| isolation of an avianlike group a rotavirus from a calf with diarrhea. | an atypical group a rotavirus (993/83) was isolated from a 3-day-old german calf with diarrhea. it differed from 35 conventional german bovine rotavirus isolates analyzed previously with respect to subgroup (strain 993/83 was non-subgroup i and non-subgroup ii), serotype (strain 993/83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and electropherotype (strain 993/83 showed comigrating gene segments 10 and 11). isolate 993/83 reacted with only one ... | 1992 | 1310334 |
| serotyping and antigenic comparison of some animal rotaviruses isolated in china. | eight strains of rotaviruses isolated from diarrheal animals (4 from calves and 4 from piglets) in china were compared by serotyping with reference animal rotavirus strains (bovine ncdv, porcine osu and simian sa-11 and human rotavirus wa strain). two-way cross neutralization test showed no antigenic difference between all 4 local strains of bovine rotavirus (brv007, brv014, hn-7 and brv6555) and reference ncdv, so they belonged to rotavirus serotype 6 (bovine rotavirus serotype 1 or ncdv-seroty ... | 1992 | 1325720 |
| identification of four vp4 serological types (p serotypes) of bovine rotavirus using viral reassortants. | a series of five reassortant viruses each containing the vp4 gene of a distinct bovine rotavirus and the vp7 gene of human rotavirus strain st3 was prepared, and antisera to these were produced in rabbits. in neutralization tests, these antisera allowed the differentiation of the five original strains (from three different vp7 or g serotypes) into three or possibly four vp4 or p serotypes. all of a further seven bovine rotavirus strains adapted to cell culture were successfully typed by these an ... | 1992 | 1328488 |
| comparison of human and porcine group c rotaviruses by northern blot hybridization analysis. | the genetic relationship between human and porcine gp c rotaviruses and between gp c and gp a or b rotaviruses was examined by northern blot hybridization. cross-hybridization studies using radiolabeled ssrna transcript probes demonstrated that the human and porcine gp c rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. neither the human nor the porcine gp c probe hybridized strongly with ... | 1991 | 1648897 |
| protection of agammaglobulinemic piglets from porcine rotavirus infection by antibody against simian rotavirus sa-11. | rotavirus, a double-stranded rna virus, has been implicated as a diarrhea-provoking agent in a variety of animal species. several previous reports have shown that immunization with a single serotype may result in increased in vitro neutralization titers against serotypes not represented in the immunogen. this study was undertaken to determine whether antibody from cows immunized against simian rotavirus strain sa-11 (which is alien to pigs) could protect neonatal piglets from infection with a no ... | 1991 | 1653265 |
| [the serial passage of bovine rotavirus in a heterologous cell culture]. | | 1991 | 1664560 |
| [antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens]. | in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ... | 1991 | 1686823 |
| antigenic and biochemical characterization of bovine rotavirus v1005, a new member of rotavirus serotype 10. | bovine rotavirus (brv) v1005 is serologically distinct from rotavirus serotypes 1, 2, 3, 4, 5, 6, 8 and 9. brv v1005 showed cross-reactions with brv b223, the american prototype of serotype 10 rotavirus, and with brv e4049, a british serotype 10 isolate. brv v1005 was, however, not neutralized by four monoclonal antibodies directed against vp7 of brv b223. two-way cross-reactions were observed between brv v1005 and a reassortant rotavirus containing the vp4 from brv uk. in addition the major try ... | 1990 | 1701478 |
| presence of three p types (vp4 serotypes) and two g types (vp7 serotypes) among bovine rotavirus strains. | cross neutralization tests with a panel of rotavirus strains representing previously described nine vp7 (g) serotypes revealed that bovine rotavirus strain kk-3, a prototype japanese bovine serotype 2, belonged to a new serotype (g10), confirming and extending the recent report of snodgrass et al. [j. clin. microbiol. 28: 504-507 (1990)] which showed that hyperimmune serum to the kk-3 strain neutralized the b223 strain, a proposed type strain of g10. further antigenic characteristics of the kk-3 ... | 1990 | 2175589 |
