| nucleotide sequence variability in the nontranscribed spacer of the rrna locus in the oyster parasite perkinsus marinus. | we examined the sequence variability of the nontranscribed spacer (nts) and internal-transcribed spacer (its1 and its2) domains of the rrna locus of perkinsus marinus from maryland, florida, and louisiana. the sequence of p. marinus dna including the 5s rrna, nts, small subunit (ssu) rrna, itsi, and its2 regions confirmed their contiguity in the rrna locus and revealed differences at 28 positions with the ssu rrna sequences published earlier. the 307-bp polymerase chain reaction (pcr)-amplified ... | 1999 | 10461944 |
| polymorphism at the its and nts loci of perkinsus marinus isolated from cultivated oyster crassostrea corteziensis in nayarit, mexico and phylogentic relationship to p. marinus along the atlantic coast. | prevalence of the protozoan perkinsus spp. in the gills of the pleasure oyster crassostrea corteziensis from two estuaries in nayarit, mexico, was measured. the protozoan was identified by pcr amplification of the internal transcribed spacer (its) region of the rdna of perkinsus spp. the pathogen was found in 92% of oysters from boca de camichín and 77% of oysters from pozo chino. its sequences characterized from c. corteziensis showed 96-100% similarity to perkinsus marinus. the most frequent i ... | 2015 | 23607743 |