rickettsial disease in the peruvian amazon basin. | using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ... | 2016 | 27416029 |
molecular detection of rickettsia felis and candidatus rickettsia asemboensis in fleas from human habitats, asembo, kenya. | the flea-borne rickettsioses murine typhus (rickettsia typhi) and flea-borne spotted fever (fbsf) (rickettsia felis) are febrile diseases distributed among humans worldwide. murine typhus has been known to be endemic to kenya since the 1950s, but fbsf was only recently documented in northeastern (2010) and western (2012) kenya. to characterize the potential exposure of humans in kenya to flea-borne rickettsioses, a total of 330 fleas (134 pools) including 5 species (xenopsylla cheopis, ctenoceph ... | 2013 | 23675818 |
molecular detection of zoonotic rickettsiae and anaplasma spp. in domestic dogs and their ectoparasites in bushbuckridge, south africa. | members of the order rickettsiales are small, obligate intracellular bacteria that are vector-borne and can cause mild to fatal diseases in humans worldwide. there is little information on the zoonotic rickettsial pathogens that may be harbored by dogs from rural localities in south africa. to characterize rickettsial pathogens infecting dogs, we screened 141 blood samples, 103 ticks, and 43 fleas collected from domestic dogs in bushbuckridge municipality, mpumalanga province of south africa, be ... | 2016 | 26974185 |
detection of rickettsia species in fleas collected from cats in regions endemic and nonendemic for flea-borne rickettsioses in california. | rickettsia typhi, transmitted by rat fleas, causes most human flea-borne rickettsioses worldwide. another rickettsia, rickettsia felis, found in cat fleas, ctenocephalides felis, has also been implicated as a potential human pathogen. in the continental united states, human cases of flea-borne rickettsioses are reported primarily from the southern regions of texas and california where the cat flea is considered the principal vector. in california, more than 90% of locally acquired human cases ar ... | 2016 | 26824189 |
first report of a rickettsia asembonensis related infecting fleas in brazil. | the present study was performed in a non-endemic area for spotted fever (sf) in imperatriz microregion, state of maranhão, brazil. blood samples and ectoparasites were collected from 300 dogs of the imperatriz microregion. canine serum samples were tested individually by indirect immunofluorescence assay (ifa), using five rickettsia isolates from brazil. antibodies reactive to at least one of the five species of rickettsia were detected in 1.6% of the dogs (5/300). these sera were considered rea ... | 2017 | 28427962 |
[epidemiological and molecular profile of rickettsiosis in peruvian border locations]. | to determine the circulation of rickettsia in the years 2010 and 2011 in border locations in four regions ofperu and their clinical epidemiological and molecular characteristics. | 2017 | 28538849 |
high prevalence of rickettsia spp. in dog fleas (siphonaptera: pulicidae) in rural uganda. | fleas are known vectors of zoonotic agents. thirty-five fleas, including 28 ctenocephalides felis (bouché), four pulex irritans (l.), and three echidnophaga gallinacea (westwood) from 19 rural dogs from southwestern uganda were analyzed for the presence of rickettsia spp. (ompb, glta, and 17 kda fragment genes) and bartonella spp. (rpob and its genes) by pcr. rickettsial dna was detected in 27 out of 28 of ct. felis and in two out of four p. irritans. none of the e. gallinacea specimens harbored ... | 2017 | 28399271 |
molecular evidence of different rickettsia species in villeta, colombia. | the aim of this work was to detect and identify rickettsia species in ticks collected in rural areas of villeta, colombia. tick specimens were collected from domestic animals and walls of houses in five rural villages of villeta town and from humans in naranjal village (same town). moreover, a flea collected from the same area was also processed. dna was extracted and tested by conventional, semi-nested, and nested pcr reactions targeting rickettsial genes. in the ticks collected from humans fro ... | 2016 | 26789730 |
novel evidence suggests that a 'rickettsia felis-like' organism is an endosymbiont of the desert flea, xenopsylla ramesis. | fleas are acknowledged vectors and reservoirs of various bacteria that present a wide range of pathogenicity. in this study, fleas collected from wild rodents from the negev desert in southern israel were tested for rickettsiadna by targeting the 16s rrna (rrs) gene. thirty-eight xenopsylla ramesis, 91 synosternus cleopatrae and 15 leptopsylla flea pools (a total of 568 fleas) were screened. rickettsiadna was detected in 100% of the x. ramesis and in one s. cleopatrae flea pools. none of l. algi ... | 2015 | 25677805 |
isolation of candidatus rickettsia asemboensis from ctenocephalides fleas. | candidatus rickettsia asemboensis was identified molecularly in fleas collected in 2009 from asembo, kenya. multilocus sequence typing using the 17-kd antigen gene, rrs, glta, ompa, ompb, and sca4 demonstrated that candidatus r. asemboensis is closely related to rickettsia felis but distinct enough to be considered for separate species classification. following this molecular characterization of candidatus r. asemboensis, the in vitro cultivation of this bacterium was then performed. we used cte ... | 2015 | 25897814 |
"candidatus rickettsia asemboensis" in rhipicephalus sanguineus ticks, brazil. | "candidatus rickettsia asemboensis" is an obligate intracellular bacterium of the rickettsiales order, genetically related to species belonging to the rickettsia felis group, agents of flea-borne spotted fever. here we report for the first time the detection of "ca. r. asemboensis", a flea-associated organism, in rhipicephalus sanguineus ticks. it is the first occurrence of this emerging bacterium in brazil, which increases the geographical distribution of this r. felis-like agent. | 2017 | 27986544 |
whole-genome sequence of "candidatus rickettsia asemboensis" strain nmrcii, isolated from fleas of western kenya. | herein we present the draft genome sequence and annotation of "candidatus rickettsia asemboensis" strain nmrcii. "ca. rickettsia asemboensis" is phylogenetically related to but distinct from the flea-borne spotted fever pathogen rickettsia felis. "ca. rickettsia asemboensis" was initially identified in and subsequently isolated from ctenocephalides cat and dog fleas from kenya. | 2015 | 25767219 |