| cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil. | anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ... | 2007 | 17278086 |
| diversity of virulence profiles of shiga toxin-producing escherichia coli serotypes in food-producing animals in brazil. | the prevalence, serotypes and virulence profiles of shiga toxin-producing escherichia coli (stec) were investigated in 205 healthy beef and dairy cattle, and 106 goats reared in the southeastern region of minas gerais state, brazil. the prevalence of stec was 57.5% (61/106) in goats, 39.2%, (40/102) in beef cattle and 17.5% (18/103) in dairy cattle. among the 514 stec isolates, 40 different serotypes were found and some of them were identified in a specific host. stec isolates harboring stx1 cor ... | 2008 | 18678426 |