| development of a monoclonal blocking elisa for the detection of antibody to mycoplasma bovis in dairy cattle and comparison to detection by pcr. | a monoclonal antibody blocking enzyme-linked immunosorbent assay (b-elisa) was developed to detect antibodies to mycoplasma bovis in cattle sera. the assay was highly specific and sensitive and there was no cross-reaction detected. this method revealed a high prevalence of antibodies (60%) to m. bovis in dairy cattle in north queensland. the diagnostic potential of this b-elisa for the detection of antibody to m. bovis was compared with its detection by pcr. there was a strong positive correlati ... | 2005 | 15734539 |