| production and purification of japanese quail ovalbumin as fusion protein with glutathione s-transferase in escherichia coli. | a plasmid encoding a fusion protein interlinked by thrombin recognition sequence between glutathione s-transferase and japanese quail ovalbumin (without 40 amino acid residues from the 5'-end of the orf) has been constructed, employing the expression system pgex-2t. the deglycosylated fusion protein (64 kda) was purified by affinity chromatography on glutathione agarose beads, analyzed by sds-polyacrylamide gel electrophoresis, immunochemically detected with antiserum raised against japanese qua ... | 1995 | 8851561 |
| occurrence of conjunctivitis, sinusitis and upper region tracheitis in japanese quail (coturnix coturnix japonica), possibly caused by mycoplasma gallisepticum accompanied by cryptosporidium sp. infection. | on a farm raising approximately 75,000 japanese quail (coturnix coturnix japonica) for egg production, the diseased quail showed clinical signs of swelling of the head, nasal discharge, increased lacrimation, and decreased egg production. the flock experienced a mortality rate of 5.7% per day. macroscopic observation revealed large, gelatinous masses of caseous exudate in the sinuses, egg peritonitis, and airsacculitis. microscopically, non-purulent or purulent inflammation accompanied by lympho ... | 2002 | 12396337 |
| cellulitis in japanese quail (coturnix coturnix japonica). | a case of cellulitis was observed in japanese quail (coturnix coturnix japonica) reared for commercial meat production. this condition in japanese quail has not been reported in the literature. this incident was the first, and to date only, occurrence of cellulitis in this processing plant. the cellulitis lesions were localized to the subcutis overlying the breast and inner thigh. carcasses of processed birds and live birds from the affected farm were presented to the poultry diagnostic and rese ... | 2003 | 12713180 |
| Effect of polymorphism in egg white lysozyme on muramidase and antibacterial activities as well as hatchability in the Japanese quail (Coturnix japonica). | Lysozyme is one of the best characterized antimicrobial proteins in egg white. Three phenotypes of egg white lysozyme in Japanese quail, Coturnix japonica, (namely F, FS, and S) were observed by acid polyacrylamide gel electrophoresis. The fast (F) phenotype showed faster mobility on Acid-PAGE than the slow (S) phenotype. Comparison of the coding sequences for lysozyme derived from the S and F phenotypes revealed a nonsynonymous single nucleotide polymorphism (SNP) at nucleotide position 115 fro ... | 2011 | 22205671 |
| subchronic lead exposure, immunotoxicology and increased disease resistance in japanese quail (corturnix coturnix japonica). | the present study investigated the effects of lead (pb) on immune responses in quail (coturnix coturnix japonica) and the pathological impact of exposure to an infectious agent (e. coli o2). fifty-four, 4-week-old quail were exposed to lead acetate in drinking water at 5 or 50ppm. all birds were vaccinated with newcastle vaccine (ndv) during the third week of contaminant (pb) exposure. in the fourth week, several arms of the immune response were tested using the t cell based phytohemagglutinin ( ... | 2010 | 21111478 |
| phenotypic and genotypic properties of escherichia coli isolated from colisepticemic cases of japanese quail. | this study was conducted to characterize the escherichia coli isolates from colisepticemic japanese quails. one hundred and nine e. coli were isolated in pure culture from heart blood of dead japanese quails. the sampled birds were originated from four different farms. antibiotic resistance pattern of e. coli isolates were determined against nine antibacterial agents. phylotype and virulence genes of the isolates were detected by polymerase chain reaction. by disc diffusion method, all of the is ... | 2010 | 20401756 |
| [physiologically active argon-based gas mixtures as a means of creating fire-safe gaseous environments in pressurized modules of varying purpose]. | development of acute hypoxic hypoxia in consequence of fire in various pressurized modules was studied. the investigation was aimed to look into the effects of inert argon and dramatic oxygen reduction in gaseous environment on animals (wistar rats, mice c57bl/6, balb/c and f1cbaxbalb/c hybrids, japanese quail embryos) and microorganism (escherichia coli strains k-12 jf 238 and k-12 jm 83). also, inflammability of some widely used materials was assessed in order to provide inputs to developers o ... | 2008 | 18714727 |
