| bokeloh bat lyssavirus isolation in a natterer's bat, poland. | in recent years, bokeloh bat lyssavirus (bblv), a member of the novel lyssavirus genus bokeloh bat lyssavirus in the family rhabdoviridae, has been detected in germany (five cases) and france (two cases). here, we report the isolation of bblv in a natterer's bat (myotis nattereri) in poland. the bat brain tested positive for rabies using classical diagnostics tests (fat and rtcit) and then subsequently confirmed by molecular techniques. viral rna was found in all peripheral organs tested, and th ... | 2018 | 30198169 |
| tentative novel lyssavirus in a bat in finland. | a tentative novel member of the genus lyssavirus, designated as kotalahti bat lyssavirus, was detected in a brandt's bat (myotis brandtii) in finland. based on phylogenetic analysis, the virus differs from other known lyssaviruses, being closely related to khujand virus, aravan virus, bokeloh bat lyssavirus and european bat lyssavirus 2. | 2018 | 29446230 |
| isolation of bokeloh bat lyssavirus in myotis nattereri in france. | bokeloh bat lyssavirus (bblv) was found in myotis nattereri for the first time in northeastern france in july 2012. the complete genome sequence of the virus from the infected natterer's bat was determined by whole-genome sequencing and compared to that of the first bblv strain isolated in 2010 in germany and with those of all currently identified lyssaviruses. the french isolate [kc169985] showed 98.7 % nucleotide sequence identity to the german bblv strain [jf311903]. several organs of the inf ... | 2013 | 23760600 |
| molecular diagnostics for the detection of bokeloh bat lyssavirus in a bat from bavaria, germany. | a brain sample of a natterer's bat tested positive for rabies with classical virological techniques. molecular techniques confirmed the presence of bokeloh bat lyssavirus (bblv) in germany for the second time. sequence analysis revealed a close genetic relationship to the initial german bblv case. using a taqman rt-pcr specific for bblv viral rna was detected in various other organs albeit with differences in the relative viral load. | 2013 | 23932899 |
| lyssavirus distribution in naturally infected bats from germany. | in germany, to date three different lyssavirus species are responsible for bat rabies in indigenous bats: the european bat lyssaviruses type 1 and 2 (eblv-1, eblv-2) and the bokeloh bat lyssavirus (bblv) for which eptesicus serotinus, myotis daubentonii and myotis nattereri, respectively, are primary hosts. lyssavirus maintenance, evolution, and epidemiology are still insufficiently explored. moreover, the small number of bats infected, the nocturnal habits of bats and the limited experimental d ... | 2014 | 24440375 |
| enhanced passive bat rabies surveillance in indigenous bat species from germany--a retrospective study. | in germany, rabies in bats is a notifiable zoonotic disease, which is caused by european bat lyssaviruses type 1 and 2 (eblv-1 and 2), and the recently discovered new lyssavirus species bokeloh bat lyssavirus (bblv). as the understanding of bat rabies in insectivorous bat species is limited, in addition to routine bat rabies diagnosis, an enhanced passive surveillance study, i.e. the retrospective investigation of dead bats that had not been tested for rabies, was initiated in 1998 to study the ... | 2014 | 24784117 |
| bat rabies surveillance in europe. | rabies is the oldest known zoonotic disease and was also the first recognized bat associated infection in humans. to date, four different lyssavirus species are the causative agents of rabies in european bats: the european bat lyssaviruses type 1 and 2 (eblv-1, eblv-2), the recently discovered putative new lyssavirus species bokeloh bat lyssavirus (bblv) and the west caucasian bat virus (wcbv). unlike in the new world, bat rabies cases in europe are comparatively less frequent, possibly as a res ... | 2013 | 22963584 |
| comparative studies on the genetic, antigenic and pathogenic characteristics of bokeloh bat lyssavirus. | bokeloh bat lyssavirus (bblv), a novel lyssavirus, was isolated from a natterer's bat (myotis nattererii), a chiropteran species with a widespread and abundant distribution across europe. as a novel lyssavirus, the risks of bblv to animal and human health are unknown and as such characterization both in vitro and in vivo was required to assess pathogenicity and vaccine protection. full genome sequence analysis and antigenic cartography demonstrated that the german bblv isolates are most closely ... | 2014 | 24828330 |
| evolutionary trends of european bat lyssavirus type 2 including genetic characterization of finnish strains of human and bat origin 24 years apart. | among other lyssaviruses, daubenton's and pond-bat-related european bat lyssavirus type 2 (eblv-2) can cause human rabies. to investigate the diversity and evolutionary trends of eblv-2, complete genome sequences of two finnish isolates were analysed. one originated from a human case in 1985, and the other originated from a bat in 2009. the overall nucleotide and deduced amino acid sequence identity of the two finnish isolates were high, as well as the similarity to fully sequenced eblv-2 strain ... | 2015 | 25877913 |
