| an rna-dependent rna polymerase gene of a distinct brazilian tospovirus. | the tospoviral rna-dependent rna polymerases (rdrp), or l proteins, perform several conserved functions during virus replication in host cells. in this study, an l segment sequence of 9,040 bp from a new tospovirus (family bunyaviridae) naturally infecting bean (phaseolus vulgaris l.) plants was characterized. it encodes the largest rdrp gene known yet for this genus, with deduced 2932aa and a molecular mass of approximately 336 kda. a lysine-rich c-terminal extension was found, which apart from ... | 2011 | 21720732 |
| characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the genus tospovirus. | tospoviruses (genus tospovirus, family bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. they have a tripartite negative and ambisense rna genome segments, termed s (small), m (medium) and l (large) rna. the vector-transmission is mediated by thrips in a circulative-propagative manner. for new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history. | 2012 | 22715400 |
| the nsm proteins of phylogenetically related tospoviruses trigger sw-5b-mediated resistance dissociated of their cell-to-cell movement function. | the cell-to-cell movement protein (nsm) of tomato spotted wilt virus (tswv) has been recently identified as the effector of the single dominant sw-5b resistance gene from tomato (solanum lycopersicum l.). although most tswv isolates shows a resistance-inducing (ri) phenotype, regular reports have appeared on the emergence of resistance-breaking (rb) isolates in tomato fields, and suggested a strong association with two point mutations (c118y and t120n) in the nsm protein. in this study the sw-5b ... | 2017 | 28754561 |
| the movement proteins (nsm) of distinct tospoviruses peripherally associate with cellular membranes and interact with homologous and heterologous nsm and nucleocapsid proteins. | tospovirus is the only genus containing virus species which infect plants in the bunyaviridae family. the aims of this study were to understand the in vivo membrane association of the movement protein (nsm) of the tospovirus species bean necrotic mosaic virus, chrysanthemum stem necrosis virus, tomato chlorotic spot virus and tomato spotted wilt virus and the homologous and heterologous interactions among nsm and nucleocapsid protein (n). the results obtained by bimolecular fluorescence compleme ... | 2015 | 25705793 |
| the functional analysis of distinct tospovirus movement proteins (nsm) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species. | the lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. in the present study we have performed a functional analysis of the movement proteins (nsm) of four tospovirus species bean necrotic mosaic virus (benmv), chrysanthemum stem necrosis virus (csnv), tomato chlorotic spot virus (tcsv) and tomato spotted wilt virus (tswv), which differ biologically and molecularly, by using the alfalfa mosaic virus (amv) model sy ... | 2017 | 27697453 |