| emergence of culex pipiens from overwintering hibernacula. | overwintering populations of culex pipiens, the principal enzootic vector of west nile virus in the northeastern usa, were studied over 3 consecutive winters from 2006 to 2008, using mark-recapture techniques to determine when cx. pipiens females began to disperse from overwintering hibernacula and how their survival influenced early season populations. in february of each year, cx. pipiens were aspirated and marked using fluorescent powder; 4,067, 752, and 3,070 diapausing cx. pipiens were mark ... | 2011 | 21476444 |
| west nile virus surveillance in mosquitoes in new york state, 2000-2004. | a west nile virus (wnv) surveillance system was created and implemented in new york state (nys) in 2000 and described previously (white et al. 2001). we examine and evaluate the results of mosquito and virus surveillance for 2000 through 2004 exclusive of new york city. forty-nine counties submitted 1,095,426 mosquitoes in 35,280 pools for wnv assay. specimens of 47 species were tested, with culex species accounting for 47.6% of all pools tested. wnv was detected in 814 pools from 10 species, wi ... | 2006 | 17019772 |
| comparison of vero cell plaque assay, taqman reverse transcriptase polymerase chain reaction rna assay, and vectest antigen assay for detection of west nile virus in field-collected mosquitoes. | mosquitoes collected during the epidemic of west nile virus (wn) in staten island, ny, during 2000 were identified to species, grouped into pools of up to 50 individuals, and tested for the presence of wn by using taqman reverse transcriptase polymerase chain reaction (rt-pcr) to detect west nile viral rna, vero cell plaque assay to detect infectious virus, and vectest wnv/sle antigen panel assay. a total of 10,866 specimens was tested in 801 pools. analysis of results indicated that taqman rt-p ... | 2002 | 12542186 |
| detection of west nile virus-infected mosquitoes and seropositive juvenile birds in the vicinity of virus-positive dead birds. | mosquitoes and wild birds were collected from three sites near locations in the new york city metropolitan area where single, west nile (wn) virus-positive dead birds were found early in the 2000 transmission season. the mosquitoes were tested for the presence of infectious virus with a vero cell culture assay and for wn viral rna by using reverse transcriptase-polymerase chain reaction (rt-pcr) protocols. serum samples from wild birds were tested for the presence of neutralizing antibodies agai ... | 2002 | 12479550 |