and dairy products of apulia region. | one hundred and five strains of yeasts isolated from milk of different animal origin and from typical apulian cheeses were studied to identify and characterize yeast strains for further selection as starter cultures for cheese production. the most prevalent isolates belonged to the species trichosporon cutaneum (15.24%), candida catenulata (10.48%) and yarrowia lipolytica (8.57%). in order to evaluate the potential use as starter cultures, the occurrence of some selected properties, such as ferm ... | 2001 | 11589554 |
| yeasts from water buffalo mozzarella, a traditional cheese of the mediterranean area. | countries of the mediterranean area are characterized by production of artisanal cheeses, obtained from goat, sheep, cow and buffalo raw milk. the numbers and species of yeasts in the different cheeses are variable, but some species are more frequently detected than others. kluyveromyces marxianus, k. lactis with their anamorph, candida kefir, debaryomyces hansenii and c. famata, c. colliculosa and c. catenulata are dominant species in several cheeses. however, saccharomyces cerevisiae is often ... | 2001 | 11589559 |
| structural and functional genomics of the cpt1b gene for muscle-type carnitine palmitoyltransferase i in mammals. | muscle-type carnitine palmitoyltransferase i (m-cpt i) is a key enzyme in the control of beta-oxidation of long-chain fatty acids in the heart and skeletal muscle. because knowledge of the mammalian genes encoding m-cpt i may aid in studies of disturbed energy metabolism, we obtained new genomic and cdna data for m-cpt i for the human, mouse, rat, and sheep. the introns of these compact genes are 80% (mouse versus rat) and 60% (mouse versus human) identical. sheep and goat, but not cow, pig, rod ... | 2002 | 12015320 |
| citrinin production and stability in cheese. | citrinin is a nephrotoxic fungal metabolite that has been demonstrated to be mutagenic in hepatocytes. it can be produced by several fungal species that belong mainly to the genus penicillium and has been isolated from many feeds and human foods. cheese is a very sensitive product because it can be naturally contaminated by citrinin-producing molds. the purpose of this study was to determine whether citrinin can be produced in cheeses and whether it is stable in these products. both toxigenic st ... | 2002 | 12182486 |
| pathogenicity of cryptococcus neoformans var. gattii in an immunocompetent mouse model. | the pathogenicity of two different genomic profiles of cryptococcus neoformans var. gattii serotype b isolated from goats that died from cryptococcal pneumonia was assessed in an experimental model of immunocompetent mice. one strain of each randomly amplified polymorphic dna (rapd) profile (gr52 and gr56) and three reference c. neoformans isolates representing serotypes b, d and c were used. balb/c male mice were inoculated by the intraperitoneal route with each strain. after 4 weeks of follow- ... | 2003 | 12627805 |
| immobilization of saccharomyces cerevisiae cells to protein g-sepharose by cell wall engineering. | in this work, we explored the possibility of using the targeting of a heterologous protein to the cell wall of saccharomyces cerevisiae, by fusing it to a cell wall protein, to construct yeast strains whose cells display on their surface proteins that bind to a matrix, so as to achieve the immobilization of the whole cells. with this aim, we created a gene fusion that comprises the region responsible for attachment of a cell wall protein to the cell wall, and the igg binding region of staphyloco ... | 2003 | 12766345 |
| the tat protein of the caprine arthritis encephalitis virus interacts with the notch2 egf-like repeats and the epithelin/granulin precursor. | using the yeast two-hybrid system, we screened a human placenta cdna library and identified two proteins that interacted with the tat protein of the caprine arthritis encephalitis virus (caev): the egf-like repeats 1-6 of the extracellular domain of the human notch2 receptor and the epithelin/granulin growth factor precursor. this interaction was also confirmed in mammalian cells. using in vitro mutagenesis assays, we showed that each one of the three cysteine residues located within the cystein ... | 2003 | 12931033 |
| molecular cloning and expression in yeast of caprine prochymosin. | we cloned and characterized a preprochymosin cdna from the abomasum of milk-fed kid goats. this cdna contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. comparison of the caprine preprochymosin sequence with the corresponding sequences of lamb and calf revealed 99 and 94% identity at the amino acid level. the cdna fragment encoding the mature portion of caprine prochymosin was ... | 2004 | 15464600 |
