| detection of rickettsia prowazekii in body lice and their feces by using monoclonal antibodies. | in order to identify rickettsia prowazekii in lice, we developed a panel of 29 representative monoclonal antibodies selected from 187 positive hybridomas made by fusing splenocytes of immunized mice with sp2/0-ag14 myeloma cells. immunoblotting revealed that 15 monoclonal antibodies reacted with the lipopolysaccharide-like (lps-l) antigen and 14 reacted with the epitopes of a 120-kda protein. only typhus group rickettsiae reacted with the monoclonal antibodies against lps-l. r. felis, a recently ... | 2002 | 12202579 |
| antigenic classification of rickettsia felis by using monoclonal and polyclonal antibodies. | rickettsia felis is a flea-transmitted rickettsia. there is a discrepancy between its reported phylogenic and phenotypic identifications. following the first report of r. felis, it was considered by tests with serologic reagents to be closely related to another recognized flea-transmitted rickettia, r. typhi. subsequently, it appeared to be more closely related to spotted fever group (sfg) rickettsiae by genetic analysis. in the present work, r. felis was studied by microimmunofluorescence (mif) ... | 2003 | 12626446 |
| [classification and diversity of tick community in tarim basin]. | to investigate the distribution pattern and structural characteristics of tick community and to understand the diversity of the communities in tarim basin. | 2006 | 17366967 |
| [geography and host distribution of crimean-congo hemorrhagic fever in the tarim basin]. | to determine the infective status and natural distribution of xinjiang hemorrhagic fever (xhf; crimean-congo hemorrhagic fever, cchf) in ticks, rodents and livestock in the tarim basin. | 2006 | 17415983 |
| the life cycle of hyalomma asiaticum kozlovi olenev, 1931 (acari: ixodidae) under laboratory conditions. | the developmental stages in the life cycle of hyalomma asiaticum kozlovi were investigated under laboratory conditions. the larval, nymphal and adult ticks were all fed on rabbits at 25-27 degrees c, 50% relative humidity (rh) and exposed to daylight. all free-living stages were maintained in an incubator at 26+/-1 degrees c, 70% rh and daylight conditions. the life cycle of h. asiaticum kozlovi was completed in an average period of 151.6 days (range 104-190). the average developmental periods w ... | 2009 | 19062189 |
| epidemiology and phylogenetic analysis of crimean-congo hemorrhagic fever viruses in xinjiang, china. | in 2004 and 2005, an epidemiological survey of crimean-congo hemorrhagic fever virus (cchfv) was conducted in xinjiang, china. a total of 5,629 serum samples of human and livestock were collected and tested for the cchfv antibody, and 17,319 ticks were collected for viral identification. reverse passive hemagglutination inhibition assays showed that the average prevalence of cchfv antibody was 1.7% for the humans and 12.7% for the livestock. a relatively high antibody prevalence, ranging from 19 ... | 2009 | 19553586 |
| the neglected arboviral infections in mainland china. | the major arboviral diseases in mainland china include japanese encephalitis, dengue fever, crimean-congo hemorrhagic fever (also known as xinjiang hemorrhagic fever), and tick-borne encephalitis. these and other newly found arbovirus infections due to banna virus and tahyna virus contribute to a large and relatively neglected disease burden in china. here we briefly review the literature regarding these arboviral infections in mainland china with emphasis on their epidemiology, primary vectors, ... | 2010 | 20436960 |
| development and evaluation of a loop-mediated isothermal amplification method for rapid detection of anaplasma ovis. | anaplasma ovis is an intraerythrocytic rickettsial pathogen of small ruminants. loop-mediated isothermal amplification (lamp) is a nucleic acid detection method in which the target dna can be efficiently amplified with high specificity and sensitivity under isothermal conditions. in this study, a lamp method was developed for the specific detection of a. ovis, using lamp primers designed on the basis of the major surface protein 4 gene. lamp was performed at 65°c for 30 min. its specificity was ... | 2011 | 21471346 |
| distribution of tick-borne diseases in china. | as an important contributor to vector-borne diseases in china, in recent years, tick-borne diseases have attracted much attention because of their increasing incidence and consequent significant harm to livestock and human health. the most commonly observed human tick-borne diseases in china include lyme borreliosis (known as lyme disease in china), tick-borne encephalitis (known as forest encephalitis in china), crimean-congo hemorrhagic fever (known as xinjiang hemorrhagic fever in china), q-f ... | 2013 | 23617899 |
| emerging tick-borne infections in mainland china: an increasing public health threat. | since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ... | 2015 | 26453241 |
| molecular detection of anaplasma spp. and ehrlichia spp. in ruminants from twelve provinces of china. | anaplasma spp. and ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. there is little information on anaplasmosis and ehrlichiosis in ruminants in china. 16s rrna fret-qpcrs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of china for anaplasma spp. and ehrlichia spp. positive samples were further analyzed with a standard pcr for the glta. anaplasm ... | 2016 | 28096822 |