| effect of mutation in immunodominant neutralization epitopes on the antigenicity of rotavirus sa-11. | exposure of rotavirus sa-11 to polyclonal neutralizing antibody from hyperimmunized guinea-pigs permitted selection of variants which were poorly neutralized by antisera against the parental virus. in one-way cross-neutralization experiments, at least 22 of 24 plaque-purified variants could be classified as belonging to a serotype different from that of the parent. most antisera generated against the variants, however, readily neutralized the parental virus. this indicates that immunodominant ne ... | 1985 | 2414396 |
| isolation of a bovine rotavirus with a "super-short" rna electrophoretic pattern from a calf with diarrhea. | a rotavirus with a "super-short" rna electropherotype was isolated from a calf with diarrhea and was designated vmri strain. segments 10 and 11 of this rotavirus migrated more slowly than did those of bovine rotavirus strains ncdv, b641, and b223. the electrophoretic pattern of the vmri strain was similar to that reported for rotaviruses with super-short rna electropherotypes from humans and rabbits. northern (rna) blot hybridization indicated that gene 11 of the vmri strain was altered and migr ... | 1988 | 2846639 |
| enteric viruses in diarrheic turkey poults. | thirty-three intestinal samples from 10-to-21-day-old diarrheic turkey poults were examined for the presence of enteric viruses by electron microscopy. samples originated from 32 flocks in six commercial operations located in six states. mortality in these flocks ranged from 3 to 15%, and birds from recovered flocks varied greatly in size. rotavirus-like agents (rvla) were the most common viruses associated with diarrhea outbreaks in the flocks examined, occurring in five out of six operations. ... | 1985 | 3000337 |
| genetic relatedness among animal rotaviruses. | the genomic relatedness among representative rotavirus strains was examined by employing cross-hybridization techniques. single stranded (ss) rna prepared by in vitro transcription of purified rotavirus particles and labeled with either 32p or 125i was hybridized to denatured genomic, double stranded (ds) rnas. the hybrids formed were analyzed by polyacrylamide gel electrophoresis (page) or by testing their sensitivity to digestion with single strand specific nuclease (s-1 nuclease). a relativel ... | 1986 | 3004389 |
| enzootic bovine rotavirus is not a source of infection in panamanian cattle ranchers and their families. | vaccination of humans against rotavirus (rv) diarrhea may be accomplished by oral immunization with attenuated animal strains known to be antigenically very similar to human strains. to define better the degree of infectivity in nature of these animal strains for humans, we conducted surveillance for rv infection/diarrhea in 180 farm workers, their 161 family contacts, and the 566 animals (512 cattle, 35 pigs, and 19 sheep) on 14 farms in rural panama. no correlation between the high infection r ... | 1986 | 3009644 |
| a comparison of rotavirus strains of bovine, simian and porcine origin. | three rotavirus strains of bovine, simian and porcine origin, respectively, were compared. the 3 viruses induced a classic rotaviral infection in newborn, conventionally reared calves. the cross neutralization tests revealed an antigenic identity of simian and porcine rotaviruses and a slight serologic correlation of these two viruses with the bovine rotavirus strain. however, in reciprocal cross protection tests carried out in calves, the simian rotavirus antiserum afforded weak protection to c ... | 1985 | 3021518 |
| biochemical evidence for the oligomeric arrangement of bovine rotavirus nucleocapsid protein and its possible significance in the immunogenicity of this protein. | the nucleocapsid protein of bovine rotavirus was shown to exist in trimeric units in both the virus particle and in infected cells, with the subunits linked by non-covalent interactions. these trimeric units complex further by disulphide bridges into larger units which may represent the hexameric structures observed by electron microscopy. visualization of various nucleocapsid protein complexes was also achieved on polyacrylamide gels by treating virus preparations with urea at 37 degrees c or b ... | 1987 | 3027240 |