| preliminary assays for the development of a probiotic for goats. | in order to determine probiotic properties, 137 strains of lactic acid bacteria from the feces of creole goats were screened, only six were resistant to ph 2.0 and bile salts (0.3%). three strains identified as lactobacillus and two as enterococcus showed agglutination with the treated yeast. between them, lactobacillus ddl17, ddl19, ddl48 and enterococcus dde39 demonstrated high specificity in this test because the correspondent agglutination was inhibited by one sugar, suggesting the presence ... | 2004 | 15636159 |
| transfer of an expression yac into goat fetal fibroblasts by cell fusion for mammary gland bioreactor. | yeast artificial chromosomes (yacs) as transgenes in transgenic animals are likely to ensure optimal expression levels. microinjection of yacs is the exclusive technique used to produce yacs transgenic livestock so far. however, low efficiency and high cost are its critical restrictive factors. in this study, we presented a novel procedure to produce yacs transgenic livestock as mammary gland bioreactor. a targeting vector, containing the gene of interest-a human serum albumin minigene (intron 1 ... | 2005 | 15936717 |
| the art and design of genetic screens: mouse. | humans are mammals, not bacteria or plants, yeast or nematodes, insects or fish. mice are also mammals, but unlike gorilla and goat, fox and ferret, giraffe and jackal, they are suited perfectly to the laboratory environment and genetic experimentation. in this review, we will summarize the tools, tricks and techniques for executing forward genetic screens in the mouse and argue that this approach is now accessible to most biologists, rather than being the sole domain of large national facilitie ... | 2005 | 15951745 |
| isolation and characterisation of antimicrobial peptides from deer neutrophils. | the new zealand deer industry is the largest and most advanced in the world. antimicrobial peptides have been isolated from a wide range of organisms, but as yet there have been no reports on any from deer. this work investigates the antimicrobial activity and characterisation of peptides isolated from cervus elaphus blood. it was found that deer blood contains proline/arginine-rich cathelicidins, similar to bac5 peptides isolated from cattle, sheep and goats. a beta-defensin was also isolated t ... | 2005 | 16011891 |
| in situ hybridization by scanning electron microscopy for painting, centromeric, and yac localization. | the hybridization site of a dna probe was detected using a scanning electron microscope (sem), modifying the standard in situ hybridization (ish) method. the experiments were performed on human metaphases obtained from lymphocyte cultures of human peripheral blood. the libraries and probes used were: 1-chromosome library for the painting of chromosome 1 (wcp 1), an alphoid centromere-specific probe of chromosome 8 (pz8.4), and the yeast artificial chromosome (yac) 964-c10 mapped at band p13 on c ... | 2005 | 16079457 |
| angiotensin-converting enzyme inhibitory activity of peptides derived from caprine kefir. | in this study, a potent angiotensin-converting enzyme (ace)-inhibitory activity was found in a commercial kefir made from caprine milk. the low molecular mass peptides released from caseins during fermentation were mainly responsible for this activity. sixteen peptides were identified by hplc-tandem mass spectrometry. two of these peptides, with sequences pyvryl and lvypftgpipn, showed potent ace-inhibitory properties. the impact of gastrointestinal digestion on ace-inhibitory activity of kefir ... | 2005 | 16162521 |
| structure-based ligand binding sites of protein p14.5, a member of protein family yer057c/yil051c/yjgf. | seventeen mutants with one, two or three amino acids substitutions of human protein p14.5, homologue to well-known tumor antigen from goat liver uk114 and a member of proteins yer057c/yil051c/yjgf family, have been used for structure-functional relation studies and ligand binding analysis using cross-linking by triacryloyl-hexahydro-s-triazine (tat), size exclusion chromatography, free fatty acid and 8-anilino-1-naphthalenesulfonic acid (ans) binding assays. amino acids having the most significa ... | 2005 | 16198412 |
| direct labeling of polyphosphate at the ultrastructural level in saccharomyces cerevisiae by using the affinity of the polyphosphate binding domain of escherichia coli exopolyphosphatase. | inorganic polyphosphate (polyp) is a linear polymer of orthophosphate and has many biological functions in prokaryotic and eukaryotic organisms. to investigate polyp localization, we developed a novel technique using the affinity of the recombinant polyphosphate binding domain (ppbd) of escherichia coli exopolyphosphatase to polyp. an epitope-tagged ppbd was expressed and purified from e. coli. equilibrium binding assay of ppbd revealed its high affinity for long-chain polyp and its weak affinit ... | 2005 | 16204477 |
