Publications

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congenital bovine epizootic arthrogryposis and hydranencephaly in australia. distribution of antibodies to akabane virus in australian cattle after the 1974 epizootic.at the end of the 1974 epizootic of bovine congenital arthrogryposis and hydranencephaly in south-eastern new south wales, an australia-wide serological survey (about 4,000 serums) was made to determine the ditribution of cattle possessing serum neutralising antibodies against akabane virus. eighty per cent of the serums from cattle in northern australia (western australia, northern territory, and queensland) were positive. a detailed study in the epizootic area in new south wales (particularly ...19761016149
experimental infection of culicoides brevitarsis from south-east queensland with three serotypes of bluetongue virus.laboratory-reared c. brevitarsis (biting midges) were fed on sheep which had been experimentally infected with bluetongue serotype 1 (csiro 156), bluetongue serotype 20 (csiro 19) or bluetongue serotype 21 (csiro 154), or on cattle experimentally infected with bluetongue serotype 20 (csiro 19). approximately 77 000 c. brevitarsis were exposed to sheep and 9000 to cattle. the average percentage feeding on sheep was 54% and on cattle 47%. in attempts to transmit virus by bite 3360 c. brevitarsis w ...19852998313
transmission and in vitro excretion of bluetongue virus serotype 1 by inoculated culicoides brevitarsis (diptera: ceratopogonidae). 19873035181
the isolation of a bluetongue virus from culicoides brevitarsis. 19846331360
infection of culicoides brevitarsis and c. wadai (diptera: ceratopogonidae) with four australian serotypes of bluetongue virus.field collected culicoides brevitarsis kieffer and c. wadai kitaoka were fed on sheep that had been artificially infected with a field-isolate of either bluetongue virus serotype 3 (blu3), blu9, blu16, or blu23. feeding rates averaged 11.9% but were variable. survival of midges during incubation tended to be enhanced by the addition of antibiotics and fungicide to the diet. attempts to transmit virus to sheep by the bite of these two species were unsuccessful. both c. brevitarsis and c. wadai ha ...19948057311
a serological survey for bluetongue virus antibody in western australia.a serological survey was carried out to detect specific (serotype 20) and a group bluetongue virus antibody in cattle and sheep serums collected in western australia during the period january 1 1978 to june 30 1979. of 18,849 cattle serums examined by the gel diffusion precipitin test (gdpt), 9.7% were positive and 6.1% gave doubtful results. all 1949 sheep serums tested were negative. precipitin antibody was demonstrated in 22.5% of serums from kimberley cattle and 3.6% of cattle serums from th ...19806261730
application of an embryonated chicken egg model to assess the vector competence of australian culicoides midges for bluetongue viruses.culicoides biting midges (diptera: ceratopogonidae) are vectors of a number of globally important arboviruses that affect livestock, including bluetongue virus (btv), african horse sickness virus and the recently emerged schmallenberg virus. in this study, a model using embryonated chicken eggs (eces) was utilized to undertake vector competence studies of australian culicoides spp. for 13 laboratory-adapted or wild-type virus strains of btv. a total of 7393 culicoides brevitarsis were reared fro ...201728429824
evaluation of metarhizium anisopliae for the control of culicoides brevitarsis kieffer (diptera: ceratopogonidae), the principal vector of bluetongue virus in australia.four isolates of the entomopathogenic fungus metarhizium anisopliae were tested for their potential to control the biting midge culicoides brevitarsis, the principal vector of bluetongue virus in australia. adult c. brevitarsis died three to eight days after walking on paper substrate treated with 0.7 g/m(2) conidia of any of the isolates, indicating that m. anisopliae has potential as a surface treatment or topical application control strategy. incorporation of the fungus into freshly excreted ...201424820575
assessment of population genetic structure in the arbovirus vector midge, culicoides brevitarsis (diptera: ceratopogonidae), using multi-locus dna microsatellites.bluetongue virus (btv) is a major pathogen of ruminants that is transmitted by biting midges (culicoides spp.). australian btv serotypes have origins in asia and are distributed across the continent into two distinct episystems, one in the north and another in the east. culicoides brevitarsis is the major vector of btv in australia and is distributed across the entire geographic range of the virus. here, we describe the isolation and use of dna microsatellites and gauge their ability to determin ...201526408175
genotyping of whole genome amplified reduced representation libraries reveals a cryptic population of culicoides brevitarsis in the northern territory, australia.the advent of genotyping by next generation sequencing has enabled rapid discovery of thousands of single nucleotide polymorphism (snp) markers and high throughput genotyping of large populations at an affordable cost. genotyping by sequencing (gbs), a reduced representation library sequencing method, allows highly multiplexed sequencing of genomic subsets. this method has limitations for small organisms with low amounts of genomic dna, such as the bluetongue virus (btv) vectors, culicoides midg ...201627716062
population genetic structure and potential incursion pathways of the bluetongue virus vector culicoides brevitarsis (diptera: ceratopogonidae) in australia.culicoides brevitarsis is a vector of the bluetongue virus (btv), which infects sheep and cattle. it is an invasive species in australia with an assumed asian/south east asian origin. using one mitochondrial marker (i.e., part of the cytochrome oxidase subunit i gene) and six nuclear markers, we inferred population genetic structure and possible incursion pathways for australian c. brevitarsis. nine mitochondrial haplotypes, with low nucleotide sequence diversity (0.0-0.7%) among these, were ide ...201626771743
a spatial simulation model for the dispersal of the bluetongue vector culicoides brevitarsis in australia.the spread of bluetongue virus (btv) among ruminants is caused by movement of infected host animals or by movement of infected culicoides midges, the vector of btv. biologically plausible models of culicoides dispersal are necessary for predicting the spread of btv and are important for planning control and eradication strategies.201425105418
the use of discriminant analysis in predicting the distribution of bluetongue virus in queensland, australia.the climatic variables that were most useful in classifying the infection status of queensland cattle herds with bluetongue virus were assessed using stepwise linear discriminant analysis. a discriminant function that included average annual rainfall and average daily maximum temperature was found to correctly classify 82.6% of uninfected herds and 72.4% of infected herds. overall, the infection status of 74.1% of herds was correctly classified. the spatial distribution of infected herds was fou ...19948091641
bluetongue and douglas virus activity in new south wales in 1989: further evidence for long-distance dispersal of the biting midge culicoides brevitarsis.infection of cattle with bluetongue and douglas viruses was detected on the central and southern coast of new south wales from january to april 1989. bluetongue virus infection was found well south of areas of expected occurrence. evidence is presented to support wind-borne dispersal of infected vectors, culicoides brevitarsis, southwards from the hunter valley.19957779035
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