| isolation of e. coli from foam and effects of fluoroquinolones on e. coli and foam production in male japanese quail. | sexually active male japanese quail (coturnix coutrnix japonica) produce a foamy substance from their cloacal gland. it was postulated that bacteria played an important role in production of foam. the primary objective of this study was to isolate and identify bacteria present in the cloacal foam. the secondary objective was to evaluate the effect of fluoroquinolone treatment on bacterial counts and foam production. healthy adult japanese quail were maintained in individual cages under uniform h ... | 2004 | 15451247 |
| outbreak of septicaemic colibacillosis in japanese quail (coturnix coturnix japonica). | we have carried out an aetiological and pathological study of an outbreak of septicaemia caused by the o165 serogroup of escherichia coli in japanese quail (coturnix coturnix japonica) which resulted in high mortality (90%). after treatment with amoxicilin in drinking water (200 mg/l), morbility and mortality rates dropped markedly. microbiological studies showed that the organism responsible was an atypical e. coli strain, on the basis of the non-fermentation of lactose (onpg-), which belonged ... | 1999 | 10481623 |
| active immunization of japanese quail hens with a recombinant chicken inhibin fusion protein enhances production performance. | the effects of active immunization against inhibin on production performance in female japanese quail (coturnix coturnix japonica) were assessed in two separate trials using an mbp-cina521 fusion protein as an immunogen. the fusion protein, mbp-cina521, consisted of the bacterial maltose binding protein (mbp) and a truncated form of the mature alpha-subunit of chicken inhibin (cina521). mbp-cina1521 was constructed by: 1) excising a 521-bp psti fragment from a chicken inhibin alpha-subunit cdna ... | 1998 | 9628541 |
| dna in uninfected and virus-infected cells complementary to avian tumor virus rna. | the 70s rna component of several avian tumor viruses was hybridized with dna extracted from avian tumor virus-infected and uninfected chicken and japanese quail cells. tritium-labeled 70s rnas from rous sarcoma virus (rsv), rous associated virus-1 (rav-1), rav-60, and schmidt-ruppin-rsv (sr-rsv) hybridize from 3 to 10 times more with dna from uninfected chicken cells than with dna from escherichia coli, calfthymus, or baby hamster kidney cells. after infection of chicken cells with rsv(rav-1), s ... | 1971 | 4332808 |
| cloning of japanese quail ovalbumin cdna in e. coli. | japanese quail oviduct total cdna was synthesized from total mrna by the classical method, using amv reverse transcriptase, the klenow fragment of dna polymerase i and s1 nuclease. the results of the synthesis of total ss-cdna partially differed from those published for the synthesis of chicken total ss-cdna. the presumed causes of the differences in the complete reverse transcription of mrnacon and incomplete reverse transcription of mrnalys and a large part of mrnaov in our case are discussed. ... | 1989 | 2659390 |
| phylogenetic typing and molecular detection of virulence factors of avian pathogenic escherichia coli isolated from colibacillosis cases in japanese quail. | colibacillosis caused by avian pathogenic escherichia coli (apec) is an economic threat to the poultry industry throughout the world. some of the virulence genes may enhance the ability of e. coli isolates to grow in the tissues of broilers. the apec strains are assigned to a few distinct phylogenetic groups. the purpose of the present study was to detect the virulence genes and phylogenetic groups of e. coli isolates from colibacillosis cases in japanese quail in 2014 in kerman, iran. in the pr ... | 2017 | 28473898 |
| response of restraint stress-selected lines of japanese quail to heat stress and escherichia coli challenge. | japanese quail selected for divergent corticosterone response to restraint stress were evaluated for their susceptibility to heat stress and challenge with escherichia coli. these quail lines are designated as high stress (hs), low stress (ls), and the random-bred control (cs) lines. heat stress (35°c, 8 h/d) began at 24 d until the end of the study at 39 d. birds were challenged with an aerosol spray containing 2 × 10(9) cfu of e. coli at 25 and 32 d. at 38 d, the surviving birds were necropsie ... | 2013 | 23436510 |
| immunomodulation and hormonal disruption without compromised disease resistance in perfluorooctanoic acid (pfoa) exposed japanese quail. | this study evaluated the impact of oral perfluorooctanoic acid (pfoa) on japanese quail at concentrations found in american and belgian workers at pfoa manufacturing facilities. three arms of the immune system were tested; t cell, b cell, and innate immunity. after 6 weeks exposure, quail were challenged with e. coli infection to test the ultimate measure of immunotoxicity, disease resistance. the t cell response was lower in the high exposure groups. antibody mediated, and innate immune respons ... | 2013 | 23639742 |
