| identification by polymerase chain reaction (pcr) of marshallagia marshalli and ostertagia gruehneri from svalbard reindeer. | a polymerase chain reaction (pcr) assay to identify two common abomasal nematodes marshallagia marshalli and ostertagia gruehneri of svalbard reindeer was developed. species-specific pcr primers were designed from internal transcribed spacer (its)-2 sequences of rdna and validated using morphologically identified adult male and female nematodes. using the species-specific primers, a 110 bp fragment was amplified from m. marshalli and its minor morph marshallagia occidentalis and a 149 bp fragmen ... | 2000 | 10899533 |