| peptides released from acid goat whey by a yeast-lactobacillus association isolated from cheese microflora. | seven lactobacilli and a variety of microflora extracted from twenty five commercial cheeses were grown on unsupplemented acid goat whey and screened for their capacity to hydrolyse whey proteins [alpha-lactalbumin (alpha-la) and beta-lactoglobulin (beta-lg)] and to generate peptides. fermentations were performed aerobically or anaerobically at 37 degrees c using crude or pre-heated whey (10 min at 65, 75 or 85 degrees c). under aerobic conditions, growth of lactobacilli was poor and protein hyd ... | 2006 | 16476172 |
| kefir: a symbiotic yeasts-bacteria community with alleged healthy capabilities. | kefir is a fermented milk beverage. the milk fermentation is achieved by the of kefir grains, a cluster of microorganisms held together by a polysaccharide matrix named kefiran. kefir grains are an example of symbiosis between yeast and bacteria. they have been used over years to produce kefir, a fermented beverage that is consumed all over the world, although its origin is caucasian. a vast variety of different species of organisms forming the kefir grains, comprising yeast and bacteria, have b ... | 2006 | 16854180 |
| reconstructing prions: fibril assembly from simple yeast to complex mammals. | with the epizootics of bovine spongiform encephalopathy (bse) in north american cattle, bse infections in goats, new forms of human creutzfeldt-jakob disease (cjd) and the spread of chronic wasting disease in north american deer and elk, one wonders whether we are gaining control over the transmissible spongiform encephalopathies (tses). although many basic scientific questions in the prion field remain hotly debated and unresolved [1], including the function of the cellular prion protein (prp), ... | 2005 | 16908997 |
| effect of supplemental yeast culture and sodium bicarbonate on ruminal fermentation and blood variables in rams. | a trial was conducted to evaluate the effect of sodium bicarbonate (bc) and saccharomyces cerevisiae, live yeast culture (yea sacc(1026), ys) on ruminal fermentation and blood variables. four kivircik rams with ruminal cannula were used in a latin square design, during 27-day periods (20 days for adaptation and 7 days for collection). they received 0 (control group), 5 g/day (i.e. 25 x 10(9) cfu) of ys or 10 g/day of sodium bc or 10 g/day of bc and 5 g/day of ys (bc + ys) (treatment groups). the ... | 2006 | 17083424 |
| effect of a soy protein-based diet on ribonucleic acid metabolism in the small intestinal mucosa of goat kids. | this study was designed to investigate the effect of soy protein inclusion in milk replacer diets for goat kids on protein, rna, and dna contents in small intestinal mucosa, on the importance of rna biosynthesis from dietary rna precursors for mucosal rna synthesis, and on the activities of enzymes involved in nucleotide degradation in small intestinal mucosa. diets were based on cow's milk. in the control group, 35% of the milk protein was replaced by casein (cn) protein, and in the soy group ( ... | 2007 | 17430944 |
| monitoring of changes in selenium concentration in goat milk during short-term supplementation of various forms of selenium. | the goal of the experiment was to monitor the changes in the selenium concentration in goat milk during short-term oral supplementation of three different forms of selenium. the experiment involved 24 lactating goats of white shorthaired breed. group c was the control; group s received selenium in the form of selenium-enriched yeast, group l in the form of lactate, and group b in the form of proteinate. individual selenium preparations were administered individually orally in 250 microg se dose ... | 2008 | 17968517 |
| expression and purification of goat lactoferrin from pichia pastoris expression system. | the recombinant goat lactoferrin (rglf) was expressed in the methylotropic yeast pichia pastoris using pgapzalphac vector, gap as promoter, and zeocin as the selective marker. after transformation of the glf-pgapzalphac into pichia pastoris x-33 expression host, the glf-pgapzalphac vector was integrated into the gap promoter locus of pichia pastoris x-33 chromosome. the rglf was expressed and secreted into the broth using alpha-factor preprosequence. sds-page and pas staining analysis indicated ... | 2007 | 17995845 |
| secretion of glycosylated alpha-lactalbumin in yeast pichia pastoris. | the secretion of n-linked glycosylated alpha-lactalbumin was much higher in the expression system of yeast pichia pastoris carrying goat alpha-lactalbumin cdna than in mammalian milk. this is possibly because of the presence of n-linked glycosylation signal sequences, asn(45)-asp(46)-ser(47) and asn(74)-ile(75)-ser(76), in wild-type alpha-lactalbumin. attempts to elucidate the mechanism of the higher secretion of glycosylated alpha-lactalbumin in p. pastoris were made. mutant n45d that deleted t ... | 2002 | 12097163 |