| unusual coliranuloma in very young japanese quail chick. | a commercial japanese quail farmer reported death from 8 to 30% in three different flocks of 3 days old age, 3 weeks old age and 4 weeks old age of total flock size 18800. investigation was under taken to find out the cause of death. post mortem examination followed by bacteriological, virological and histopathological examinations revealed spontaneous colibacillosis. e. coli serotypes o24, o6 and o128 were isolated in pure culture. isolates were strong congo red binder and sensitive to enterofl ... | 2013 | 24517033 |
| effects of recombinant lycopene dietary supplement on the egg quality and blood characteristics of laying quails. | this study was conducted to determine the effect of dietary supplement of bacterial lycopene (bl) produced by escherichia coli on the egg quality and blood characteristics of laying quails. the antioxidant activity measurement showed that bl exhibited 100% 1,1-diphenyl-2-picrylhydrazyl (dpph) scavenging capacity at a concentration of 4.65 μg/ml, which was more effective than butylated hydroxytoluene (bht) and commercial lycopene (cl). moreover, seven dietary groups of laying quails consisting of ... | 2015 | 26100321 |
| effect of a single polymorphism in the japanese quail nk-lysin gene on antimicrobial activity. | nk-lysins are cationic peptides that play important roles in host protection, and are an important constituent of innate immunity. we identified nine single-nucleotide polymorphisms (snps) in the nk-lysin open reading frame (orf) from 32 japanese quails in six strains: a, b, nd, k, p, and y. the g to a substitution at nucleotide position 272 in the orf resulted in a gly (g) to asp (d) amino acid substitution (cj31g and cj31d alleles). the cj31d allele was detected in p (frequency 0.76) and y (fr ... | 2016 | 26472627 |
| nigella sativa (black cumin seed) as a biological detoxifier in diet contaminated with aflatoxin b1. | aflatoxin b1 (afb1 ) is a common feed contaminant that adversely affects bird performance and product quality. a total of 600 7-day-old quail chicks were randomly allotted to eight experimental groups in a completely randomized design with five replicate pens and 15 quails per pen. experimental treatments including two levels of afb1 (0 and 2.5 mg/kg) and 4 levels of nigella sativa (ns) (0, 0.5, 1.0 and 1.5% of diet) were offered from 7 to 35 days of age to quail chicks. although feeding of afb1 ... | 2016 | 27553624 |
| Validation of a disease model in Japanese quail (Coturnix coturnix japonica) with the use of Escherichia coli serogroup O2 isolated from a turkey. | This study established a disease model and protocol for bacterial challenge with Escherichia coli serogroup O2 strain EC317 in Japanese quail. Five groups of 10 birds each were injected subcutaneously in the breast with 200 µL of a brain-heart infusion (BHI) culture containing 1 × 10(8), 1 × 10(7), 1 × 10(6), 1 × 10(5), or 1 × 10(4) colony-forming units/mL of the test organism, which had been isolated from a turkey with cellulitis and septicemia. Birds in a 6th group were controls that received ... | 2011 | 22210992 |
| immunotoxicity and disease resistance in japanese quail (corturnix coturnix japonica) exposed to malathion. | the purpose of this study was to evaluate the impact of malathion on the immune system of wild birds, using japanese quail (coturnix coturnix japonica) as a model. quail were exposed to malathion in drinking water at environmentally realistic concentrations (0 ppm, 1 ppm, and 10 ppm). in the fifth week, several arms of the immune response were tested using the t-cell based phytohemagglutinin (pha) skin test, the b-cell mediated antibody response, and the chemiluminescence assay measuring innate ... | 2011 | 21448623 |
| effectiveness of phytogenic feed additive as alternative to bacitracin methylene disalicylate on hematological parameters, intestinal histomorphology and microbial population and production performance of japanese quails. | this study was conducted to evaluate the effects of phytogenic additive and antibiotic growth promoter in laying japanese quails. one hundred and sixty five quails were divided into three groups of 5 replicates and 11 quails (8 females and 3 males) in each replicate. treatment 1 was fed control diet, treatment 2 was fed control diet supplemented with 0.05% bacitracin methylene disalicylate as antibiotic growth promoter and treatment 3 was fed control diet supplemented with 0.1% phytogenic feed a ... | 2016 | 27189636 |