| molecular epidemiology of bat lyssaviruses in europe. | bat rabies cases in europe are principally attributed to two lyssaviruses, namely european bat lyssavirus type 1 (eblv-1) and european bat lyssavirus type 2 (eblv-2). between 1977 and 2011, 961 cases of bat rabies were reported to rabies bulletin europe, with the vast majority (>97%) being attributed to eblv-1. there have been 25 suspected cases of eblv-2, of which 22 have been confirmed. in addition, two single isolations of unique lyssaviruses from european insectivorous bats were reported in ... | 2013 | 22937876 |
| molecular double-check strategy for the identification and characterization of european lyssaviruses. | the "gold standard" for post-mortem rabies diagnosis is the direct fluorescent antibody test (fat). however, in the case of ante-mortem non-neural sample material or decomposed tissues, the fat reaches its limit, and the use of molecular techniques can be advantageous. in this study, we developed and validated a reverse transcription pcr cascade protocol feasible for the classification of samples, even those for which there is no epidemiological background knowledge. this study emphasises on the ... | 2014 | 24681051 |
| cross-neutralization of antibodies induced by vaccination with purified chick embryo cell vaccine (pcecv) against different lyssavirus species. | rabies is a neglected zoonotic disease caused by viruses belonging to the genus lyssavirus. in endemic countries of asia and africa, where the majority of the estimated 60,000 human rabies deaths occur, it is mainly caused by the classical rabies virus (rabv) transmitted by dogs. over the last decade new species within the genus lyssavirus have been identified. meanwhile 15 (proposed or classified) species exist, including australian bat lyssavirus (ablv), european bat lyssavirus (eblv-1 and -2) ... | 2014 | 25483634 |
| analysis of the complete genome of the first irkut virus isolate from china: comparison across the lyssavirus genus. | the genome of irkut virus, isolate irkv-thchina12, the first non-rabies lyssavirus from china (of bat origin), has been completely sequenced. in general, coding and non-coding regions of this viral genome are similar to those of other lyssaviruses. however, alignment of the deduced amino acid sequences of the structural proteins of irkv-thchina12 with those of other lyssavirus representatives revealed significant variability between viral species. the nucleoprotein and matrix protein were found ... | 2013 | 23872529 |
| [rabies in bats]. | rabies is a zoonosis ending fatally in all mammals, including humans. unlike the other mammals, this disease is usually not fatal in bats. rabies is caused by lyssaviruses which are divided into several distinct phylogroups comprising 15 known viruses. it is believed that the original hosts of all lyssaviruses are bats. classical rabies virus (rabv) occurs in bats across americas and represents the major cause of rabies in humans and domestic animals there. european bat lyssavirus type 1 (eblv-1 ... | 2016 | 27450525 |
| molecular and serological survey of lyssaviruses in croatian bat populations. | rabies is the only known zoonotic disease of bat origin in europe. the disease is caused by species belonging to the genus lyssavirus. five lyssavirus species, i.e., european bat lyssavirus (eblv)-1, eblv-2, bokeloh bat lyssavirus, lleida bat lyssavirus, and west caucasian bat virus, have been identified in european bats. more recently, a proposed sixth species, kotalahti bat lyssavirus, was detected. thus, in this study, active surveillance was initiated in order to obtain insights into the pre ... | 2018 | 30189884 |
| novel lyssavirus in natterer's bat, germany. | a virus isolated from a natterer's bat (myotis nattererii) in germany was differentiated from other lyssaviruses on the basis of the reaction pattern of a panel of monoclonal antibodies. phylogenetic analysis supported the assumption that the isolated virus, bokeloh bat lyssavirus, may represent a new member of the genus lyssavirus. | 2011 | 21801640 |
| the recently discovered bokeloh bat lyssavirus: insights into its genetic heterogeneity and spatial distribution in europe and the population genetics of its primary host. | in 2010, a novel lyssavirus named bokeloh bat lyssavirus (bblv) was isolated from a natterer's bat (myotis nattereri) in germany. two further viruses were isolated in the same country and in france in recent years, all from the same bat species and all found in moribund or dead bats. here we report the description and the full-length genome sequence of five additional bblv isolates from germany (n=4) and france (n=1). interestingly, all of them were isolated from the natterer's bat, except one f ... | 2017 | 29029727 |