| an explosive antisense rna strategy for inhibition of a lactococcal bacteriophage. | the coding regions of six putative open reading frames (orfs) identified near the phage phi31 late promoter and the right cohesive end (cos) of lactococcal bacteriophage phi31 were used to develop antisense constructs to inhibit the proliferation of phage phi31. two middle-expressed orfs (orf 1 and orf 2) and four late-expressed orfs (orf 3 through orf 6) were cloned individually between the strong lactobacillus p6 promoter and the t7 terminator (t(t7)) to yield a series of antisense rna transcr ... | 2000 | 10618241 |