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capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis.we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ...199910488211
distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil.enrichment cultures on naphtha solvent were used to select aromatic hydrocarbon-degrading bacteria from a btex (benzene, toluene, ethylbenzene, xylene)-contaminated subsoil obtained from beneath a paint factory located in milan, italy. fifteen isolated strains were studied for their different biodegradative capacities. among these, 13 were able to grow on naphtha solvent. ten were identified as pseudomonas putida and three as pseudomonas aureofaciens. two other degraders were identified as pseud ...200011191814
bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus bordetella.a novel bordetella species was isolated from an anaerobic, dechlorinating bioreactor culture enriched from river sediment. the only strain, se-1111r(t) (= dsm 12804t = ccug 43448t), for which the name bordetella petrii is proposed, is designated the type strain of the novel species. strain se-1111r(t) was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. comparative 16s rdna sequence analysis showed a close relationship betw ...200111491321
[amino acid metabolism by thin layer chromatography for the differentiation of bordetella and alcaligenes species].the amino acid metabolism of bordetella and alcaligenes strains was investigated for differentiation purposes. all species investigated reacted differently with a number of amino acids. the genus bordetella could be differentiated from that of alcaligenes by its inability to metabolize l-lysine, l-arginine and d-histidine. the recently described species bordetella avium, as well as achromobacter xylosoxidans (yabuuchi), considered as alcaligenes denitrificans subsp. xylosoxidans, presented thems ...19863788350
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