| isolation and characterization of an angiogenin-like protein from goat plasma. | angiogenin, a blood vessel inducing protein has been implicated in wound healing and tumour progression. first isolated from human carcinoma cells, it has been subsequently isolated from human, bovine, rabbit, pig and mouse sera and bovine milk. this study reports the isolation of an angiogenic-like protein from goat plasma. the ribonucleolytic activity has been followed by yeast transfer rna (trna) degradation using spectrophotometric and denaturing polyacrylamide gel electrophoresis methods. t ... | 2002 | 12144504 |
| construction of cell surface-engineered yeasts displaying antigen to detect antibodies by immunofluorescence and yeast-elisa. | in order to detect monoclonal antibodies (mabs) from insufficient and unavailable human proteins, yeast cells were engineered to display human antigens on their surface and consequently endowed with the ability to specifically bind antibodies. thus, a fusion gene for the expression of the human proteasome subunit alpha 6 (hpsa6) and human profilin i (hproi) were assembled, respectively, with a his.tag marker at the c-terminal and displayed on yeast surface. with anti-his.tag mab as the primary a ... | 2008 | 18542951 |
| expression of recombinant hirudin in transgenic mice milk driven by the goat beta-casein promoter. | hirudin, isolated from the leech hirudo medicinalis, inhibits thrombin directly and several expression systems have been used to produce recombinant hirudin (rhirudin) for pharmaceutical purposes. a dna fragment containing the hirudin coding sequence and goat beta-casein secretion signal was chemically synthesized in this study. the synthetic dna then was further constructed into a goat beta-casein expression vector for mouse transgenesis. four lines of transgenic mice were successfully develope ... | 2008 | 18543243 |
| affinity-specific protein separations using ligand-coupled particles in aqueous two-phase systems: ii. recovery and purification of pyruvate kinase and alcohol dehydrogenase from saccharomyces cerevisiae. | the novel approach of using aqueous two-phase systems for the elution of protein from ligand-coupled particles is investigated using pyruvate kinase and alcohol dehydrogenase from recombinant saccharomyces cerevisiae and cibacron blue f3g-a-coupled sepharose cl6b (blue-sepharose) particles as a model system. the ligand-coupled particles distribute quantitatively to the polyethylene glycol-(peg-) rich top phase and the recovered enzymes partition selectively to the dextran-(dex-) rich bottom phas ... | 1989 | 18588025 |
| benomyl and colchicine synergistically inhibit cell proliferation and mitosis: evidence of distinct binding sites for these agents in tubulin. | benomyl, a tubulin-targeted antimitotic antifungal agent, belongs to the benzimidazole group of compounds, which are known to inhibit the binding of colchicine to tubulin. therefore, benomyl was thought to bind at or near the colchicine-binding site on tubulin. however, recent mutational studies in yeast and fluorescence studies involving competitive binding of benomyl and colchicine on goat brain tubulin suggested that benomyl may bind to tubulin at a site distinct from the colchicine-binding s ... | 2008 | 19049291 |
| crude goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus: contribution to proteolysis and ace inhibitory activity. | unsupplemented acid goat whey containing 0.96% protein and 2.76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. these microflora were screened for their ability to hydrolyse whey proteins (alpha-lactalbumin and/or beta-lactoglobulin) and to generate peptides inhibitors of angiotensin i converting enzyme. five microflora were able to degrade whey protein. the most efficient microflora was able to fully hydrolyse alpha-lactalbumin a ... | 2009 | 19121243 |
| molecular epidemiology of isolates of the cryptococcus neoformans species complex from spain. | to study genetic diversity of cryptococcus neoformans species complex in spain, 97 isolates of the yeast recovered from human, animal and environmental samples have been analysed using three molecular epidemiological techniques. one of these, ura5 gene fragment length polymorphism (rflp) analysis, has been previously described as a molecular epidemiology tool. thus, standard profiles and reference strains have been defined for it. in addition, 5s rdna/igs rflp and [gaca](4) microsatellite pcr fi ... | 2009 | 19631160 |
| goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus release tryptophan and tryptophan-lactokinin from a cryptic zone of alpha-lactalbumin. | angiotensin-i-converting enzyme (ace) inhibitors peptides were produced from unsupplemented acid goat whey fermented aerobically for 168 h by kluyveromyces marxianus and lactobacillus rhamnosus. this yeast-lactobacillus association is gras. two novel lactokinins were identified: nyw and w with ic50 of 20 and 0.86 mum respectively. they both were resistant toward simulated gastrointestinal digestion. in addition, wlahk was found in the hydrolysate. these three sequences belong to f(99-110) of alp ... | 2009 | 19650957 |