| immunogenicity and safety of live oral attenuated bovine rotavirus vaccine strain rit 4237 in adults and young children. | a candidate oral live rotavirus vaccine, strain rit 4237, of bovine origin, was tested for immunogenicity and safety in man. in adults the vaccine did not cause clinical symptoms, and a booster response in rotavirus serum antibodies was seen in 2/20 subjects. in seronegative young children one oral dose induced seroconversion to homologous virus in 15/17 (88%) children seronegative by enzyme immunoassay and in 13/19 (68%) children seronegative by a neutralisation assay. the vaccine did not produ ... | 1983 | 6137646 |
| serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. | a total of 16 different strains of rotavirus derived from seven mammalian species (four each from human and porcine species, two each from equine and simian species, and one each from canine and bovine species) and two avian species (one each from turkeys and chickens) were examined in plaque-reduction neutralization tests. seven antigenically distinct serotypes were established on the basis of a greater than or equal to 20-fold difference between titers of homologous and heterologous reciprocal ... | 1984 | 6202807 |
| distribution of antibodies to rotavirus in serum and lacteal secretions of naturally infected swine and their suckling pigs. | rotavirus antibodies were demonstrated in lacteal secretions and sera of 20 parturient sows and in sera of their newborn by an enzyme-linked immunosorbent assay blocking technique, using bovine rotavirus cell culture antigen and monospecific antibody to bovine rotavirus. antibodies to rotavirus occur in the 3 immunoglobulin (ig) classes igm, iga, and igg in lacteal secretions. high and long-persisting antibody activity was mainly associated with the iga class. the igm and igg decreased to undete ... | 1981 | 6267967 |
| isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin. | cytopathic porcine rotavirus was isolated in roller tube cultures of ma-104 cells. faeces of a piglet suffering from diarrhoea, were treated with trypsin which was also added to the maintenance medium. using stationary cultures, virus was not isolated from the same materials. the cytopathic effect was clearly observed after 8 serial passages and the virus titer at the 14th passage level was 10(7) median tissue culture infective doses per ml, or higher. at the 27th passage, rotavirus particles we ... | 1981 | 6271098 |
| a study of cytopathic rotavirus strains isolated from calves with acute enteritis. | nine cytopathic bovine rotavirus strains were isolated in ma-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in central and southern italy. fecal suspensions were treated with a high concentration (1000 micrograms/ml) of trypsin, and inoculated into ma-104 cell cultures grown out in eagle's minimum essential medium (mem) containing 5 micrograms/ml of the enzyme. cyto ... | 1983 | 6313289 |
| protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge. | studies were performed to evaluate the potential use of the bovine rit 4237 rotavirus strain as a vaccine candidate against infection with human rotaviruses. initial experiments revealed that colostrum-deprived piglets were susceptible to infection with several human strains, except for those belonging to subgroup 1. subsequently, different immunization procedures with rit 4237 were studied in this animal model. it was found that a two-dose administration, either given intramuscularly (twice) or ... | 1983 | 6317767 |
| bovine rotavirus type detection by neutralizing monoclonal antibodies. | a series of monoclonal antibodies were developed against bovine rotavirus q17. among the five high affinity antibodies characterized, two (rq 31 and rq 4) were able to neutralize type g 6 viruses and may be specific for pb 1 type virus. seventy seven feces from diarrheic calves were tested by "double sandwich" elisa using four monoclonal and one polyclonal anti-rotavirus antibodies. the combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and ... | 1993 | 7682402 |
