| parainfluenza-3 infection of the guinea pig airways induces respiratory airway hyperreactivity in vitro. | guinea pigs were inoculated intra-tracheally with bovine parainfluenza type 3 virus (pi-3) to investigate airway responsiveness to histamine and arecoline in vitro. two days after saline or pi-3 inoculation no difference in the reactivity of the tracheal spirals was observed after cumulative concentration/response curves with both drugs. similarly, contractions of parenchymal strips, induced by histamine, did not differ between both groups. however, 4 days after pi-3 inoculation the histamine in ... | 1990 | 2164762 |
| [a guinea pig model of parainfluenza virus type 3 infection-induced acute and postinfectious cough]. | to establish a guinea pig model of cough induced by human parainfluenza virus type 3 (piv3) infection, and to investigate the change of the cough reflex sensitivity (crs). | 2010 | 21211410 |
| chimeric human parainfluenza virus bearing the ebola virus glycoprotein as the sole surface protein is immunogenic and highly protective against ebola virus challenge. | we generated a new live-attenuated vaccine against ebola virus (ebov) based on a chimeric virus hpiv3/deltaf-hn/ebogp that contains the ebov glycoprotein (gp) as the sole transmembrane envelope protein combined with the internal proteins of human parainfluenza virus type 3 (hpiv3). electron microscopy analysis of the virus particles showed that they have an envelope and surface spikes resembling those of ebov and a particle size and shape resembling those of hpiv3. when hpiv3/deltaf-hn/ebogp was ... | 2009 | 19010509 |
| in vivo and in vitro studies on the use of the guinea pig as a model for virus-provoked airway hyperreactivity. | the parainfluenza 3 (p-3)-infected guinea pig was examined as a model for virus-provoked airway hyperreactivity by measuring changes in airway overflow pressure in response to intravenously (iv) administered histamine. in vitro responses of lung parenchymal strips to several contractile substances were also measured. all studies were conducted 4 days after nasal insufflation with p-3 or p-3 growth medium (control). increases in airway overflow pressure caused by histamine were enhanced by p-3 in ... | 1985 | 2992331 |
| role of sensory neuropeptides in piv-3-infection-induced airway hyperresponsiveness in guinea pigs. | viral respiratory tract infections are known to induce transient airway hyper-responsiveness. the role of the nonadrenergic noncholinergic neuropeptide system on virus-induced airway hyperresponsiveness was studied in the guinea pig. ten guinea pigs were inoculated with parainfluenza 3 virus (piv-3.2 x 10(6) pfu) by nasal route. 16 animals served as untreated controls. viral infection was proven by histological changes and by demonstration of viral antigen using immunohistochemical techniques. f ... | 1997 | 9154673 |
| sensitive plaque neutralization assay for parainfluenza virus types 1, 2, and 3 and respiratory syncytial virus. | a sensitive plaque neutralization assay for parainfluenza virus types 1, 2, and 3 and respiratory syncytial virus was developed in vero and ma 104 cell cultures. the tests were performed in semimicrotoiter trays containing 24 wells, 16 mm in diameter. parainfluenza virus type 1 formed plaques in vero and ma 104 cells only when trypsin was added to the overlay medium. plaquing of parainfluenza virus type 1 was more sensitive and technically reproducible in ma 104 cells than in vero cells. parainf ... | 1981 | 6262372 |