| elucidating ants in worms using genomic and bioinformatic tools--biotechnological prospects? | adenine nucleotide translocators (ants) belong to the mitochondrial carrier family (mcf) of proteins. atp production and consumption are tightly linked to ants, the kinetics of which have been proposed to play a key regulatory role in mitochondrial oxidative phosphorylation. ants are also recognized as a central component of the mitochondrial permeability transition pore associated with apoptosis. although ants have been investigated in a range of vertebrates, including human, mouse and cattle, ... | 2010 | 19770033 |
| characterization of yeast population and molecular fingerprinting of candida zeylanoides isolated from goat's milk collected in sardinia. | the occurrence of yeast microflora in raw goat's milk collected from 62 dairy farms located in different areas of sardinia was evaluated. candida zeylanoides was the most frequently occurring species followed by different basidiomycetous species. in the strains isolated some biochemical characteristics of technological interest were investigated and a predominance of lipolytic yeast species was found. we employed a simple method of dna extraction that in a minimal time and with low-cost provided ... | 2010 | 19879008 |
| effects of maternal and dietary selenium (se-enriched yeast) on oxidative status in testis and apoptosis of germ cells during spermatogenesis of their offspring in goats. | to investigate the effect of maternal and dietary selenium on antioxidant status in testis and apoptosis of germ cells during spermatogenesis of their offspring, selected taihang black goats (n=119) were randomly allotted to four treatment groups. they were fed the experimental diet with different se levels (from se-enriched yeast) for 174 d from 60 d prior to lactation to weaning of kids. the treatments were: (1) group 1 (control), basal diet without se supplementation, (2) group 2, the same ba ... | 2010 | 20226605 |
| selection of pichia pastoris strains expressing recombinant immunoglobulin g by cell surface labeling. | a simple cell labeling method for sorting yeast pichia pastoris antibody expressing strains is described. a small portion of secreted recombinant antibody retained on the cell surface was labeled with fluorescence detection antibody. the signal intensity of the labeled cell was correlated with the cell's antibody productivity. using this labeling technique to sort a mixture model induced in the same fermenter where the cells of high producing strain were spiked into a population of a low produci ... | 2010 | 20338179 |
| alkaline protease from bacillus cereus vitsn04: potential application as a dehairing agent. | the objective of this work is to use protease enzyme as an ecofriendly alternative to chemicals in dehairing. an alkaline protease producing bacterium was isolated from protein-rich soil sample. the bacterium was identified as bacillus cereus vitsn04 by 16s rrna gene sequencing method. growth characteristics and protease activity were studied in yeast, malt, beef, nutrient broth and soybean casein digest media and the enzyme secretion was found to correspond with growth. maximum protease product ... | 2011 | 20937566 |
| effects of maternal and dietary selenium (se-enriched yeast) on the expression of p34(cdc2) and cyclinb1 of germ cells of their offspring in goats. | the aim of the study was to evaluate the effect of selenium on the expression of p34(cdc2) and cyclinb1 (two components of mpf regulating cell cycle) of germ cells of their offspring in goats. a herd of 119 taihang black goats, which was randomly divided into 4 treatments, received experimental diet with different se levels (from se-enriched yeast) for 174d. the four treatments, fed with a basal diet, were supplemented with 0 (control), 0.5, 2 and 4 mgkg?¹ dm se. testis samples were collected fr ... | 2011 | 21288666 |
| expression of p32 protein of goatpox virus in pichia pastoris and its potential use as a diagnostic antigen in elisa. | the present study was undertaken to express goatpox virus (gtpv) p32 protein in pichia pastoris and evaluate its potential use as a diagnostic antigen in elisa. the amplified p32 gene of gtpv was cloned into ppiczalphaa vector and characterized by pcr, restriction enzyme digestion and sequencing. the characterized linear recombinant plasmids were transformed in pichia host gsii5 strain by electroporation and the zeocin resistant pichia transformant containing p32 gene was selected and confirmed ... | 2009 | 19733197 |