| rotavirus interaction with isolated membrane vesicles. | to gain information about the mechanism of epithelial cell infection by rotavirus, we studied the interaction of bovine rotavirus, rf strain, with isolated membrane vesicles from apical membrane of pig enterocytes. vesicles were charged with high (quenching) concentrations of either carboxyfluorescein or calcein, and the rate of fluorophore release (dequenching) was monitored as a function of time after mixing with purified virus particles. purified single-shelled particles and untrypsinized dou ... | 1994 | 8189534 |
| viral determinants of rotavirus pathogenicity in pigs: production of reassortants by asynchronous coinfection. | a porcine rotavirus (prv), variant 4f, isolated in tissue culture from the feces of a chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (j. c. bridger, b. burke, g. m. beards, and u. desselberger, j. gen. virol. 73:3011-3015, 1992). comparison with the closely related pig-apathogenic variant prv 4s suggested the outer capsid protein vp4 (encoded by rna 4) of prv 4f as a determinant for pathogenicity (b. burke, j. c. bridger, and u. desselb ... | 1997 | 8985430 |
| construction of four double gene substitution human x bovine rotavirus reassortant vaccine candidates: each bears two outer capsid human rotavirus genes, one encoding p serotype 1a and the other encoding g serotype 1, 2, 3, or 4 specificity. | previously, four human x bovine rotavirus reassortant candidate vaccines, each of which derived ten genes from bovine rotavirus uk strain and only the outer capsid protein vp7-gene from human rotavirus strain d (g serotype 1), ds-1 (g serotype 2), p (g serotype 3), or st3 (g serotype 4), were developed [midthun et al., (1985): journal of virology 53:949-954; (1986): journal of clinical microbiology 24:822-826]. such human x bovine reassortant vaccines should theoretically provide antigenic cover ... | 1997 | 9093947 |
| bovine rotavirus strain 678 possesses vp4 of serotype p7[5] specificity. | bovine rotavirus strain 678 is the first g8 strain of bovine origin but the literature is confusing as to its p type. in this study, two-way cross neutralization between 678 and 0510, a prototype g6p7[5] virus, was shown by plaque-reduction neutralization assays, establishing the p type of 678 as being p7[5]. the p7[5] specificity of 678 vp4 was reinforced by the finding that the vp8* portion of 678 vp4 had the highest amino acid identity with those of p7[5] bovine rotaviruses. apparent contradi ... | 1997 | 9229013 |
| determinants of rotavirus host range restriction--a heterologous bovine nsp1 gene does not affect replication kinetics in the pig. | the genetic basis of rotavirus host range restriction (host species specificity) is unknown but the nsp1 (fifth) gene has been implicated in some studies. we studied the replication kinetics in vivo of a nsp1 gene monoreassortant, e11, to assess the influence of a heterologous nsp1 gene on the ability to replicate in pigs. the monoreassortant possessed 10 genes from the porcine parent rotavirus sw20/21, which replicated productively in pigs, and the nsp1 gene from the bovine rotavirus uk which p ... | 1998 | 9614866 |
| complete nucleotide sequence of a group a avian rotavirus genome and a comparison with its counterparts of mammalian rotaviruses. | the nucleotide sequences encoding four structural proteins (vp1-4) and six nonstructural proteins (nsp1-6) of avian rotavirus po-13 were determined. based on the results of earlier sequencing studies [ito et al., 1995, sequence analysis of cdna for the vp6 protein of group a avian rota viruses. arch. vriol. 140, 605-612; rohwedder et al., 1997, chicken rotavirus ch-1 shows a second type of avian vp6 gene, virus genes 15, 65-71; rohwedder et al., 1997, bovine rotavirus 993/83 shows a third subtyp ... | 2001 | 11325467 |
| rotavirus cross-species pathogenicity: molecular characterization of a bovine rotavirus pathogenic for pigs. | rotaviruses which cause disease in heterologous animal species have been reported but the molecular basis of cross-species infectivity and disease is not established. we report the molecular characterization of a cloned rotavirus, pp-1, which was originally obtained from cattle and which had been biologically characterized in vivo in two target animal species, gnotobiotic pigs and calves. in pigs, pp-1 caused severe clinical disease but in experimental calves it replicated subclinically. pp-1 wa ... | 2001 | 11878886 |
| generation and characterization of six single vp4 gene substitution reassortant rotavirus vaccine candidates: each bears a single human rotavirus vp4 gene encoding p serotype 1a[8] or 1b[4] and the remaining 10 genes of rhesus monkey rotavirus mmu18006 or bovine rotavirus uk. | the global disease burden of rotavirus diarrhea in infants and young children has stimulated interest in the biological and clinical characteristics of these agents, leading to intensive efforts to develop a vaccine. a rhesus rotavirus (rrv)-based quadrivalent vaccine ("rotashield") was licensed and administered to about 1 million infants and found to be highly effective. however, it was withdrawn because of a link with intussusception. this vaccine was developed according to a modified "jenneri ... | 2002 | 12297404 |
| a rotavirus strain isolated from pig-tailed macaque (macaca nemestrina) with diarrhea bears a p6[1]:g8 specificity. | a distinct rotavirus strain (ptrv) was isolated in cell cultures from a stool sample obtained from a diarrheic 3-year-old female pig-tailed macaque (macaca nemestrina) that was born at the breeding colony of the university of washington in seattle. unlike other known simian rotavirus strains including vervet monkey rotavirus sa11 which bears p5b[2]:g3 or p6[1]:g3 specificity, rhesus monkey rotavirus mmu18006 with p5b[3]:g3 specificity, pig-tailed macaque rotavirus yk-1 with p[3]:g3 specificity a ... | 2006 | 16242747 |
| molecular characterization of novel g5 bovine rotavirus strains. | group a rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. the rotavirus genome is composed of 11 segments of double-stranded rna and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. the aim of this study was to determine if the bovine group a rotavirus strains kj44 and kj75, isolated from clinically infected calves, share genetic features with viruses obtained from hete ... | 2006 | 16928963 |
| zinc-binding domain of rotavirus nsp1 is required for proteasome-dependent degradation of irf3 and autoregulatory nsp1 stability. | interferon regulatory factor 3 (irf3) is a key transcription factor involved in the induction of interferon (ifn) in response to viral infection. rotavirus non-structural protein nsp1 binds to and targets irf3 for proteasome degradation early post-infection. mutational analysis of cysteine and histidine residues within the conserved n-terminal zinc-binding domain in nsp1 of bovine rotavirus strain b641 abolished irf3 degradation in transfected cells. thus, the integrity of the zinc-binding domai ... | 2007 | 17251580 |
| full genome-based classification of rotaviruses reveals a common origin between human wa-like and porcine rotavirus strains and human ds-1-like and bovine rotavirus strains. | group a rotavirus classification is currently based on the molecular properties of the two outer layer proteins, vp7 and vp4, and the middle layer protein, vp6. as reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system that is based on all the rotavirus gene segments is desirable for determining which genes influence rotavirus host range restriction, replication, and virulence, as well as for studying rotavirus epi ... | 2008 | 18216098 |
| [alkaline cultivation of bovine rotavirus in the continuous cell cultures]. | alkaline versus neutral cultivation of bovine rotavirus strain 101 in the continuous cell cultures resulted in a significant acceleration of a reproduction cycle with a very significant cytopathic effect. | 2008 | 18318138 |
| sequence analysis of unusual p[7]g5 bovine rotavirus strains reveals evidence of interspecies transmission. | by sequence and phylogenetic analyses, the 11 genomic segments of two bovine rotaviruses isolated from clinically infected calves were proven to be derived from the swine-like p[7]g5 genotype. this finding reinforced the hypothesis that interspecies transmission of completely heterologous strains can occur in nature. | 2009 | 19710284 |
| diversity and zoonotic potential of rotaviruses in swine and cattle across europe. | group a rotaviruses can infect both humans and animals. individual rotavirus strains can occasionally cross species barriers and might hereby contribute to the emergence of new genotypes in heterologous hosts. the incidence and impact of zoonotic rotavirus are not well defined, and one reason for this is a lack of data about strains circulating in suspected reservoir animal hosts. in this study we report the incidence, genetic diversity, and molecular epidemiology of rotaviruses detected in dome ... | 2011 | 22079216 |