| nutritive composition of tarag, the traditional naturally-fermented goat milk in china. | nutritive compositions were analyzed in 10 batches of samples of tarag (the traditional naturally-fermented goat milk), which were collected in the mongolian community in china. results showed that tarag had richer nutrients than yoghurt. tarag-2 (from zang goats) had better chemical nutrient density than tarag-1 (from chaidamu goats). tarag was rich in casein, lactoferrin, serum albumin, ß-lactoglobulin, a-lactalbumin, and 3 unknown fractions in certain samples. microbiological enumeration reve ... | 2009 | 21883074 |
| fungal diversity in cow, goat and ewe milk. | knowledge of fungal diversity in the environment is poor compared with bacterial biodiversity. in this study, we applied the denaturing high-performance liquid chromatography (d-hplc) technique, combined with the amplification of the its1 region from fungal rdna, for the rapid identification of major fungal species in 9 raw milk samples from cow, ewe and goat, collected at different periods of the year. a total of 27 fungal species were identified. yeast species belonged to candida, cryptococcus ... | 2011 | 21944758 |
| effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristics of low-quality cereal straws. | the effects of yeast culture and fibrolytic enzyme preparation (containing cellulase and xylanase) on in vitro fermentation characteristics of rice straw, wheat straw, maize stover, and maize stover silage were examined using an in vitro gas production technique. four levels of yeast culture and fibrolytic enzyme supplements (0, 2.5, 5.0, and 7.5 g/kg of straw dm, respectively) were tested in a 4 x 4 factorial arrangement. supplementation of yeast culture increased the cumulative gas production, ... | 2008 | 18203979 |
| in this issue: proteomics 3/2008. | in this issue of proteomics you will find the following highlighted articles:once upon a complex busyonce upon a complex busy spied cn pang et al. who found a doozy: proteins partnered for tours of duty and lived lives measured by general utility. all right, enough is enough, back to work. from a variety of studies, particularly in yeast, it is now apparent that specific protein complexes form and disperse as needed or not, in solution or membrane-associated. a common general structure has been ... | 2008 | 18232059 |
| isolation and characterization of human antibodies targeting human aspartyl (asparaginyl) beta-hydroxylase. | over-expression of the enzyme human aspartyl (asparaginyl) beta-hydroxylase (haah) has been detected in a variety of cancers. it is proposed that upon cellular transformation, haah is overexpressed and translocated to the tumor cell surface, rendering it a specific surface antigen for tumor cells. in this work, twelve human single-chain fv fragments (scfv) against haah were isolated from a human non-immune scfv library displayed on the surface of yeast. five of the twelve were reformatted as hum ... | 2007 | 18334751 |
| screening of random peptide library of hemagglutinin from pandemic 2009 a(h1n1) influenza virus reveals unexpected antigenically important regions. | the antigenic structure of the membrane protein hemagglutinin (ha) from the 2009 a(h1n1) influenza virus was dissected with a high-throughput screening method using complex antisera. the approach involves generating yeast cell libraries displaying a pool of random peptides of controllable lengths on the cell surface, followed by one round of fluorescence-activated cell sorting (facs) against antisera from mouse, goat and human, respectively. the amino acid residue frequency appearing in the anti ... | 2011 | 21437206 |
| the effect of various forms of selenium supplied to pregnant goats on the levels of selenium in the body of their kids at the time of weaning. | the aim of this trial was to compare the effect of long-term supplementation of goats with different forms of selenium on body reserves of selenium in their kids at the time of weaning. thirty-three pregnant goats were divided into five groups. group c was control while the other four groups were supplemented with selenium (se) for 6 weeks before parturition (0.3 mg/goat/day) and after parturition (0.9 mg/goat/day). group "se-i" received sodium selenite and three other groups received organic fo ... | 2010 | 21046279 |
| morphology, proliferation, and ribonucleic acid and fractional protein syntheses in the small intestinal mucosa of young goats fed soy protein-based diets with or without amino acid supplementation. | the study was designed to examine whether feeding soy protein isolate as partial replacement of casein (cn) affects jejunal protein synthesis and whether effects may be ameliorated by supplementation of those aa known to be at lower concentrations in soy protein isolate than in cn. goat kids (14 d) were fed comparable milk protein diets, in which 50% of the crude protein was cn (cas), soy protein isolate (spi), or soy protein isolate supplemented with aa (spia) for 43 d (n=8 per group). on d 42, ... | 2010 | 20723691 |